Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,931,904 C→A 7.6% G733V (GGC→GTC)  PP_2566 ← leucine‑rich repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,931,9040CA 7.6% 160.0 / 2.6 52G733V (GGC→GTC) PP_2566leucine‑rich repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (27/21);  new base A (2/2);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

CTCTCGAACAGCGCCTCGCGATGCTGTTCCAGGTGTTGCGCGAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGCCACCCCGGCAGGCGCAGCAACT  >  NC_002947/2931757‑2932051
                                                                                                                                                   |                                                                                                                                                   
ctctCGAACAGCGCCTCGCGATGCTGTTCCAGGTGTTGCGCGAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGcc                                                                                                                                                    <  1:75236/149‑1 (MQ=255)
 tctcGAACAGCGCCTCGCGATGCTGTTCCAGGTGTTGCGCGAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCa                                                                                                                                                   >  7:71381/1‑149 (MQ=255)
 tctcGAACAGCGCCTCGCGATGCTGTTCCAGGTGTTGCGCGAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATACAGCACGGATTGGCTCAACTGGCCa                                                                                                                                                   >  8:31173/1‑149 (MQ=255)
   tcGAACAGCGCCTCGCGATGCTGTTCCAGGTGTTGCGCGAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCa                                                                                                                                                   <  1:209554/147‑1 (MQ=255)
     gAACAGCGCCTCGCGATGCTGTTCCAGGTGTTGCGCGAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCAtt                                                                                                                                                 <  4:241905/147‑1 (MQ=255)
     gAACAGCGCCTCGCGATGCTGTTCCAGGTGTTGCGCGAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCAtt                                                                                                                                                 >  3:241905/1‑147 (MQ=255)
                   gATGCTGTTCCAGGTGTTGCGCGAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCAtt                                                                                                                                                 >  4:105022/1‑133 (MQ=255)
                   gATGCTGTTCCAGGTGTTGCGCGAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCAtt                                                                                                                                                 <  3:105022/133‑1 (MQ=255)
                            ccAGGTGTTGCGCGAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTc                                                                                                                         >  6:3361/1‑148 (MQ=255)
                                   ttGCGCGAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTtct                                                                                                                  <  8:71381/148‑1 (MQ=255)
                                      cgcgAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTtctgtct                                                                                                              >  6:249158/1‑149 (MQ=255)
                                      cgcgAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTtctgtct                                                                                                              >  4:327633/1‑149 (MQ=255)
                                       gcgAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCAAGGTCGGTACGGGAAATTTCGATTtctgtctg                                                                                                             >  6:46271/1‑149 (MQ=255)
                                         gAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGc                                                                                                           <  3:327633/149‑1 (MQ=255)
                                         gAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGc                                                                                                           <  5:249158/149‑1 (MQ=255)
                                                  gttCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCgtgt                                                                                                  >  6:214233/1‑149 (MQ=255)
                                                   ttCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCgtgt                                                                                                  >  3:148603/1‑148 (MQ=255)
                                                      aGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAg                                                                                               <  4:148603/148‑1 (MQ=255)
                                                        tGCGGACGCTCGGTGCTCGGTTGGTGTCTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCGAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGTGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAgggg                                                                                            <  5:46271/149‑1 (MQ=255)
                                                                          ggtTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAggg                                                                                             >  3:117779/1‑130 (MQ=255)
                                                                          ggtTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAggg                                                                                             <  4:117779/130‑1 (MQ=255)
                                                                           gtTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCAc                                                                               <  2:10957/143‑1 (MQ=255)
                                                                           gtTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCAc                                                                               >  1:10957/1‑143 (MQ=255)
                                                                                     cACCGAGCAACAGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGt                                                               <  7:142853/149‑1 (MQ=255)
                                                                                      aCCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGtt                                                              >  2:177737/1‑149 (MQ=255)
                                                                                                  cTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTg                                                    <  1:177737/147‑1 (MQ=255)
                                                                                                   tCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGt                                                  >  7:112478/1‑148 (MQ=255)
                                                                                                     aaTGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCACGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCAAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTaaa                                               >  7:111804/1‑149 (MQ=255)
                                                                                                      aTGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTg                                                    >  8:138305/1‑143 (MQ=255)
                                                                                                      aTGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTg                                                    <  7:138305/143‑1 (MQ=255)
                                                                                                           ttcttcATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCtt                                           >  5:92571/1‑147 (MQ=255)
                                                                                                              ttcATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCCGGCCCTTg                                                    <  1:84522/135‑1 (MQ=255)
                                                                                                              ttcATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCACAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCCGGCCCTTg                                                    >  2:84522/1‑135 (MQ=255)
                                                                                                                   ccatcTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAACCCTTCAGCACGTTc                                 >  4:222997/1‑149 (MQ=255)
                                                                                                                    catcTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTc                                 <  8:112478/148‑1 (MQ=255)
                                                                                                                    catcTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTc                                 <  5:214233/148‑1 (MQ=255)
                                                                                                                     atcTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTgg                                                          >  3:105501/1‑122 (MQ=255)
                                                                                                                     atcTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTgg                                                          <  4:105501/122‑1 (MQ=255)
                                                                                                                            ccAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTATCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGc                        >  4:300982/1‑149 (MQ=255)
                                                                                                                             cAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGc                        <  5:3361/148‑1 (MQ=255)
                                                                                                                              aGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGCCa                      >  6:165452/1‑149 (MQ=255)
                                                                                                                              aGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGCCa                      >  6:79915/1‑149 (MQ=255)
                                                                                                                                 aCGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGCCAccc                   >  8:40562/1‑149 (MQ=255)
                                                                                                                                           tCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGCCACCCCgg                <  5:295510/142‑1 (MQ=255)
                                                                                                                                           tCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGCCACCCCgg                >  6:295510/1‑142 (MQ=255)
                                                                                                                                            gaccTGGACAATGGCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTc                                 <  4:144111/121‑1 (MQ=255)
                                                                                                                                            gaccTGGACAATGGCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTc                                 >  3:144111/4‑124 (MQ=255)
                                                                                                                                            gaccTGGACAATGGCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGCCa                      <  2:54106/132‑1 (MQ=255)
                                                                                                                                            gaccTGGACAATGGCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGCCa                      >  1:54106/4‑135 (MQ=255)
                                                                                                                                              aCTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGCCACCCCGGCAGGCgcagc      <  6:92571/149‑1 (MQ=255)
                                                                                                                                                  gCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCgg                          <  2:18041/125‑1 (MQ=255)
                                                                                                                                                  gCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCgg                          >  1:18041/1‑125 (MQ=255)
                                                                                                                                                  gCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGCCACCCCGGCAGGCGCAGCAACt  >  5:177701/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTCTCGAACAGCGCCTCGCGATGCTGTTCCAGGTGTTGCGCGAGTTTGTTGTTCAGTGCGGACGCTCGGTGCTCGGTTGGTGTGTCACCGAGCAACTGCTCAATGGCTTCTTCATCCATCTGATCCAGCACGGTTTGGCTCAACTGGCCATTGTCCAGGTCGGTACGGGTAATTTCGATTTCTGTCTGGCCCAGCGTGTGAGGGGGGCCGTAGCGCACCGATTCGCCCCAGGGTTCTGGCCCTTGGTAAACCTTCAGCACGTTCCCCTCGGGCCACCCCGGCAGGCGCAGCAACT  >  NC_002947/2931757‑2932051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑