Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 294,971 T→C 7.4% V125V (GTT→GTC)  ssuB → aliphatic sulfonates ABC transporter ATP‑binding protein SsuB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947294,9710TC 7.4% 151.6 / 2.5 54V125V (GTT→GTC) ssuBaliphatic sulfonates ABC transporter ATP‑binding protein SsuB
Reads supporting (aligned to +/- strand):  ref base T (26/24);  new base C (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

GTGCCGCGCCGCTGGAAGAGGCCCGTGAAGAAACCCGCCTGATGTTCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCCTGCTGCTGCTGGACGAGCCGCTGGGGGCGCTGGATGCGTTGACCCGTAT  >  NC_002947/294823‑295117
                                                                                                                                                    |                                                                                                                                                  
gTGCCGCGCCGCTGGAAGAGGCCCGTGAAGAAACCCGCCTGATGTTCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGtt                                                                                                                                                    <  7:66893/149‑1 (MQ=255)
       gccgcTGGAAGAGGCCCGTGAAGAAACCCGCCTGATGTTCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTtggcctgg                                                                                                                                             <  4:39958/149‑1 (MQ=255)
              gAAGAGGCCCGTGAAGAAACCCGCCTGATGTTCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACcg                                                                                                                                      >  8:239966/1‑149 (MQ=255)
              gAAGAGGCCCGTGAAGAAACCCGCCTGATGTTCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGACGCGGTCGGCCAGGCCAACcg                                                                                                                                      >  2:218426/1‑149 (MQ=255)
                 gagGCCCGTGAAGAAACCCGCCTGATGTTCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCgcgc                                                                                                                                   >  3:144324/1‑149 (MQ=255)
                   ggCCCGTGAAGAAACCCGCCTGATGTTCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGACGCGGTCGGCCAGGCCAACcgcatcc                                                                                                                                 <  1:218426/149‑5 (MQ=255)
                    gCCCGTGAAGAAACACGCCTGATGTTCCGGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACACCGTTGGCCTTGGGCTGTCTGGAGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCaa                                                                                                                                >  7:289119/1‑149 (MQ=255)
                             gaaACCCGCCTGATGTTCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGcc                                                                                                                       >  4:187707/1‑149 (MQ=255)
                                        gATGTTCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGctct                                                                                                            >  7:265217/1‑149 (MQ=255)
                                         aTGTTCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCtctc                                                                                                           <  3:187707/149‑1 (MQ=255)
                                            ttCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCgg                                                                                                         <  8:309898/148‑1 (MQ=255)
                                            ttCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCgg                                                                                                         <  7:239966/148‑1 (MQ=255)
                                             tCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCgg                                                                                                                     >  5:335002/1‑135 (MQ=255)
                                             tCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCgg                                                                                                                     <  6:335002/135‑1 (MQ=255)
                                              ccAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTTCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACTAATGGCCGGCAGCGCTCTCGGGa                                                                                                       <  8:73600/148‑1 (MQ=255)
                                                aGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGc                                                                                                    >  3:128093/1‑149 (MQ=255)
                                                     gcgcgGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAg                                                                                                 >  1:69810/1‑147 (MQ=255)
                                                          gctgctGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAagcagc                                                                                          <  6:129868/149‑1 (MQ=255)
                                                                             gTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGc                                                                       <  2:69810/149‑1 (MQ=255)
                                                                              tGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGcc                                                                      >  4:127307/1‑149 (MQ=255)
                                                                                aTCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCt                                                                     <  3:127307/148‑1 (MQ=255)
                                                                                     cAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGa                                                                  >  1:98767/1‑146 (MQ=255)
                                                                                     cAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGa                                                                  <  2:98767/146‑1 (MQ=255)
                                                                                         gTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCtt                                                                    <  5:74375/140‑1 (MQ=255)
                                                                                         gTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCtt                                                                    >  6:74375/1‑140 (MQ=255)
                                                                                                  gggCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGAtt                                                                >  6:110284/1‑135 (MQ=255)
                                                                                                  gggCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGAtt                                                                <  5:110284/135‑1 (MQ=255)
                                                                                                    gCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCa                                                           <  7:98017/138‑1 (MQ=255)
                                                                                                    gCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCa                                                           >  8:98017/1‑138 (MQ=255)
                                                                                                        tCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCctgctg                                              <  8:265217/147‑1 (MQ=255)
                                                                                                          tGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGc                                                                             <  1:27155/114‑1 (MQ=255)
                                                                                                          tGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGc                                                                             >  2:27155/1‑114 (MQ=255)
                                                                                                          tGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCctgctgctgc                                          >  3:249172/1‑149 (MQ=255)
                                                                                                          gggCGCCTGGCGCCCGCGGGCGCTGGAGGGCGTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCctgctgctgc                                          <  4:185309/148‑1 (MQ=255)
                                                                                                            gCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCctgctgctgctg                                        >  4:49505/1‑149 (MQ=255)
                                                                                                              gACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCCTGCTGCTGCTGGa                                      >  6:145875/1‑149 (MQ=255)
                                                                                                               aCTGGCGCCCGCGTGAGCTGGAGGCCTTGTATGCGGTTGGCCTGGCCGACCGCGGCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCTCCTGCTGCTGCTGGAc                                     <  8:289119/149‑1 (MQ=255)
                                                                                                                      cccGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCctgctgctgc                                          >  1:335205/1‑137 (MQ=255)
                                                                                                                      cccGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCctgctgctgc                                          <  2:335205/137‑1 (MQ=255)
                                                                                                                      cccGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCCTGCTGCTGCTGGACGAGCCGc                              >  2:264368/1‑149 (MQ=255)
                                                                                                                                   gAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGAt                                                                 >  7:119701/1‑101 (MQ=255)
                                                                                                                                   gAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGAt                                                                 <  8:119701/101‑1 (MQ=255)
                                                                                                                                    aGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCCTGCTGCTGCTGGACGAGCCGCTGGGGGCGCTGGAt                <  3:49505/149‑1 (MQ=255)
                                                                                                                                     ggCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCCTGCTGCTGCTGGACGAGCCGCTGGGGGCGCTGGATg               <  1:264368/149‑1 (MQ=255)
                                                                                                                                        cTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCctgctgctg                                           <  2:256135/118‑1 (MQ=255)
                                                                                                                                        cTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCctgctgctg                                           <  2:251462/118‑1 (MQ=255)
                                                                                                                                        cTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCctgctgctg                                           >  1:256135/1‑118 (MQ=255)
                                                                                                                                        cTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCctgctgctg                                           >  1:251462/1‑118 (MQ=255)
                                                                                                                                          tGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCCTGCTGCTGCTGGACGAGCCGCTGGGGGCGCTGGATGCGtt           >  8:314833/1‑148 (MQ=255)
                                                                                                                                            ggcgCGGTCGGCCAGGCCAACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCt                                                                     >  6:267065/4‑88 (MQ=38)
                                                                                                                                            ggcgCGGTCGGCCAGGCCAACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCt                                                                     <  5:267065/85‑1 (MQ=38)
                                                                                                                                                 ggTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCCTGCTGCTGCTGGACGAGCCGCTg                            <  3:216473/124‑1 (MQ=255)
                                                                                                                                                 ggTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCCTGCTGCTGCTGGACGAGCCGCTg                            >  4:216473/1‑124 (MQ=255)
                                                                                                                                                 ggTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCCTGCTGCTGCTGGACGAGCCGCTGGGGGCGCTGGATGc              <  6:255316/138‑1 (MQ=255)
                                                                                                                                                 ggTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCCTGCTGCTGCTGGACGAGCCGCTGGGGGCGCTGGATGc              >  5:255316/1‑138 (MQ=255)
                                                                                                                                                  gTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCCTGCTGCTGCTGGACGAGCCGCTGGGGGCGCTGGATGCGTTGACCCGTAt  >  8:259373/1‑149 (MQ=255)
                                                                                                                                                   ttGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGccc                                                                           >  4:299136/1‑75 (MQ=255)
                                                                                                                                                   ttGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGccc                                                                           <  3:299136/75‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTGCCGCGCCGCTGGAAGAGGCCCGTGAAGAAACCCGCCTGATGTTCCAGGACGCGCGGCTGCTGCCGTGGAAGAAGGTGATCGACAACGTTGGCCTTGGGCTGTCTGGCGACTGGCGCCCGCGTGCGCTGGAGGCCTTGGATGCGGTTGGCCTGGCCGACCGCGCCAACGAATGGCCGGCAGCGCTCTCGGGAGGCCAGAAGCAGCGCGTGGCTTTGGCCCGAGCCTTGATTCACCAGCCCCGCCTGCTGCTGCTGGACGAGCCGCTGGGGGCGCTGGATGCGTTGACCCGTAT  >  NC_002947/294823‑295117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑