Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,946,587 G→C 8.9% P312R (CCG→CGG) ‡ yijP ← phosphoethanolamine transferase CptA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,946,5870GC 8.9% 129.8 / 3.8 45P312R (CCG→CGG) ‡yijPphosphoethanolamine transferase CptA
Reads supporting (aligned to +/- strand):  ref base G (18/22);  new base C (2/2);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

TCTGGTTGCGCTGGTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTGG  >  NC_002947/2946441‑2946731
                                                                                                                                                  |                                                                                                                                                
tCTGGTTGCGCGGGTTGTTCAGGTACAGCGGCGCGTCTGTTTGCTGCGAGAAGGTGGATAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACgg                                                                                                                                                 <  7:127197/148‑1 (MQ=255)
        cgcTGGTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGTGATGGGGGTGATGATGGTGTTGAGCTTGGTGAAGGTCTGCTTGTTGGTGTTCCAGAAGGTCTTGTAGCCTGCATGTTTCAGCAGGTTTATCAGCGACGGGTctgtga                                                                                                                                         <  8:119534/148‑6 (MQ=255)
        cgcTGGTTGTTCAGGGACACCGGCGCGTCTGGTTGCTGCGAGAAGGGGCTGAGCATGGTGTTGCGCTTGGTCAATGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGa                                                                                                                                         <  6:269019/148‑1 (MQ=255)
              ttgttCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACg                                                                                                                                  >  1:184538/1‑149 (MQ=255)
                    aGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACgg                                                                                                                                                 <  2:316996/128‑1 (MQ=255)
                    aGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACgg                                                                                                                                                 >  1:316996/1‑128 (MQ=255)
                                  gTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGc                                                                                                              <  2:184538/149‑1 (MQ=255)
                                   tCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGcc                                                                                                             <  6:332654/149‑1 (MQ=255)
                                       tttGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAAc                                                                                                         >  3:62841/1‑149 (MQ=255)
                                           ctgcGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGa                                                                                                     <  3:313166/149‑1 (MQ=255)
                                            tgcGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAg                                                                                                    >  7:23766/1‑149 (MQ=255)
                                              cGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGc                                                                                                                     >  6:362963/1‑130 (MQ=255)
                                                  aaGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCgg                                                                                                                            <  2:99886/119‑1 (MQ=255)
                                                  aaGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGACTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCgg                                                                                                                            >  1:99886/1‑119 (MQ=255)
                                                        gtgAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCAt                                                                                        >  7:189348/1‑149 (MQ=255)
                                                              aTGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTc                                                                                  >  1:338849/1‑149 (MQ=255)
                                                               tGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGa                                                                                                     >  2:143005/1‑129 (MQ=255)
                                                               tGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGa                                                                                                     <  1:143005/129‑1 (MQ=255)
                                                                 gtgtTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATAACTTCGAt                                                                               >  7:52882/1‑149 (MQ=255)
                                                                    ttGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGgt                                                                            <  4:62841/149‑1 (MQ=255)
                                                                    ttGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGgt                                                                            >  4:294559/1‑149 (MQ=255)
                                                                    ttGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGgt                                                                            <  8:324300/149‑1 (MQ=255)
                                                                    ttGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGgt                                                                            >  3:180665/1‑149 (MQ=255)
                                                                        gcTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTAc                                                                        <  2:338849/149‑1 (MQ=255)
                                                                            ggTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACgg                                                                      <  8:189348/147‑1 (MQ=255)
                                                                               cAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGAt                                                                               <  5:228159/135‑1 (MQ=255)
                                                                               cAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGAt                                                                               >  6:228159/1‑135 (MQ=255)
                                                                                aaGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCgcgg                                                                >  5:167587/1‑149 (MQ=255)
                                                                                aaGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCgcgg                                                                <  6:358491/149‑1 (MQ=255)
                                                                                 aGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACTGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCgcggc                                                               <  1:235552/149‑1 (MQ=255)
                                                                                  ggTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCgcggc                                                               >  6:189941/1‑148 (MQ=255)
                                                                                    tCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGa                                                             >  3:159259/1‑148 (MQ=255)
                                                                                                     cAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATc                                                                                                >  6:37317/1‑96 (MQ=255)
                                                                                                     cAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATc                                                                                                <  5:37317/96‑1 (MQ=255)
                                                                                                         aGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTcgc                                                  <  5:140487/138‑1 (MQ=255)
                                                                                                         aGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTcgc                                                  >  6:140487/1‑138 (MQ=255)
                                                                                                         aGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTc                                       <  1:135748/149‑1 (MQ=255)
                                                                                                              ttGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATAc                                                          <  3:178964/125‑1 (MQ=255)
                                                                                                              ttGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATAc                                                          >  4:178964/1‑125 (MQ=255)
                                                                                                                gTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGctc                                 <  3:294559/148‑1 (MQ=255)
                                                                                                                                          tCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAgg      <  5:189941/149‑1 (MQ=255)
                                                                                                                                          tCAGCGACCCGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGa                                                             >  2:161302/1‑94 (MQ=255)
                                                                                                                                          tCAGCGACCCGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGa                                                             <  1:161302/94‑1 (MQ=255)
                                                                                                                                          tCAGCGACCCGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAgc               <  2:288487/140‑1 (MQ=255)
                                                                                                                                          tCAGCGACCCGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAgc               >  1:288487/1‑140 (MQ=255)
                                                                                                                                             gCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTg   <  4:159259/149‑1 (MQ=255)
                                                                                                                                             gCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTg   <  4:180665/149‑1 (MQ=255)
                                                                                                                                              cGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTgg  <  8:23766/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCTGGTTGCGCTGGTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTGG  >  NC_002947/2946441‑2946731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑