| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 93.1% | 81.7 / 0.5 | 29 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (17/10); total (19/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01 | |||||||||||
CTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATT > NC_002947/1932500‑1932780 | cTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGGCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAg < 3:68291/148‑1 (MQ=255) ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAgg > 4:71876/1‑148 (MQ=255) aaGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCCACCCCCCTCCGACAAAGGCAATTGGTCCCCCGGAGGAGCGGGCGT‑GCCCGCGACCGGCTg > 1:156515/1‑148 (MQ=255) aaGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTATGAGCGGGCTT‑GCCCGCGAAGGGCt > 8:46040/1‑147 (MQ=255) gagGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAACCCGCTCCGACAAAGGGTATGGGTCCCCGGTAGGAGCGGGCGT‑GCCCGCGAAGGGCTGCa > 5:135310/1‑148 (MQ=38) aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAACCCCCACCGACAAAGGATATGCGGGCCCGGTAGGAGCGGGCGT‑GCCCGCGCAGGGCTGCAACGCccccccc > 2:76326/1‑146 (MQ=255) ttCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCaaa > 4:22938/1‑148 (MQ=255) gACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTATGCGGCCCTTCGCGGGCAAGCCCGCAGCGACAAACGATATGCCTCCCCTGTAGGAGCGGGCAT‑GCCCCCGCACGGCAGCAAGGACGCCCCAAAATg > 7:162994/1‑148 (MQ=255) ccgCGGCTTTGCAGGTCTCACTAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCAACAAAGTATATGGGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 4:89118/146‑1 (MQ=37) cAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGCATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 8:6014/148‑1 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCGGTAGGAGCGGGCTTGG‑CCGCGCAGGGCTGGCAGGCAGCCCCAAATTGTCGACTACGCGGGTCAGCGAt > 2:54786/1‑148 (MQ=38) aGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCTTTTGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 2:37095/148‑1 (MQ=38) aGCAGAACACGCGGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAACGTATAGGCGTCCCCTGTAGGAGCGGGCTG‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 3:19899/148‑1 (MQ=37) aGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCGGTAGGCGCGGGCTT‑GCCCGCGCAGGGCTGCAAGGCAGCCCCAAATTGTCGCCTACGCGGCTCAGCGATCCGGCCGGGTg > 7:121726/1‑148 (MQ=37) gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCGCCGACAAAGGATATGCGGCCCCGGTAGGAGCGGGCTT‑GCCCGCGCAGGGCAGGAAGGCAGCCCCCAATTTTAGCCTACGCCGATAAGCGATCCCGCAGGGGGa > 8:157923/1‑148 (MQ=21) tAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCCAACCCCCAACAACAAACGCTATGCGGGCCCAGTAGGAGCGGGCTTGG‑CCCCCAACGGCTGCAAGGCCGCCCCCAATTGTCCACAACGCGGAAAACCGAACCGGCaccgggaa > 6:509/1‑141 (MQ=255) aaccgcgGGGGCCGCTTGGCGGCCCTTCGCGGGCACGCCCGCGCCTACAACGGATAGGCGGCCCCTGTCGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg < 5:78559/145‑1 (MQ=37) aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCCGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGt > 8:23840/1‑148 (MQ=255) aCGCGGGGGCGGCTTTGCGGCCTTTCGGGGGAAAGCCGGATCATACAAAGTATATGGGTCCCTTTTAGTTGCGGGGTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt < 2:46234/148‑1 (MQ=255) gCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc < 3:71876/148‑1 (MQ=255) cGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGc > 5:7365/1‑148 (MQ=255) gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCt > 3:67671/1‑148 (MQ=255) tACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata > 2:199780/1‑148 (MQ=255) tatGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatca > 3:51435/1‑148 (MQ=255) gTCCCCTGTTGGTGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt < 1:9218/148‑1 (MQ=255) ccccTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc > 5:150017/1‑148 (MQ=255) cTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa > 4:30793/1‑148 (MQ=255) gttgggggCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATTCACATCATCGTTGCCGATGtt < 6:145880/142‑1 (MQ=255) gCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAtt > 8:52421/1‑148 (MQ=255) | CTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATT > NC_002947/1932500‑1932780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |