Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 307,866 +C intergenic (+881/‑988) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947307,8651.C95.7% 151.2 / ‑0.7 46intergenic (+880/‑989)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (20/24);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCA  >  NC_002947/307720‑308003
                                                                                                                                                   |                                                                                                                                          
gCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑cc                                                                                                                                          <  6:190718/148‑2 (MQ=255)
      ttCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTCTCGGCTGACGAAA‑GCCGTTAcc                                                                                                                                     >  2:47752/1‑148 (MQ=255)
       tctcGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTAcc                                                                                                                                     <  5:24155/147‑1 (MQ=255)
                aGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTga                                                                                                                           <  2:34768/148‑1 (MQ=255)
                 gAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTgac                                                                                                                          >  3:8699/1‑148 (MQ=255)
                 gAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTga                                                                                                                           >  1:87236/1‑147 (MQ=255)
                  aaCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTgacg                                                                                                                         >  7:123796/1‑148 (MQ=255)
                  aaCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTgacg                                                                                                                         >  3:110510/1‑148 (MQ=255)
                                ggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTgg                                                                                                           <  2:151983/148‑1 (MQ=255)
                                 gctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTggg                                                                                                          <  4:183429/148‑1 (MQ=255)
                                  cttccgATGTACTGCCTATGCACTGCGCGGCCAACATAGGCGTAGAAGGCGATGTGAGCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTCCGCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTgggg                                                                                                         <  6:98715/145‑1 (MQ=255)
                                             cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTg                                                                                              >  4:172034/1‑148 (MQ=255)
                                              tGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGt                                                                                             <  1:185764/148‑1 (MQ=255)
                                                 cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTCTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCtt                                                                                          <  1:47752/148‑1 (MQ=255)
                                                     tcACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                       <  7:653/146‑1 (MQ=255)
                                                     gCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                       <  8:20344/147‑1 (MQ=255)
                                                       aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACa                                                                                     <  4:8699/147‑1 (MQ=255)
                                                       aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACAt                                                                                    >  2:192666/1‑148 (MQ=255)
                                                                 cAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCACCATGGAAggcggc                                                                          >  5:163384/1‑148 (MQ=255)
                                                                 cAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcggc                                                                          >  2:160485/1‑148 (MQ=255)
                                                                  aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgct                                                                         <  1:141174/148‑1 (MQ=255)
                                                                         gCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACg                                                                   >  5:36604/1‑147 (MQ=255)
                                                                           gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc                                                                  >  5:111303/1‑146 (MQ=255)
                                                                                 ggCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAATC‑CGTTAACTGATCGGTGACGACGAGCACGGATGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAACTGCa                                                          >  3:168994/1‑148 (MQ=255)
                                                                                   cGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATc                                                        <  6:111303/148‑1 (MQ=255)
                                                                                      tgtgACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                     <  4:91491/148‑1 (MQ=255)
                                                                                            ctggTTCTTCGTCCTGTCCGGCACGGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCGGAGAGGTGCCGACGCGCACGGTGGGGGCGAAGGCGTTGTATTCAACATGGCAGGCGGCTGCTCCGCCACGTGAATCTACCGGTc                                                >  8:178897/4‑147 (MQ=255)
                                                                                            cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                               <  1:192666/148‑1 (MQ=255)
                                                                                               gTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCga                                             >  2:189574/1‑147 (MQ=255)
                                                                                                 gcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaa                                          <  6:47066/147‑1 (MQ=255)
                                                                                                   ttcGGCCTGTCCTGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAATGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaag                                         <  7:134584/147‑1 (MQ=255)
                                                                                                   ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAATATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaaga                                        >  8:19333/1‑148 (MQ=255)
                                                                                                    tcGGCGTGTCCGGCACTGGCAAGACTACGCTTTCGGGTGACGAAAGG‑CGTTACCTGATCGGTGAGGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACTCCAAGTGCATCGACCTGTCCGAgaagaa                                       <  5:127853/148‑1 (MQ=255)
                                                                                                      ggCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                       >  7:117808/1‑146 (MQ=255)
                                                                                                      ggCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                       <  8:123796/146‑1 (MQ=255)
                                                                                                           gTCCGGCACTGTCAAGACTACCCTTTCGGCGGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGAGAAGGCGTTGTCTTCAACATTGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                                <  2:102246/148‑1 (MQ=255)
                                                                                                            tCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCgg                               >  7:11945/1‑148 (MQ=255)
                                                                                                             ccGGCACTTGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                                <  7:97840/146‑1 (MQ=255)
                                                                                                             ccGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                                <  1:164202/146‑1 (MQ=255)
                                                                                                               ggCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTc                             <  4:130310/147‑1 (MQ=255)
                                                                                                                        aaGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAg                   >  6:20625/1‑148 (MQ=255)
                                                                                                                        aaGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAg                   >  6:137577/1‑148 (MQ=255)
                                                                                                                             tACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc              <  4:34111/148‑1 (MQ=255)
                                                                                                                                     cGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACg       <  4:27122/147‑1 (MQ=255)
                                                                                                                                        cTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCaa            >  1:164394/1‑139 (MQ=255)
                                                                                                                                         tGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTTGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAATAACGAGCAGGTCATCTGGAAAGCCATCAAGCACGGCGCa  >  2:110705/1‑148 (MQ=255)
                                                                                                                                         tGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg           <  2:164394/139‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
GCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCA  >  NC_002947/307720‑308003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑