| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,126,647 | +C | intergenic (‑101/+48) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 89.7% | 86.4 / 4.2 | 29 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base C (11/15); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.96e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGTA‑AA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126511‑1126787 | gccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCCCGAAAGCGGAGGGATATCCCCCCACGCATTAGCGGGGTA‑AC‑CCCCTccgaaac > 7:85766/1‑143 (MQ=255) gACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCCCGCATGCGGGGGGTTAGCCCCCATAGCAATAGCGGGGTA‑AC‑CCCCGCCGACACACCGCCCCCCCCcccacccac > 3:45900/1‑147 (MQ=255) gCTCTGGGACATGGGGACCTCCTGCCAGGTTGTTGGGTGCACATTGCCCCTGGTCGGCGCTGTTTTACCCGCGAATGCGGCGTTATACCCACCATCCCATGCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTg < 4:192163/148‑1 (MQ=25) gTGACATGTGGACCTCCTCCCAGGTTGTTGGGGGGACATTGACCCCTGTAGGCGCGGGTTTCCCCGCGAATGCGGAGGTATCGCCACCATCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCg < 1:66606/146‑1 (MQ=37) gtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTAACCCCAAAGCGGAGGGATATCCACCAAAGCAATAGC‑CGGTA‑AACCCGCACCGACAAACAGCCCCCCCCCCAACCCACGTGGCGGGGAGGGcg > 4:70457/1‑147 (MQ=255) aGGTTGGTGGGGGGACATTGAGCCCTGTAGGCGCGGGGTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATGCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa < 7:115567/148‑1 (MQ=38) ggTTGTTGGGGGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGAt < 4:135662/148‑1 (MQ=255) gttgttGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATa < 5:99782/148‑1 (MQ=255) tggttgGGGGCACATTGCGCCCTGTAGGCGCGGGTTTACCCGCTAATGCGGAGGTATAGCCACCATCGCATGCGC‑GGTAA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAg < 5:951/146‑1 (MQ=255) ggACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGCGGTATCGCCACCATCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGGCCTGATAGCTCtcgatc < 3:132948/148‑1 (MQ=37) ttGAGCCCTGTAGGCGCGGGTTTAACCCCCAAAGCGGAGGGATAGCCACCAAAGCAATAGC‑GGGTA‑AACCCCCACCAAAACAAAGCCCCCCCCCCAAACCACGGGGCGGGGAGGGCTAAAAGGCCTCCGGAAATCACTAGAACGAccg > 8:38537/1‑146 (MQ=255) aGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGc < 3:158677/147‑1 (MQ=255) ttggggggggTTTACCCGCGATGGCGGTGGAATCCCCCCCATCGATTGCGG‑GGTTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGcac < 2:81501/141‑1 (MQ=255) gcgcGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCacac < 5:69283/147‑1 (MQ=255) gggTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGt > 8:152296/1‑148 (MQ=255) gTTTCCCCGCGAAGGCGGAGGTATAGCCACCAGCGCATGCGG‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTg < 1:29868/148‑1 (MQ=255) tttACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGc > 8:114363/1‑148 (MQ=255) tttACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGc > 4:99665/1‑148 (MQ=255) ttACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCg < 7:38537/148‑1 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCg > 3:59453/1‑148 (MQ=255) gaataCCCACCATCGCTTGCGC‑GGTTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTc < 5:111834/146‑1 (MQ=255) taGCCACCATCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAc > 6:52264/1‑148 (MQ=255) aGCCACCATCGCATTCGC‑GGGTACAC‑CCGCTCCTACACAGAGCCCGCCACCCACGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACCCCAGGTTGCGGTCGCCGAACACGTTGTCGAcc > 7:102398/1‑148 (MQ=255) ccaccaTCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAccc < 3:191977/147‑1 (MQ=255) caccaTCGCATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg < 4:172202/148‑1 (MQ=255) cATTCGC‑GGGTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcgg > 4:127821/1‑148 (MQ=255) gggTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCa > 8:50176/1‑147 (MQ=255) gTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTa > 7:153574/1‑148 (MQ=255) gTA‑AACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTa > 2:17662/1‑148 (MQ=255) | GCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGGTA‑AA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126511‑1126787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |