Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,635,568	A→C	L63R (CTG→CGG)	uvrY ←	BarA/UvrY two‑componenent system response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,635,568	0	A	C	100.0%	107.1 / NA	35	L63R (CTG→CGG)	uvrY	BarA/UvrY two‑componenent system response regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/14);  total (21/14)

GTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGG  >  NC_002947/4635429‑4635712                                                                                                                                            |                                                                                                                                                 gTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCTGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGccgccg                                                                                                                                          >  6:193874/1‑148 (MQ=255) gTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGgccgcc                                                                                                                                           <  3:116863/147‑1 (MQ=255) gTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGccgccg                                                                                                                                          >  3:88463/1‑148 (MQ=255)  tCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGgccg                                                                                                                                             >  4:73922/1‑144 (MQ=255)  tCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGa                                                                                                                                         <  4:184797/148‑1 (MQ=255)   cAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGgccgc                                                                                                                                            <  3:73922/144‑1 (MQ=255)    aaGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGCACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATg                                                                                                                                       >  3:129014/1‑148 (MQ=255)        ccTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCgg                                                                                                                                   <  4:99568/148‑1 (MQ=255)                 ttGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTca                                                                                                                          <  3:173175/148‑1 (MQ=255)                    gTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACCCCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCCTTTTcccct                                                                                                                       >  1:54052/1‑144 (MQ=255)                              cGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGTTGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGcaa                                                                                                             <  6:193113/148‑2 (MQ=255)                                        ggCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGt                                                                                                   <  6:79365/148‑1 (MQ=255)                                               aacaaGCGTGTGGGGACCGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACCTCCATCAGCACAACGTCCGGTTTCAGCt                                                                                            >  7:134256/1‑148 (MQ=255)                                                   aGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCg                                                                                        <  3:157254/148‑1 (MQ=255)                                                    gCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCgg                                                                                       >  8:9494/1‑148 (MQ=255)                                                               aCGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAg                                                                            >  8:5314/1‑148 (MQ=255)                                                                 gggTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTg                                                                          >  6:32621/1‑148 (MQ=255)                                                                          tCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGc                                                                 >  8:174322/1‑148 (MQ=255)                                                                          tCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGc                                                                 >  4:185706/1‑148 (MQ=255)                                                                                  cGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAAtct                                                         <  8:53020/148‑1 (MQ=255)                                                                                      aCCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTccc                                                      >  2:68406/1‑147 (MQ=255)                                                                                           cACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAAtc                                                          >  2:185487/1‑138 (MQ=255)                                                                                           cACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTcaccc                                                >  5:35627/1‑148 (MQ=255)                                                                                            aCTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAAtct                                                         <  1:185487/138‑1 (MQ=255)                                                                                                        tCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGc                                   >  5:170554/1‑148 (MQ=255)                                                                                                        tCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTACCTCACCCACCACCTGCAGGc                                   >  5:174325/1‑148 (MQ=255)                                                                                                          aGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCg                                 >  2:153500/1‑148 (MQ=255)                                                                                                               ggCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCCCCACCTGCAGGCCGTCGAt                            >  7:79500/1‑148 (MQ=255)                                                                                                               ggCTGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGAt                            <  7:5314/148‑1 (MQ=255)                                                                                                                  tGCGCAGCAGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTc                         >  4:32581/1‑148 (MQ=255)                                                                                                                     gcagcaGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCg                        <  5:193874/146‑1 (MQ=255)                                                                                                                     gcagcaGTTTGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCg                        <  1:68406/146‑1 (MQ=255)                                                                                                                             ttGCGGGTGGCCTCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATcc              <  4:129014/148‑1 (MQ=255)                                                                                                                                         tCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGACTCTCCCTCACCCACCACCTGCCGGCCGTCGATGTCGGCCAACATCCGAGTAATACCgg  >  7:94536/1‑148 (MQ=255)                                                                                                                                         tCCCGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCCCCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCgg  >  8:5627/1‑148 (MQ=255)                                                                                                                                            |                                                                                                                                                 GTCAAGGCCTGCACCCTTGGTCAGGTAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACATCCATCAGCACAACGTCCGGTTTCAGCTCCCGGGCCAGTTTCAGTGCCGACTCGCCGGAATCTCCCTCACCCACCACCTGCAGGCCGTCGATGTCGGCCAACATCCGTGTAATACCGG  >  NC_002947/4635429‑4635712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑