Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	47.1 / NA	15	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/9);  total (6/9)

CACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCG‑CGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTC  >  NC_002947/499069‑499309                                                                                                                                       |                                                                                                            cACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgctc                                                                                                <  1:175992/148‑3 (MQ=255)  aCCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgctcc                                                                                               <  6:33198/148‑4 (MQ=255)    ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACg                                                                                             >  8:8310/1‑148 (MQ=255)     cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTACCGCGCAccg                                                                                            >  5:136647/1‑146 (MQ=255)       caAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggc                                                                                          <  6:27130/148‑1 (MQ=255)                ccTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCctgc                                                                                 <  6:19294/148‑1 (MQ=255)                                 aaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGa                                                                 <  1:160643/147‑1 (MQ=255)                                   gTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAAGCCCGCGAACGGGCCTGGCCTTCTGCTCGCTATGGCGGAGcc                                                              >  3:27288/1‑148 (MQ=255)                                        cTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAAGCCCGCGAACGGGCCTGGCCTGCTGAACTCTATGGCTGCTCCGGcca                                                         >  8:96695/1‑147 (MQ=255)                                           gCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAATCCCCCCAAACGGCCAGGCCAGCTGATATATATTGCTGAGCCCGCctcat                                                      >  2:1890/1‑146 (MQ=255)                                                cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCG‑Cgg                                                <  1:184773/148‑1 (MQ=255)                                                 ggAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCG‑Cggg                                               <  6:121841/148‑1 (MQ=255)                                                                            cacgCTGATCTTATTGCGACATAGGGGGGCCCGAACCTTGCCGACGCCTTTGGGGGCGCGGGCTTGCCCGCTACGGGGCCTGGCCTGCTGATCTCTATGGCGGAGCCGGCCTCTGCG‑CGGGCATGCCCGCTCCCACAGGTTTCGTGGt                    <  4:69263/145‑1 (MQ=255)                                                                            caAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCCAACGGGCCTGGCCGGCGGGTCTCTATTGCGGGGCCGGCCGCATAGCCGGGCAGGCCC‑CACCCACACGGTTggtggt                    >  6:193414/1‑148 (MQ=255)                                                                                              aCATACGGGGGCCCTAAAGTTGCCTATGCCTTTGGGGGAGGGGGCATGCCGGCTAAGGGGCCTGGCGTGGTGATCTCTTTGGCTGAGCGGGCCTCTTCG‑CGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTc  <  4:27288/148‑1 (MQ=25)                                                                                                                                       |                                                                                                            CACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCG‑CGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTC  >  NC_002947/499069‑499309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑