Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,948,143	A→C	F111V (TTC→GTC)	rho ←	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,948,143	0	A	C	100.0%	128.0 / NA	39	F111V (TTC→GTC)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/20);  total (19/20)

TCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAAC  >  NC_002947/5948010‑5948286                                                                                                                                      |                                                                                                                                                tCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCcct                                                                                                                                    <  7:111629/147‑1 (MQ=255) tCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCcct                                                                                                                                    <  4:98043/147‑1 (MQ=255)     tGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCccttcctt                                                                                                                               >  5:133653/1‑148 (MQ=255)     tGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCccttcctt                                                                                                                               >  4:36828/1‑148 (MQ=255)             ttACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGc                                                                                                                       <  6:11169/148‑1 (MQ=255)             ttACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGc                                                                                                                       <  3:90331/148‑1 (MQ=255)               aCCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCgg                                                                                                                     >  5:42094/1‑148 (MQ=255)                             tCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGAt                                                                                                       >  4:112205/1‑148 (MQ=255)                               aGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCCGGCGGTCGAAGGTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATg                                                                                                      <  7:38184/147‑1 (MQ=255)                                                          aGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAAcggc                                                                          >  2:164284/1‑148 (MQ=255)                                                           ggTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTGAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAAcggc                                                                          <  8:35286/147‑1 (MQ=255)                                                           ggTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAAcggc                                                                          <  3:36828/147‑1 (MQ=255)                                                           ggTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACAATGGTGTCGCCGGTACGCAGGTTGAAAcggcg                                                                         >  3:25936/1‑148 (MQ=255)                                                           ggTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCCACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGAGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAAcggc                                                                          >  3:52475/1‑147 (MQ=255)                                                            gTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCACTTCCTTCGGTGGGCGGTTCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAAcggcgg                                                                        <  8:24392/148‑1 (MQ=255)                                                                   aaCAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATctggc                                                                 >  8:5305/1‑148 (MQ=255)                                                                          tCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTggg                                                             >  3:36012/1‑145 (MQ=255)                                                                          tCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTggg                                                             >  7:3537/1‑145 (MQ=255)                                                                               ttcttcGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACa                                                      <  3:176138/147‑1 (MQ=255)                                                                               ttcttcGCGTTTTCCGGGCGGGCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCgaga                                                        <  2:18452/145‑1 (MQ=255)                                                                                tcttcGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACAt                                                     <  4:63958/147‑1 (MQ=255)                                                                                tcttcGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATa                                                    <  5:101010/148‑1 (MQ=255)                                                                                tcttcGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATa                                                    <  6:42684/148‑1 (MQ=255)                                                                                        ttttCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGt                                            >  3:166536/1‑148 (MQ=255)                                                                                          ttCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCt                                          >  7:97908/1‑148 (MQ=255)                                                                                           tCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTg                                         >  4:185867/1‑148 (MQ=255)                                                                                              gggCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGGCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTggg                                       <  1:158963/147‑1 (MQ=255)                                                                                                  ggTAGAAGTTGATGGTATCGACCTTCAGAAGGGCGACGTAACGCTCACCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGAAGGTTGAAACGGCGGATCTGGCTGCGCGAGACATAGATATCGTCTGggccgg                                   >  4:156258/1‑147 (MQ=255)                                                                                                   gTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGggccgg                                   >  8:132224/1‑146 (MQ=255)                                                                                                     cGAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCTGTACGCAGGTTGAAACGGCGGATATGGCTGGGCGATACATAGATATCGTCTGGGCCGGCCAg                               <  3:156258/148‑1 (MQ=255)                                                                                                      gAAGTTGATGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAgg                              >  6:82698/1‑148 (MQ=255)                                                                                                        aGTTGATGGTAGCGACCTTCAGCACGGCGACGTACCGCTCCCCTGCATTCGGTGGGCGGATCTTGCCGACGATTGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTa                            <  7:87825/148‑1 (MQ=255)                                                                                                             aTGGTATCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATCTCGTCTGGGCCGGCCAGGTAGGAc                        >  7:120822/1‑147 (MQ=255)                                                                                                                  aTCGCCCTTCAGCAGGGCGACGTAACGCTCCCCTTGCTTCGGAGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCa                  >  2:87404/1‑148 (MQ=255)                                                                                                                  aTCGACCTTCAGCAGGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGGCATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCa                  <  4:87956/148‑1 (MQ=255)                                                                                                                         ttCAGCAGGGCGACGTAACGCTCCACTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGACGAAc           >  4:167450/1‑148 (MQ=255)                                                                                                                             gcagGGCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAg       <  6:42094/148‑1 (MQ=255)                                                                                                                                 ggCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGgaaa   <  8:3537/148‑1 (MQ=255)                                                                                                                                  gCGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGgaaac  <  7:5305/148‑1 (MQ=255)                                                                                                                                      |                                                                                                                                                TCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCAGGAAAC  >  NC_002947/5948010‑5948286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑