| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 81.8% | 41.8 / 6.8 | 25 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base C (7/11); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 96.0% | 53.1 / ‑5.9 | 25 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (9/15); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑‑T‑CGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAT > NC_002947/1499361‑1499640 ||| gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGACCGACGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CAcg‑c < 3:2072/148‑1 (MQ=25) ggAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CAcg‑ccc < 7:401/147‑2 (MQ=37) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CAcg‑cccgctc > 7:151903/1‑143 (MQ=25) ccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAGGCGCCGCGGGGCATGTCACCCGCGTTGCCGGTGT‑T‑‑TGCGGG‑CCcg‑cccgcgccc > 1:87849/1‑141 (MQ=25) ccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CCcg‑cccgcgccc > 3:140760/1‑141 (MQ=25) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCCGCTTTGCCGGTGT‑TC‑G‑CCGG‑CACGCCCGCTCCCAAAGACTGATTTCCATTAAGcc > 8:153621/1‑147 (MQ=21) aCGAAGCCTGGGCACCACAGCAGGACTGAGGCGGCCAAACGGGGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCGGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 1:155464/147‑1 (MQ=21) gCCTTGCCACCCCCGCCGCCCTGAGGCTGCAAAAGGGTGGGAGCGGGCGGCCCCGGTAAACTGGCGCCGCGGGGATGGGCCCCGGCTTTGCGGGTGT‑G‑‑G‑GGGGCCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 8:65936/148‑1 (MQ=255) tGAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑Gttttt < 5:89337/148‑1 (MQ=21) ggCTGCCAAACGGTGGGTTGGGGCGTGCACGCGAAAACGGCGCGGCGGTTCATGGAACGGGGTTTGCCGGGGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAATCGTGCCTATCGCTACAGTGACGTCCC‑Gtttttt < 4:163586/146‑1 (MQ=255) ggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑Gtttttt < 6:84121/146‑1 (MQ=21) gCTGCCTAAGGGTGGGCGCGGGCGTGCCCGCGAAACCGGCGCCGCGGGGAATGGCACCGGCTTGGCCGGTGTGC‑‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTttgtt < 7:6161/148‑1 (MQ=18) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTttgtt < 8:151903/148‑1 (MQ=21) aaaCGGTGGGAGCGGGCGTGCCCCCCAAACACGCGCCCCGGTGAATGGCACCCGCTTTGGCGGTGT‑TA‑G‑CGGGCCCCC‑CCGCGCCCCCACACTGATTGCCAATAAGCCGGCCGATCGCTAAAGTGACCGCCCGGTTTTTTTTTGcgc > 2:113712/1‑145 (MQ=255) aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATTGCACCCGCTTTGCCGGTGT‑TC‑G‑CGGGCCCCC‑CCGCTCCCACAGACTGATTGCCATAAACCGGGCCTATCGCTACACTGACGGCCC‑GGTTTTTGTTGCCTcc > 6:94508/1‑146 (MQ=18) gAGCGGGCGTGCCCGCGAAAAACGCGCCCCGGTGCATGGCACCCGCGTTTCCGGTGT‑TA‑G‑CGGG‑CACGCCCGCGACCACAGACAGATTTCCATTAAGCGGGCCTATAGCGACAGTGACGGCCC‑GGTTTTTGTTTCCTCGCTAACGaa > 8:136342/1‑147 (MQ=16) ggggggggCCGGCTAAACTGGCG‑CGGGGTGGCTTGCCACGGGTTTGGCGGTTT‑TGCG‑CGGG‑AACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCAt < 2:87849/141‑1 (MQ=255) cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCAt < 7:153621/148‑1 (MQ=25) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa > 7:64919/1‑99 (MQ=17) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTAc < 8:64919/99‑1 (MQ=17) gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa > 1:67322/1‑148 (MQ=37) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt > 7:152587/1‑148 (MQ=37) cgGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg < 1:113712/148‑1 (MQ=37) gCATGGCACGGGCTTGGCCGGTGT‑TC‑G‑CGGG‑AACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATTACTCCCTTAGAACGCTATCAAGCAGATCt < 8:137010/148‑1 (MQ=255) gggCTTTGCGGGTGTGC‑‑G‑CGGG‑AACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcc < 5:100927/147‑1 (MQ=255) gttcgcGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt < 1:19005/143‑1 (MQ=255) gttcgcGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt > 7:165914/6‑148 (MQ=255) ||| GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑‑T‑CGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAT > NC_002947/1499361‑1499640 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |