| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 92.9% | 66.8 / ‑6.0 | 28 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base A (10/16); total (10/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 96.4% | 64.1 / ‑6.4 | 28 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (10/17); total (10/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑‑T‑CGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCT > NC_002947/1499347‑1499618 || cGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgt < 5:45049/148‑1 (MQ=255)cGCAGGGCGACAACGCTGAAGCCCGTGATGCGGCCCTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgt < 5:146706/148‑1 (MQ=38) aGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑t < 5:152829/148‑1 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgcg < 6:98158/147‑5 (MQ=255) gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGACCGACGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CAcg‑c < 3:2072/148‑1 (MQ=25) ggAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CAcg‑ccc < 7:401/147‑2 (MQ=37) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CAcg‑cccgctc > 7:151903/1‑143 (MQ=25) ccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAGGCGCCGCGGGGCATGTCACCCGCGTTGCCGGTGT‑T‑‑TGCGGG‑CCcg‑cccgcgccc > 1:87849/1‑141 (MQ=25) ccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CCcg‑cccgcgccc > 3:140760/1‑141 (MQ=25) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCCGCTTTGCCGGTGT‑TC‑G‑CCGG‑CACGCCCGCTCCCAAAGACTGATTTCCATTAAGcc > 8:153621/1‑147 (MQ=21) aCGAAGCCTGGGCACCACAGCAGGACTGAGGCGGCCAAACGGGGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCGGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 1:155464/147‑1 (MQ=21) gCCTTGCCACCCCCGCCGCCCTGAGGCTGCAAAAGGGTGGGAGCGGGCGGCCCCGGTAAACTGGCGCCGCGGGGATGGGCCCCGGCTTTGCGGGTGT‑G‑‑G‑GGGGCCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 8:65936/148‑1 (MQ=255) tGAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑Gttttt < 5:89337/148‑1 (MQ=21) ggCTGCCAAACGGTGGGTTGGGGCGTGCACGCGAAAACGGCGCGGCGGTTCATGGAACGGGGTTTGCCGGGGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAATCGTGCCTATCGCTACAGTGACGTCCC‑Gtttttt < 4:163586/146‑1 (MQ=255) ggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑Gtttttt < 6:84121/146‑1 (MQ=21) gCTGCCTAAGGGTGGGCGCGGGCGTGCCCGCGAAACCGGCGCCGCGGGGAATGGCACCGGCTTGGCCGGTGTGC‑‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTttgtt < 7:6161/148‑1 (MQ=18) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTttgtt < 8:151903/148‑1 (MQ=21) aaaCGGTGGGAGCGGGCGTGCCCCCCAAACACGCGCCCCGGTGAATGGCACCCGCTTTGGCGGTGT‑TA‑G‑CGGGCCCCC‑CCGCGCCCCCACACTGATTGCCAATAAGCCGGCCGATCGCTAAAGTGACCGCCCGGTTTTTTTTTGcgc > 2:113712/1‑145 (MQ=255) aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATTGCACCCGCTTTGCCGGTGT‑TC‑G‑CGGGCCCCC‑CCGCTCCCACAGACTGATTGCCATAAACCGGGCCTATCGCTACACTGACGGCCC‑GGTTTTTGTTGCCTcc > 6:94508/1‑146 (MQ=18) gAGCGGGCGTGCCCGCGAAAAACGCGCCCCGGTGCATGGCACCCGCGTTTCCGGTGT‑TA‑G‑CGGG‑CACGCCCGCGACCACAGACAGATTTCCATTAAGCGGGCCTATAGCGACAGTGACGGCCC‑GGTTTTTGTTTCCTCGCTAACGaa > 8:136342/1‑147 (MQ=16) ggggggggCCGGCTAAACTGGCG‑CGGGGTGGCTTGCCACGGGTTTGGCGGTTT‑TGCG‑CGGG‑AACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCAt < 2:87849/141‑1 (MQ=255) cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCAt < 7:153621/148‑1 (MQ=25) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa > 7:64919/1‑99 (MQ=17) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTAc < 8:64919/99‑1 (MQ=17) gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa > 1:67322/1‑148 (MQ=37) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt > 7:152587/1‑148 (MQ=37) cgGTGCATGGCACCGGCTTTGCCGGTGT‑TC‑G‑CGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg < 1:113712/148‑1 (MQ=37) gCATGGCACGGGCTTGGCCGGTGT‑TC‑G‑CGGG‑AACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATTACTCCCTTAGAACGCTATCAAGCAGATCt < 8:137010/148‑1 (MQ=255) || CGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑‑T‑CGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCC‑GTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCT > NC_002947/1499347‑1499618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |