| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,508 | +C | intergenic (+85/‑79) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,506 | 1 | . | C | 97.9% | 142.1 / ‑2.9 | 48 | intergenic (+83/‑81) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (24/23); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCG‑GTGT‑T‑T‑CGGGCA‑CG‑CCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG > NC_002947/1499360‑1499649 | cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑cg‑c < 6:227143/149‑1 (MQ=37) cTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCCCGGTGCATGGCACCCGCATTGCCG‑GTGT‑TCG‑CGGGCA‑cg‑ccc > 6:232215/1‑148 (MQ=37) aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑cg‑cccgctc < 6:143446/149‑6 (MQ=25) tCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑At > 5:89525/1‑149 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCgg < 7:235043/149‑1 (MQ=21) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGGCCGCGAAACAGGCGCCGCCGGGCATGGGACCGGGGTTGCCG‑GTGT‑T‑TGCGGGGACCG‑CCGCGCGCACACACGGAGTGGC‑AGTTAGgggg > 8:184573/1‑149 (MQ=255) aaCGAAGCCTTGGCACCCCAGCAGGACTGAGGCTGCCAAACGGTGGGTGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGGTTTGCCGCGTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCgg < 1:328421/147‑1 (MQ=21) aCGAAGCCTTGTCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCt < 1:154173/149‑1 (MQ=21) aCGAAGCCTGGCCACCACCGCCGCACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAACCGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCt < 3:214173/149‑1 (MQ=18) ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCCCAAAACGCGCCCCGGGGCATGGCACCCGCGTTTCCC‑GTGT‑TAG‑CGGGGA‑CGCCCGCACCCCCAAACAGAATTGC‑AATAAACCGGCCGATCGc > 4:156209/1‑148 (MQ=255) cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGTCGCCGCTGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGc < 5:317120/140‑1 (MQ=21) ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCCGCTTTGCCG‑GTGT‑TAG‑CGGGCA‑CGCCCGCGCCCCCAGCCTGATTGCC‑ATTAATCGGGCCGATCGCTACAGTGAc > 2:294059/1‑149 (MQ=21) ctgcagGACGGCGGCTGCCAAACGGTGGGAGCGGGCGGGCCCGCGAAACCGGCGCCGCGGGGCATGGCACCGGCTTTGCCG‑GTGTGG‑G‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGc < 4:317636/147‑1 (MQ=21) agcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAACGCGCCGCGGTGCAAGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGGC‑CGCCCGCGCCCACAGCCTGAATGGC‑ATTAAGCGGGGCGATCGCTAAAGTGAAGGcc > 2:129224/1‑149 (MQ=12) gcagGACTGCGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGGGGTGCATGGCACGGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGccc < 2:148559/149‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCCCAGACTGATTGCC‑ATTAAGCGGGCCTATCGCGACAGTGACGGCCCGTGTTttgg > 8:296967/1‑148 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 8:210883/147‑1 (MQ=21) ggcggcCAAACGGTGGTCGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCCG‑GTGTGC‑G‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 3:174555/149‑1 (MQ=25) ctatcGGTGGGAGCGGGGGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCg < 1:408250/145‑1 (MQ=21) cGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa < 2:28336/149‑1 (MQ=21) gtaGCGGGCGGGCCCGCTAAACTGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑GGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt < 8:354884/147‑1 (MQ=21) aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TGG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 5:232215/149‑1 (MQ=25) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 5:341876/149‑1 (MQ=25) cGGGGGTGCCCGCTAAACAGGGGCCGCGGTGCATGGCACCGGCTTTTCCG‑GTGT‑TCG‑CGGTAA‑CGCCCGCTCCCAAAGACTGATTGCCTCTTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 1:129224/149‑1 (MQ=21) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 3:156209/149‑1 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 6:89525/148‑1 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 2:146626/1‑147 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 3:157894/1‑147 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑CTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTCACGGCCCGTGTTTAGTTGCCTCGATAACGAATAATGACTCCCt > 4:317578/1‑146 (MQ=21) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 3:364154/1‑148 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 3:250945/1‑148 (MQ=37) aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 5:428203/144‑1 (MQ=37) aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa > 6:428203/1‑144 (MQ=37) aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc > 5:197704/1‑149 (MQ=37) aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc > 4:394060/1‑148 (MQ=37) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa > 2:270427/1‑149 (MQ=37) aGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa > 4:15621/1‑148 (MQ=37) gcgcCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 4:285019/1‑148 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg < 4:250945/149‑1 (MQ=37) cgcgGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 7:48621/1‑147 (MQ=37) cgcgGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCa < 4:157894/149‑1 (MQ=37) gTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATc < 1:294059/149‑1 (MQ=38) aTGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGCGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGa > 6:18362/1‑148 (MQ=37) tggcaccGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaaa > 3:446222/7‑149 (MQ=38) ggcaccGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAAc > 2:30063/6‑149 (MQ=38) gCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGa > 3:414673/1‑149 (MQ=39) gcGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgc > 2:230210/2‑149 (MQ=255) gcGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcg < 4:446222/147‑1 (MQ=255) cGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgcg > 6:170751/1‑149 (MQ=255) gCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGCCTTCTTCCATGACgcggcg > 3:28006/1‑149 (MQ=255) gCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcggcg < 1:230210/149‑1 (MQ=255) | CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCG‑GTGT‑T‑T‑CGGGCA‑CG‑CCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG > NC_002947/1499360‑1499649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |