Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,932,640 +C intergenic (‑107/+43) rluA ← / ← minE bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,932,6381.C98.3% 201.4 / ‑4.8 61intergenic (‑105/+45)rluA/minEbifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base C (31/28);  minor base A (1/0);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

GCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTG‑CCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATT  >  NC_002947/1932496‑1932780
                                                                                                                                                 |                                                                                                                                               
gcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGGCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTG‑cccgcg                                                                                                                                           <  3:383588/149‑5 (MQ=255)
 cgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTG‑cccgcga                                                                                                                                          <  7:388811/149‑6 (MQ=255)
     ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCCTTGCAGCCCTTCGCGGGCAAGCCCGCACCTACACGGGAAGCATAACCATT‑GTAGGAGCGGGC‑ATGACCGCGAAggg                                                                                                                                       >  1:95309/1‑149 (MQ=25)
        gAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑Tg                                                                                                                                   >  8:250809/1‑149 (MQ=255)
           gagTATCTGGACATTCGACAGCGGCTTTGCAGGTATTACTAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAATTATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCaa                                                                                                                                <  6:383315/149‑1 (MQ=37)
              gATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGc                                                                                                                             <  5:236890/149‑1 (MQ=255)
                    gACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGccc                                                                                                                        >  5:331/1‑148 (MQ=255)
                         tCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCCAACCCCCTCCTAAAAAGGGTATTCGTGCCCG‑GTGTGAGCGGGCGTGG‑CCGCGCAGGGCAGGCAAGGC‑GCCCCaaaat                                                                                                                  >  4:13007/1‑147 (MQ=255)
                         tCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGGTATGCGTCCCCG‑GTTGGAGCGGGG‑GTGCCCGCGAAGGGC‑GGGAAGGCAGCCCCACAtt                                                                                                                  >  4:415645/1‑149 (MQ=38)
                              cgCGGCATTGCAGGTCTCACGAAAATAGCAGTAACCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGGCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGa                                                                                                             <  3:368106/148‑1 (MQ=255)
                                cGGCATTGCCGGTCTCACGAAAACAGCAGTAAACGCGGGGCCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCGCCTACAAAGGATAGCCGCCCCCT‑GTAGGAGCGGGC‑TGGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACt                                                                                                           <  3:95977/149‑1 (MQ=37)
                                  gCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTAc                                                                                                         <  3:386235/149‑1 (MQ=255)
                                        cAGGCCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGAt                                                                                                   >  8:248871/1‑149 (MQ=255)
                                           gTCTCACTAAAATAGAAGTAAACGCTGGGGCCGCTTTGCGGCCGTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCT‑GTAGGAGGGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa                                                                                                 <  4:171300/148‑1 (MQ=37)
                                           gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg                                                                                                >  5:394322/1‑149 (MQ=255)
                                              tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGa                                                                                             >  7:337468/1‑149 (MQ=255)
                                               cACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAt                                                                                            >  8:46860/1‑149 (MQ=255)
                                                 cGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCAACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATcc                                                                                          <  5:216869/149‑1 (MQ=255)
                                                               accgcgGGGGCCGCTTGGGGGCCCTTCGCGGGCACCCCCGCTCAAACAAAGTATAGGCGCCCCCT‑GTTGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt                                                                            <  3:415645/147‑1 (MQ=37)
                                                               aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCG‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGGCCCCAATTGTCGACTACGCGGATCAGCGATGCGGCAGGGTGATGtt                                                                            >  1:254958/1‑149 (MQ=37)
                                                                  gcgggggCGGCTGTGGGCCCGTGCGCGGGAACCCCCCCTCATACAACGTATATGCGGCCCCT‑GTCGGAGGGGGC‑TGGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg                                                                         <  8:174407/146‑1 (MQ=255)
                                                                      gggCCGGTTGGCGGCCCTGCGCGGGCACCCCCGCTCCTACAACGTATATGCGCCCCCT‑GTCGGCGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTcc                                                                     <  3:201458/149‑1 (MQ=37)
                                                                       ggCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGGTATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTc                                                                      >  2:347189/1‑147 (MQ=255)
                                                                       ggCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa                                                                    <  7:46860/149‑1 (MQ=255)
                                                                        gCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCACCAACAAAGGATATGCGTCCCCA‑GTAAGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAAATGTAGACAACGCCGATCAGCCATCCCGCAGGGTGATGTTGAGCTGCa                                                                    >  2:344437/1‑148 (MQ=25)
                                                                         ccGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAg                                                                   >  5:419850/1‑148 (MQ=255)
                                                                           gCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAAt                                                                >  3:135354/1‑149 (MQ=255)
                                                                               tGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGa                                                             <  8:337468/148‑1 (MQ=255)
                                                                               ggCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑TTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGa                                                             <  1:344437/147‑1 (MQ=255)
                                                                                gCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGag                                                            <  4:135354/148‑1 (MQ=255)
                                                                                  ggCCGTTCGCGGGCAAGCCCGCTCATACAAAGTATATGCGTCCCGT‑TTAGTAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcag                                                         <  6:331/149‑1 (MQ=38)
                                                                                       ttCGCGGGCAAGCCCGCTCCTAAAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGGAAGGCAGCCCCAAATTGTCGACTACGCGGAGAAGGGATGCGGGAGGGTGATGGTCAGCTGCAGAATAGGGCAGCTGcg                                                    >  4:304774/1‑148 (MQ=37)
                                                                                              gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa                                                                    >  7:78983/1‑126 (MQ=255)
                                                                                              gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa                                                                    <  8:78983/126‑1 (MQ=255)
                                                                                              gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGttt                                             <  1:347189/149‑1 (MQ=255)
                                                                                               cAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt                                            >  3:86074/1‑149 (MQ=255)
                                                                                                   cccGCTCCTACAAAGGATATGCGTCCCCTGGTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGTTCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc                                                               >  1:272394/1‑127 (MQ=38)
                                                                                                   cccGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGTTCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc                                                               <  2:272394/126‑1 (MQ=255)
                                                                                                    ccGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGc                                       >  1:276170/1‑149 (MQ=255)
                                                                                                          cTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat                                  <  7:248871/148‑1 (MQ=255)
                                                                                                          cTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat                                  <  6:394322/148‑1 (MQ=255)
                                                                                                            aCAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg                               >  4:109437/1‑149 (MQ=255)
                                                                                                              aaaGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc                                                                           <  4:102216/103‑1 (MQ=255)
                                                                                                              aaaGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc                                                                           >  3:102216/1‑103 (MQ=255)
                                                                                                              aaaGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca                             >  2:118968/1‑149 (MQ=255)
                                                                                                                    tatGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc                                           <  6:367994/129‑1 (MQ=255)
                                                                                                                    tatGCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc                                           >  5:367994/1‑129 (MQ=255)
                                                                                                                       gCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatcat                       <  7:273521/146‑1 (MQ=255)
                                                                                                                       gCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatcat                       >  8:273521/1‑146 (MQ=255)
                                                                                                                       gCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt                    >  1:352441/1‑149 (MQ=255)
                                                                                                                       gCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt                    >  3:245559/1‑149 (MQ=255)
                                                                                                                       gCGTCCCCT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt                    >  8:12591/1‑149 (MQ=255)
                                                                                                                          tccttt‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg                  <  8:77993/143‑1 (MQ=255)
                                                                                                                            cccT‑GTCGGCGCGGGC‑GTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCg               <  3:13007/149‑1 (MQ=39)
                                                                                                                            cccT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTACCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc                >  2:154809/1‑148 (MQ=255)
                                                                                                                              cT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcac                            <  5:327430/134‑1 (MQ=255)
                                                                                                                              cT‑GTAGGAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcac                            >  6:327430/1‑134 (MQ=255)
                                                                                                                                     gAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca                             <  3:420733/127‑1 (MQ=255)
                                                                                                                                     gAGCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca                             >  4:420733/1‑127 (MQ=255)
                                                                                                                                       gCGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcaca      >  7:356442/1‑148 (MQ=255)
                                                                                                                                        cGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca        >  6:77025/1‑145 (MQ=255)
                                                                                                                                        cGGGC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca        <  5:77025/145‑1 (MQ=255)
                                                                                                                                          ggC‑TTGCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAtt  >  8:220638/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCT‑GTAGGAGCGGGC‑TTG‑CCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATT  >  NC_002947/1932496‑1932780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑