Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	5,681,415	+CGGG	intergenic (‑133/+71)	PP_4986 ← / ← PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5681415	5 (0.060)	42 (0.560) +CGGG	35/272	2.0	89.6%	intergenic (‑133/+71)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein
	?	NC_002947	5681416 =	5 (0.060)					intergenic (‑134/+70)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5681270‑5681415 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGC  >  NC_002947/5681416‑5681557                                                                                                                                                    ||||                                                                                                                                                CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCG                                                                                                                                                   <  2:369805/148‑1  ACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGG                                                                                                                                                  <  7:75515/148‑1    CGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGC                                                                                                                                               >  8:420335/1‑149    CGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTGCATTGCCACTGCATGAACCGCCGGGGC                                                                                                                                               >  4:271699/1‑149             TGCAAACACAGACCTCCTGCGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCA                                                                                                                                      <  8:68727/149‑1              GCAATCACTGACCTCCTGAGGTTGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTGCGTTCTTTGTGGGAGCGGCCTTGTGGCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCA                                                                                                                                      <  8:392115/148‑1                gAACCCAGCCCACCTCAGGAGGTCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCAATGCCCCTGCATGTACCGCCGGGGCAGCTGCGCAG‑CC                                                                                                                                  >  5:133753/2‑149                AAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CC                                                                                                                                  <  8:36358/149‑1                AAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CC                                                                                                                                  >  3:356607/1‑149                AAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTCCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CC                                                                                                                                  <  3:271699/149‑1                AAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCGTTCATTGGCACTGCATGAACCGCCGGGGCGTCTGCGCAG‑CC                                                                                                                                  >  4:406756/1‑149                       GACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGC                                                                                                                           <  7:110227/149‑1                           TCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACA                                                                                                                       >  1:78174/1‑149                             CTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACAC                                                                                                                      <  2:286355/148‑1                             CTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACAC                                                                                                                      <  1:110124/148‑1                              TGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCCTTTCGCGA                                                                                                                         <  8:338838/145‑1                              TGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCCTTTCGCGA                                                                                                                         >  7:338838/1‑145                                AGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGG                                                                                                                  <  4:356607/149‑1                                AGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAA                                                                                                                    <  1:267764/147‑1                                     GGCTGGGTTCCTGTTGTGATGCTGATGGTCTTCGTTCTTTGTGGGAGCGGCCTTGTGTGGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCAATAGCGCCACAAGGCCGC                                                                                                              >  1:257856/1‑149                                          GGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG                                                                                                                                     >  1:172397/1‑121                                          GGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG                                                                                                                                     <  2:172397/121‑1                                                             ATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCT                                                                                                             >  4:54900/1‑125                                                             ATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCT                                                                                                             <  3:54900/125‑1                                                                      GTTATTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCA                                                                              <  7:420335/148‑1                                                                                      GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGT                                                               >  5:82843/1‑147                                                                                        CTTGTGTCGCGAAGGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCCG‑CCCTATCGCGCCACAAGGCCGCTCCCCCTCCAGACCACCAGGCGCTGGGCCTCACCTCCTAACCAAGGTCAGG                                                           >  7:73063/1‑149                                                                                             GTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTG                                                        >  5:408048/1‑147                                                                                                  GAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATTAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCC                                                 <  6:441704/149‑1                                                                                                               AGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCTAAAAAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCAC                                      <  7:412880/147‑1                                                                                                                 CGGCCCCTGGCTTTATTTGCCACTGCATGACCCGCCGGGGCTGCTGCGCAG‑CCCTATCGCTACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGC                                  <  3:93099/149‑1                                                                                                                     CCCTGGCTTAAATTGCCACTGAATCACCCGCGGGGGCTGCTGCGCAG‑CCCTATCGCCAAAAAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTT                              <  1:332450/149‑1                                                                                                                     CCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTT                              <  6:427314/149‑1                                                                                                                        AGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCAGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGCTTGCAGAACCGCGTCCCGCACCAGCCTTTCC                            >  5:166910/1‑148                                                                                                                          GCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCT                                                                                >  4:68137/1‑94                                                                                                                          GCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCT                                                                                <  3:68137/94‑1                                                                                                                               CATTCCCACTGAATGACCCCCCGGGGCGGCTGCGCAG‑CCCTAGCGCCAAAAAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGA                     <  3:406756/148‑1                                                                                                                                ATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACC                   <  6:408048/149‑1                                                                                                                                ATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAC                    >  5:111359/1‑148                                                                                                                                 ttcCCACTGCAGGAACCGCCGGGGGGGCTGCGAAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCA                  <  8:249674/146‑1                                                                                                                                          CATGAACCGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGT          <  6:82843/148‑1                                                                                                                                                 CGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGC  <  7:275882/149‑1                                                                                                                                                 CGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCA                                    <  2:204442/115‑1                                                                                                                                                 CGCCGGGGCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCA                                    >  1:204442/1‑115                                                                                                                                                    ||||                                                                                                                                                CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGG‑CTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5681270‑5681415 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGCTGCGCAG‑CCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGC  >  NC_002947/5681416‑5681557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.