Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,753	G→C	intergenic (+115/+25)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,753	0	G	C	100.0%	265.9 / NA	77	intergenic (+115/+25)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (39/38);  total (39/38)

CCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGC‑G‑CGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674612‑5674890                                                                                                                                                 |                                                                                                                                          ccAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCg                                                                                                                                     <  4:447845/149‑1 (MQ=255)  cAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCgg                                                                                                                                    <  2:236914/149‑1 (MQ=255)    gcgcTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGtt                                                                                                                                  <  4:362338/149‑1 (MQ=255)          ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAg                                                                                                                             <  6:124497/148‑1 (MQ=255)              cccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGc                                                                                                                        <  7:10920/149‑1 (MQ=255)               ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTgg                                                                                                                         >  2:315287/1‑147 (MQ=255)                 gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAg                                                                                                                      >  4:255807/1‑148 (MQ=255)                 gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAg                                                                                                                      >  2:154532/1‑148 (MQ=255)                 gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGCCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCCGTTTTCACCTGGCAg                                                                                                                      >  6:49482/1‑148 (MQ=37)                     gggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAAt                                                                                                                  >  8:488/1‑148 (MQ=255)                        cTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACt                                                                                                              >  3:42309/1‑149 (MQ=255)                          gATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc                                                                                                            >  2:117520/1‑149 (MQ=255)                            tAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTg                                                                                                          >  4:399340/1‑149 (MQ=255)                              gAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGc                                                                                                         <  5:330550/148‑1 (MQ=255)                               aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGCGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                       >  1:80421/1‑149 (MQ=255)                                aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTc                                                                                                      <  7:69902/149‑1 (MQ=255)                                    ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcaca                                                                                                   <  3:399340/148‑1 (MQ=255)                                    ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCccac                                                                                                  >  3:87471/1‑149 (MQ=255)                                     tgatagggCTGCGAGGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACAcc                                                                                                 >  7:387352/6‑149 (MQ=37)                                       aGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt                                                                                               <  3:255807/149‑1 (MQ=255)                                        gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                              >  2:393746/1‑149 (MQ=255)                                        gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                              <  3:212828/149‑1 (MQ=255)                                                ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTTCAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACtt                                                                                                             >  7:55644/1‑126 (MQ=255)                                                ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACtt                                                                                                             <  8:55644/126‑1 (MQ=255)                                                ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACt                                                                                        <  5:159318/147‑1 (MQ=255)                                                ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                       <  1:154532/148‑1 (MQ=255)                                                    gCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGatat                                                                                   <  6:422127/148‑1 (MQ=255)                                                       gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                               <  6:324336/149‑1 (MQ=255)                                                       gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                               <  1:393746/149‑1 (MQ=255)                                                       gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                               <  8:387352/149‑1 (MQ=255)                                                        cccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTTCTTCTGCTTCACACCTTCGACACTGATAtccagg                                                                              >  5:55495/1‑149 (MQ=255)                                                               aCGACACAAGGCCGCTCCTGCATAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                        <  3:161894/148‑1 (MQ=255)                                                                 gACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                                      <  1:282042/148‑1 (MQ=255)                                                                 gACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                                      >  2:282042/1‑148 (MQ=255)                                                                    acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                  <  7:328027/149‑1 (MQ=255)                                                                     caAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg                                                                 <  8:290442/149‑1 (MQ=255)                                                                       aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGaa                                                               <  4:42309/149‑1 (MQ=255)                                                                        gcgcGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGTCCTTGTGTCGTGAT‑AGTGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCAGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAg                                                              <  4:105189/146‑1 (MQ=255)                                                                         gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAg                                                              >  4:139815/1‑148 (MQ=255)                                                                          ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTGACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                            <  1:315287/149‑1 (MQ=255)                                                                                   cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCggg                                                   >  4:40067/1‑149 (MQ=255)                                                                                    agagAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAAGGGCCGCAAAGC‑GACCCCCTCGGTTTTCAGATGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCAAGGTCCAGGGTCTGCGAAGTAGCACCCggg                                                   >  1:315464/1‑149 (MQ=255)                                                                                    agagAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGc                                                  <  7:488/149‑1 (MQ=255)                                                                                      agaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCt                                                >  5:65652/1‑149 (MQ=255)                                                                                        aaTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt                                              >  3:431110/1‑149 (MQ=255)                                                                                         aTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt                                              <  4:359248/148‑1 (MQ=255)                                                                                         aTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt                                              <  6:65652/148‑1 (MQ=255)                                                                                          tttCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGa                                            >  3:60140/1‑149 (MQ=255)                                                                                               tGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGC‑GTCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGTTCTGCTAACTAGCACCACGGCCTTTGATGcc                                        >  7:210933/1‑148 (MQ=38)                                                                                                 aTCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCgg                                                    >  2:317132/1‑134 (MQ=255)                                                                                                 aTCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCgg                                                    <  1:317132/134‑1 (MQ=255)                                                                                                 aTCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                        >  7:299974/1‑146 (MQ=255)                                                                                                 aTCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                        <  8:299974/146‑1 (MQ=255)                                                                                                  tctcTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAg                                    >  1:192495/1‑149 (MQ=255)                                                                                                      tACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                              >  1:205864/1‑87 (MQ=37)                                                                                                      tACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                              <  2:205864/87‑1 (MQ=37)                                                                                                      tACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                     <  4:87471/144‑1 (MQ=255)                                                                                                      tACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGt                                   <  5:359392/146‑1 (MQ=255)                                                                                                        cAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                               >  3:353627/1‑148 (MQ=255)                                                                                                        cAGGAACGGCCATCTCTCGTGAT‑AGGGCCGCAAAGCAG‑ACCCCACGCAATTCAGCTGGCAGAAATAACTTCAGCTTCACACCTACCAAACACAAAACCAGTTCCACGTTATTCGAAGTATCACCCGTCACTATCATGCCGAATTAgtt                               >  8:74731/1‑148 (MQ=255)                                                                                                           gAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                           >  1:415215/1‑149 (MQ=255)                                                                                                                gCCTTGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttcag                      >  3:270017/1‑149 (MQ=255)                                                                                                                   ttGTGTCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt                   >  4:153574/1‑149 (MQ=255)                                                                                                                     gtgtCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAGTTATGCTTCCCACCTTCGACACTGTTATCAAGGTCCAGGGTCTGCGAAGTAGCCCCCGGGCGTTGGATGCGGAAGGCGTTCTGGTTCAGCgtgt                 >  6:256769/1‑148 (MQ=255)                                                                                                                        tCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttca                       >  6:280683/1‑140 (MQ=255)                                                                                                                        tCGTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttca                       <  5:280683/140‑1 (MQ=255)                                                                                                                          gTGAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg            <  5:49482/149‑1 (MQ=255)                                                                                                                            gAT‑AGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGc          >  3:268719/1‑149 (MQ=255)                                                                                                                                aGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt       >  7:393808/1‑149 (MQ=255)                                                                                                                                  ggCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      >  8:272121/1‑148 (MQ=255)                                                                                                                                  ggCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      >  1:230856/1‑148 (MQ=255)                                                                                                                                   gCCGCAAAGCGG‑CCCCGTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa    <  3:40067/149‑1 (MQ=255)                                                                                                                                   gCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      >  4:224691/1‑147 (MQ=255)                                                                                                                                   gCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa    <  4:431110/149‑1 (MQ=255)                                                                                                                                   gCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa    <  2:80421/149‑1 (MQ=255)                                                                                                                                   gCCGCAAAGC‑GACCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa    <  2:315464/149‑1 (MQ=255)                                                                                                                                     cGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc  >  7:282203/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                          CCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAT‑AGGGCCGCAAAGC‑G‑CGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674612‑5674890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑