Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,986,861	C→T	intergenic (+141/‑58)	PP_5245 → / → kefB‑III	AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,986,861	0	C	T	100.0%	218.3 / NA	64	intergenic (+141/‑58)	PP_5245/kefB‑III	AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/31);  total (33/31)

GCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGCCAAGCGCCTGC  >  NC_002947/5986720‑5987003                                                                                                                                              |                                                                                                                                                 gcgcTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTg                                                                                                                                           >  2:6467/1‑149 (MQ=255)   gcTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTc                                                                                                                                         >  4:334635/1‑149 (MQ=255)    cTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTcc                                                                                                                                        <  7:209631/149‑1 (MQ=255)          tcttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCaaa                                                                                                                                  >  6:182700/1‑149 (MQ=255)           cttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGAGAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCaaa                                                                                                                                  <  3:315225/148‑1 (MQ=255)           cttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCaaa                                                                                                                                  <  4:165860/148‑1 (MQ=255)           cttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCaaa                                                                                                                                  <  1:13253/148‑1 (MQ=255)              cgcgGGCGCGCCCGCTCCCTCAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTCGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAAc                                                                                                                                 <  5:301150/146‑1 (MQ=255)               gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGtt                                                                                                                             >  8:229941/1‑149 (MQ=255)               gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGtt                                                                                                                             <  7:47924/149‑1 (MQ=255)                   gcgcgcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGaa                                                                                                                         <  1:63806/149‑1 (MQ=255)                     gcgcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACg                                                                                                                       >  1:235553/1‑149 (MQ=255)                        cccGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTg                                                                                                                    <  2:235553/149‑1 (MQ=255)                         ccGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGc                                                                                                                   >  7:205618/1‑149 (MQ=255)                               ccACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg                                                                                                              >  1:445965/1‑148 (MQ=255)                               ccACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGc                                                                                                             <  7:382296/149‑1 (MQ=255)                                       aTAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg                                                                                                     <  6:39337/149‑1 (MQ=255)                                       aTAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg                                                                                                     >  5:270569/1‑149 (MQ=255)                                          gCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa                                                                                                   <  3:334635/148‑1 (MQ=255)                                           cacaGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGt                                                                                                 <  1:6467/149‑1 (MQ=255)                                            acaGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAAtt                                                                                                                                            <  6:136093/105‑1 (MQ=255)                                            acaGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAAtt                                                                                                                                            >  5:136093/1‑105 (MQ=255)                                             caGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTAc                                                                                               <  6:270569/149‑1 (MQ=255)                                                   ttGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGcc                                                                                          >  3:396362/1‑148 (MQ=255)                                                        cccAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGc                                                                                     >  2:255086/1‑148 (MQ=255)                                                           aGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCcaca                                                                                 <  5:182700/149‑1 (MQ=255)                                                               aaGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACg                                                                               >  6:12074/1‑147 (MQ=255)                                                                aGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGaa                                                                             <  2:413164/148‑1 (MQ=255)                                                                aGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAg                                                                            <  2:445965/149‑1 (MQ=255)                                                                     cGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGc                                                                       >  5:353280/1‑149 (MQ=255)                                                                       gCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCt                                                                     >  4:216307/1‑149 (MQ=255)                                                                        cACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCtt                                                                    <  8:238492/149‑1 (MQ=255)                                                                         aCCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTc                                                                   >  2:357677/1‑149 (MQ=255)                                                                         aCCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTc                                                                   <  4:396362/149‑1 (MQ=255)                                                                           cGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑g                                                               >  6:302475/1‑149 (MQ=255)                                                                              ttGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCa                                                             >  6:267375/1‑148 (MQ=255)                                                                                  cACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACACTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACcg                                                        >  7:172789/1‑149 (MQ=255)                                                                                   aCTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACcgc                                                       <  7:229941/149‑1 (MQ=255)                                                                                       tGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCTCACGAAGGCAGCCTTCT‑‑GCAAACCGCgg                                                     <  1:255086/147‑1 (MQ=255)                                                                                        ggacaggacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCACTTAGAATTGTCAGTCAAACTGTTCGAACGCTCCCAAGGCAGCTCATGGAGTACGAGCCAATGACAACCGAAGGAAGCCATCT‑‑GCAAACcccg                                                      >  5:262075/1‑145 (MQ=255)                                                                                              gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATc                                                >  6:150750/1‑145 (MQ=255)                                                                                              gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATc                                                <  5:150750/145‑1 (MQ=255)                                                                                              gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTcc                                            >  6:127449/1‑149 (MQ=255)                                                                                              gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTcc                                            >  8:129634/1‑149 (MQ=255)                                                                                              gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTcc                                            >  8:277083/1‑149 (MQ=255)                                                                                               acaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGt                                                    <  7:205450/140‑1 (MQ=255)                                                                                               acaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGt                                                    >  8:205450/1‑140 (MQ=255)                                                                                               acaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCt                                           <  8:205618/149‑1 (MQ=255)                                                                                                caATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTccag                                          >  5:57334/1‑147 (MQ=255)                                                                                                   tttttCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCtt                                       <  7:277083/149‑1 (MQ=255)                                                                                                         ttcCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTgccgcc                                 >  2:174788/1‑149 (MQ=255)                                                                                                          tcacGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTgccgcc                                 >  4:341774/4‑148 (MQ=255)                                                                                                             cctCAGATGATTGACTGACGCCACACTACCCCTTACAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCAACACGAAGGCACCCTACT‑‑GCAAAACGCGGTGATATTACTGCTTGCCGCCTTc                              >  6:171530/3‑148 (MQ=255)                                                                                                                 aGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGc                         >  8:262398/1‑149 (MQ=255)                                                                                                                     gatTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTc                     >  6:280046/1‑149 (MQ=255)                                                                                                                       ttGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTccc                   >  2:442381/1‑149 (MQ=255)                                                                                                                          tCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCt                >  5:199769/1‑149 (MQ=255)                                                                                                                          tCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCt                <  1:442381/149‑1 (MQ=255)                                                                                                                          tCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCt                <  5:280046/149‑1 (MQ=255)                                                                                                                            tGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTgg              >  8:378622/1‑149 (MQ=255)                                                                                                                              actccACACTAGCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCATCATTCT‑‑TCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTgtc             <  5:171530/145‑3 (MQ=255)                                                                                                                              aCGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGcc            <  3:216307/149‑1 (MQ=255)                                                                                                                                         ccccTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGT‑CCCCTGGCCAAGCGCCTGc  <  1:357677/149‑1 (MQ=255)                                                                                                                                         ccccTTAGAATTCTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGCCAAGCGCCTGc  <  3:341774/148‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                 GCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCT‑‑GCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGCCAAGCGCCTGC  >  NC_002947/5986720‑5987003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑