Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,499,498 1 bp→CG intergenic (+75/‑89) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,499,4971.C93.0% 118.4 / 3.4 46intergenic (+74/‑90)trpS/zapEtryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base C (20/20);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0029471,499,4980TG97.8% 133.6 / NA 46intergenic (+75/‑89)trpS/zapEtryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (22/23);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCG‑GTGT‑T‑T‑CGGGCA‑CG‑CCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCG  >  NC_002947/1499351‑1499646
                                                                                                                                                      |||                                                                                                                                                      
gTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGGGCCCGCGCAAAACGCGCCGCGGTGCCTGGCACCGGCGTTGGCG‑GGGt‑t‑tg                                                                                                                                                         >  3:184720/1‑148 (MQ=255)
         cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑cg‑c                                                                                                                                              <  6:227143/149‑1 (MQ=37)
           cTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCCCGGTGCATGGCACCCGCATTGCCG‑GTGT‑TCG‑CGGGCA‑cg‑ccc                                                                                                                                            >  6:232215/1‑148 (MQ=37)
               aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑cg‑cccgctc                                                                                                                                        <  6:143446/149‑6 (MQ=25)
                                 tCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑At                                                                                                                      >  5:89525/1‑149 (MQ=21)
                                        cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCgg                                                                                                               <  7:235043/149‑1 (MQ=21)
                                         tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGGCCGCGAAACAGGCGCCGCCGGGCATGGGACCGGGGTTGCCG‑GTGT‑T‑TGCGGGGACCG‑CCGCGCGCACACACGGAGTGGC‑AGTTAGgggg                                                                                                              >  8:184573/1‑149 (MQ=255)
                                           aaCGAAGCCTTGGCACCCCAGCAGGACTGAGGCTGCCAAACGGTGGGTGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGGTTTGCCGCGTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCgg                                                                                                               <  1:328421/147‑1 (MQ=21)
                                            aCGAAGCCTTGTCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCt                                                                                                           <  1:154173/149‑1 (MQ=21)
                                            aCGAAGCCTGGCCACCACCGCCGCACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAACCGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCt                                                                                                           <  3:214173/149‑1 (MQ=18)
                                                  ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCCCAAAACGCGCCCCGGGGCATGGCACCCGCGTTTCCC‑GTGT‑TAG‑CGGGGA‑CGCCCGCACCCCCAAACAGAATTGC‑AATAAACCGGCCGATCGc                                                                                                      >  4:156209/1‑148 (MQ=255)
                                                   cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGTCGCCGCTGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGc                                                                                                             <  5:317120/140‑1 (MQ=21)
                                                          ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCCGCTTTGCCG‑GTGT‑TAG‑CGGGCA‑CGCCCGCGCCCCCAGCCTGATTGCC‑ATTAATCGGGCCGATCGCTACAGTGAc                                                                                             >  2:294059/1‑149 (MQ=21)
                                                             ctgcagGACGGCGGCTGCCAAACGGTGGGAGCGGGCGGGCCCGCGAAACCGGCGCCGCGGGGCATGGCACCGGCTTTGCCG‑GTGTGG‑G‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGc                                                                                          <  4:317636/147‑1 (MQ=21)
                                                              agcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAACGCGCCGCGGTGCAAGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGGC‑CGCCCGCGCCCACAGCCTGAATGGC‑ATTAAGCGGGGCGATCGCTAAAGTGAAGGcc                                                                                         >  2:129224/1‑149 (MQ=12)
                                                               gcagGACTGCGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGGGGTGCATGGCACGGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                                                        <  2:148559/149‑1 (MQ=21)
                                                                        aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCCCAGACTGATTGCC‑ATTAAGCGGGCCTATCGCGACAGTGACGGCCCGTGTTttgg                                                                               >  8:296967/1‑148 (MQ=21)
                                                                        aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt                                                                                 <  8:210883/147‑1 (MQ=21)
                                                                         ggcggcCAAACGGTGGTCGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCCG‑GTGTGC‑G‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                              <  3:174555/149‑1 (MQ=25)
                                                                               ctatcGGTGGGAGCGGGGGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCg                                                                        <  1:408250/145‑1 (MQ=21)
                                                                                   cGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa                                                                    <  2:28336/149‑1 (MQ=21)
                                                                                        gtaGCGGGCGGGCCCGCTAAACTGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑GGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt                                                               <  8:354884/147‑1 (MQ=21)
                                                                                          aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TGG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                                                             <  5:232215/149‑1 (MQ=25)
                                                                                           gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                            <  5:341876/149‑1 (MQ=25)
                                                                                            cGGGGGTGCCCGCTAAACAGGGGCCGCGGTGCATGGCACCGGCTTTTCCG‑GTGT‑TCG‑CGGTAA‑CGCCCGCTCCCAAAGACTGATTGCCTCTTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                            <  1:129224/149‑1 (MQ=21)
                                                                                             gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                                                          <  3:156209/149‑1 (MQ=37)
                                                                                                    cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                                    <  6:89525/148‑1 (MQ=37)
                                                                                                     ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                                    >  2:146626/1‑147 (MQ=37)
                                                                                                     ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                                    >  3:157894/1‑147 (MQ=37)
                                                                                                      cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑CTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTCACGGCCCGTGTTTAGTTGCCTCGATAACGAATAATGACTCCCt                                                    >  4:317578/1‑146 (MQ=21)
                                                                                                       gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg                                                 >  3:250945/1‑148 (MQ=37)
                                                                                                       gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg                                                 >  3:364154/1‑148 (MQ=37)
                                                                                                          aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                  <  5:428203/144‑1 (MQ=37)
                                                                                                          aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                  >  6:428203/1‑144 (MQ=37)
                                                                                                           aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc                                            >  5:197704/1‑149 (MQ=37)
                                                                                                            aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc                                            >  4:394060/1‑148 (MQ=37)
                                                                                                             cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa                                          >  2:270427/1‑149 (MQ=37)
                                                                                                              aGGCGCCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa                                          >  4:15621/1‑148 (MQ=37)
                                                                                                                gcgcCGCGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc                                        >  4:285019/1‑148 (MQ=37)
                                                                                                                  gccgcGGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg                                     <  4:250945/149‑1 (MQ=37)
                                                                                                                    cgcgGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg                                     >  7:48621/1‑147 (MQ=37)
                                                                                                                    cgcgGTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCa                                   <  4:157894/149‑1 (MQ=37)
                                                                                                                        gTGCATGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATc                               <  1:294059/149‑1 (MQ=38)
                                                                                                                            aTGGCACCGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGCGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGa                            >  6:18362/1‑148 (MQ=37)
                                                                                                                             tggcaccGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaaa                          >  3:446222/7‑149 (MQ=38)
                                                                                                                              ggcaccGGCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAAc                         >  2:30063/6‑149 (MQ=38)
                                                                                                                                     gCTTTGCCG‑GTGT‑TCG‑CGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGa                  >  3:414673/1‑149 (MQ=39)
                                                                                                                                                       gcGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgc   >  2:230210/2‑149 (MQ=255)
                                                                                                                                                       gcGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcg    <  4:446222/147‑1 (MQ=255)
                                                                                                                                                        cGGGCA‑CGCCCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgcg  >  6:170751/1‑149 (MQ=255)
                                                                                                                                                      |||                                                                                                                                                      
GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCG‑GTGT‑T‑T‑CGGGCA‑CG‑CCGCTCCCACAGACTGATTGCC‑ATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCG  >  NC_002947/1499351‑1499646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑