Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,740,819 2 bp→CT intergenic (+56/+696) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8190TC93.5% 120.3 / ‑5.7 47intergenic (+56/+697)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base C (21/22);  minor base A (2/1);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0029474,740,8200GT95.7% 155.1 / ‑4.3 47intergenic (+57/+696)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (23/22);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCG  >  NC_002947/4740677‑4740957
                                                                                                                                                     ||                                                                                                                                          
tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                      >  2:140979/1‑149 (MQ=255)
  cAGCCCGTACAAGATCGTGCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                      <  5:36529/147‑1 (MQ=255)
  cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGcc                                                                                                                                       <  4:332227/146‑1 (MQ=255)
  cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                                    <  4:383447/149‑1 (MQ=255)
      ccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                                <  4:423184/149‑1 (MQ=255)
        gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGTACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc                                                                                                                              <  1:125311/149‑1 (MQ=255)
         tACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAAAGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCttag                                                                                                                             >  3:224601/1‑147 (MQ=255)
            aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg                                                                                                                          >  5:253463/1‑149 (MQ=255)
            aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg                                                                                                                          >  5:148251/1‑149 (MQ=255)
                 cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGc                                                                                                                         >  4:22933/1‑145 (MQ=255)
                 cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGc                                                                                                                         <  3:22933/145‑1 (MQ=255)
                   gCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGcc                                                                                                                   >  2:369354/1‑149 (MQ=255)
                          gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGTGT‑‑AGCGAACGCAAG‑‑‑AAGGCAGCCCAAGGGCAGCC‑GTTTTTATTTTTACTGTTCCGGCCTAATAGCGGCCGTTGCCGCGa                                                                                                              >  1:367890/1‑148 (MQ=255)
                                    tACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCt                                                                                                                       >  8:275843/1‑128 (MQ=255)
                                    tACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCt                                                                                                                       <  7:275843/128‑1 (MQ=255)
                                      cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTATGCTGCAGT‑ACGGCCGAACGCAAGCTTGCGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCgg                                                                                                     <  1:140979/149‑1 (MQ=255)
                                      cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGca                                                                                                   >  7:101523/1‑146 (MQ=255)
                                       acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAgg                                                                                                         <  3:307294/139‑1 (MQ=255)
                                       acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAgg                                                                                                         >  4:307294/1‑139 (MQ=255)
                                       acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAgc                                                                                               >  2:335284/1‑148 (MQ=255)
                                         cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGGACCCCAAGTAACCTGAAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCACGGGCAGCC‑TTTTTTATTTTTCATGTCCCGGCATATTCCCGGCCTTGCCCGCGAAGAGGCCGGCAAAgc                                                                                               >  1:297720/1‑147 (MQ=255)
                                         cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCGCCCTTGCCCGCGAAGAGGCCGGCACAgccc                                                                                             >  6:382129/1‑146 (MQ=255)
                                             atctaGAAGGGCGACTTCTTTGCCGTGAAT‑GACCCCATGTACGCTGAAGT‑T‑‑GCCGACCGCAAG‑‑‑ATGGCGGCCCTGGGGCAG‑CTTTTTTTATTTGTCCTGTCCGGGCCTGTTCGCGGCCTTGCCGGGTATGAGGCCGGCACAGAca‑cca                                                                                         <  8:243653/144‑1 (MQ=255)
                                                  gAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGCAGCGGCCGGCACAGAca‑cca                                                                                         >  7:243653/1‑143 (MQ=255)
                                                       gcgACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAg                                                                              <  7:191336/149‑1 (MQ=255)
                                                        cgACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGt                                                                             >  5:92974/1‑149 (MQ=255)
                                                              cGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATAGCGGCCATGCCCCCGCAGAGGCCGGCACAGACA‑CCACAAAACCACAGTTTTtc                                                                        >  4:195304/1‑148 (MQ=255)
                                                                     cTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGCGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTccgcccg                                                                >  8:232855/1‑149 (MQ=255)
                                                                      tGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCGGCCGTGCCCGCGCAGAGGCCGGCCCAGGCA‑CCCCAAAACCGCAGTTTTTCTccgcccg                                                                >  3:409892/1‑148 (MQ=255)
                                                                       gAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGCGGCCGGCCCCGCCA‑CCACAAAACCGCAGTGTTTGTCCGCCCGCt                                                              >  8:326107/1‑149 (MQ=255)
                                                                            gACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                          <  6:148251/149‑1 (MQ=255)
                                                                              ccGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCCCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCa                                                        <  1:369354/149‑1 (MQ=255)
                                                                                  aaGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACCGACA‑CCACAAAACCGCAGTTTTTATCCGCCCGCTCACGCAGccc                                                    >  5:412507/1‑149 (MQ=255)
                                                                                   ggTACGCTGCAGT‑T‑‑GCCCAACGGACG‑‑‑AAGGGTGCCCTCGGGCAGCGTTTTTTT‑TTTGTCAGGTGCCGGCCTCTGGGGGGGCTTGCCCGCGAAGTGGCCGGCACAGACA‑CAACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCt                                                   <  6:324735/148‑1 (MQ=255)
                                                                                    gTACGCTCCAGT‑C‑‑GCCGAACCCACG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAATAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                  <  2:260364/149‑1 (MQ=255)
                                                                                         cTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                             <  2:367890/149‑1 (MQ=255)
                                                                                         cTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                             <  3:361542/149‑1 (MQ=255)
                                                                                            atgT‑A‑‑GCCCAACCCAAG‑‑‑ACGGCTGCCCGCGGCCAGCTTTTTTTTTTTGCCCTGG‑CCCGGCCGCTGCGCGGCTTTCCCCGCTAAGCGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTa                                          <  3:195304/147‑1 (MQ=255)
                                                                                                 a‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCGGCCTTGCCCGCGAAGAGGCCCGCACAGACA‑CCCCAAAACCACAGTTTTTATACGCCCGCTCACGCAGCCCGTTCAGGGTATTg                                       >  2:2192/1‑148 (MQ=255)
                                                                                                 a‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGACAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAtt                                        <  1:341000/148‑1 (MQ=255)
                                                                                                             ataaaaaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCATGTGCCGGCCAATTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAACCCTCAGTTTTTATACCCCCGATCACGGACCCGTTTCAGGGTATTGAACGgcg                                >  4:329487/7‑148 (MQ=255)
                                                                                                              gcaaccagGCGGCCCGCGGGCAG‑CTTTTTTTTTTTGCCCTGTGCCGGCCTCTGCGCGCCGTGGCCCGCTATGTGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTTAGGGTATTGAACGGCGCa                              <  4:224601/143‑1 (MQ=255)
                                                                                                                    cggcggcCCTCGGCAAGCC‑TTTTTTATTTGCCCTGGGCCGCCCTCTTCGCGGCCTTCCCCGCTAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacc                        <  1:233878/148‑1 (MQ=255)
                                                                                                                          ccgtCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAc                  <  2:297720/146‑1 (MQ=255)
                                                                                                                               gtgCAGCC‑TTTTTTATTTGTCCTGTGCGGGCCGCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt             <  2:336685/147‑1 (MQ=255)
                                                                                                                               gggAAGCC‑TTTTTTATTTTTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAATAGGCCGGCACAGACA‑ACACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt             <  6:92974/149‑1 (MQ=255)
                                                                                                                                           tttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCg  >  7:312783/1‑149 (MQ=255)
                                                                                                                                                     ||                                                                                                                                          
TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAA‑GACCGCAAGTAAGCTGCAGT‑A‑‑GCCGAACGCAAG‑‑‑AAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACA‑CCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCG  >  NC_002947/4740677‑4740957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑