Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 3,845,705 +G intergenic (‑72/+26) PP_3394 ← / ← PP_3395 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,845,7031.G92.2% 151.2 / 7.5 51intergenic (‑70/+28)PP_3394/PP_33953‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base G (33/14);  total (34/17)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑G‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGC  >  NC_002947/3845557‑3845842
                                                                                                                                                    |                                                                                                                                            
gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTc‑gc‑g                                                                                                                                             <  4:156581/148‑1 (MQ=255)
gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑g‑g                                                                                                                                           <  2:232762/149‑1 (MQ=255)
 ctgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑g‑gg                                                                                                                                          <  3:286373/149‑2 (MQ=255)
       tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATc                                                                                                                                     >  3:188099/1‑149 (MQ=255)
         ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATcc                                                                                                                                    >  1:175100/1‑148 (MQ=255)
         ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATcc                                                                                                                                    <  2:175100/148‑1 (MQ=255)
            aTGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCg                                                                                                                                >  7:374542/1‑149 (MQ=255)
              gATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGtg                                                                                                                              >  8:213387/1‑148 (MQ=255)
              gATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGt                                                                                                                               >  3:378156/1‑148 (MQ=255)
                    ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGtggctgac                                                                                                                        >  6:197750/1‑142 (MQ=255)
                    ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCAc                                                                                                                        >  3:388616/1‑149 (MQ=255)
                      gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGa                                                                                                                      >  2:27914/1‑149 (MQ=255)
                       cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGAt                                                                                                                     <  7:197152/149‑1 (MQ=255)
                         ggCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATgg                                                                                                                   <  7:79342/149‑1 (MQ=255)
                            tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGAt                                                                                                                 >  6:369713/1‑148 (MQ=255)
                              tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAg                                                                                                              >  3:245080/1‑149 (MQ=255)
                              tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTCGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAg                                                                                                              >  7:185849/1‑149 (MQ=255)
                               gCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGc                                                                                                             >  4:90858/1‑149 (MQ=255)
                                cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTGGCTGACGATGGATCa                                                                                                               <  8:374542/146‑1 (MQ=255)
                                 aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGccc                                                                                                           >  6:213317/1‑149 (MQ=255)
                                  aGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGAGGGATCAGCCcg                                                                                                          >  6:312101/1‑149 (MQ=255)
                                  aGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GAGCTGATCCATCGTCAGCCACGATGGAt                                                                                                                 >  3:13756/1‑142 (MQ=255)
                                  aGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GAGCTGATCCATCGTCAGCCACGATGGAt                                                                                                                 <  4:13756/142‑1 (MQ=255)
                                   gCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTGGCTGACGATGGATCAGCCcgc                                                                                                         <  5:197750/149‑1 (MQ=38)
                                   gCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCa                                                                                                               >  5:361939/1‑143 (MQ=255)
                                   gCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCa                                                                                                               <  6:361939/143‑1 (MQ=255)
                                     cATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCgcgc                                                                                                       >  5:302170/1‑149 (MQ=255)
                                           gcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGc                                                                                                  >  5:416204/1‑148 (MQ=255)
                                            cAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCa                                                                                                <  5:369713/149‑1 (MQ=255)
                                                ccGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGcc                                                                                            >  5:108448/1‑149 (MQ=255)
                                                 cGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAAACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGCTCCATAGTCAGCCACGATGGATAAGCCCCCCCCCTGCCCGGCCg                                                                                           >  6:49137/1‑149 (MQ=255)
                                                 cGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGAGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCg                                                                                           >  1:319564/1‑149 (MQ=255)
                                                        attggttgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACg                                                                                    >  7:1706/1‑149 (MQ=255)
                                                        attgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACg                                                                                    >  7:328011/1‑149 (MQ=255)
                                                                ttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCaa                                                                              >  3:206092/1‑147 (MQ=255)
                                                                   ttgttgGCATCCCGGGCGCTCGGTCTGACGCGTGGCGCCAGACTACGCGCGGGAGGCCGGGCAGAAAAGCCGTGTTTC‑GCGG‑GCTCATCCATCGCCCGCCACGAGGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg                                                                          <  6:416204/148‑1 (MQ=255)
                                                                      ttgtCTTCGCGTGCTATTTGTCTGAAGCGTTGCGCCAGACTATTTGGTGGTTGAGGGGCATAAATGACGTTTTTCGGG‑G‑GCTTATCTTTGGTCAGCCACGATGTATAAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtgg                                                                       <  6:108448/148‑1 (MQ=255)
                                                                           aTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGCCGGGCAGAAAACACGTGTTGC‑GCGG‑GCTGATCCATCGTCACCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGc                                                                 <  3:90858/149‑1 (MQ=255)
                                                                              gcgGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAgg                                                              <  1:27914/149‑1 (MQ=255)
                                                                                    tctctGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgcc                                                          >  1:226394/1‑147 (MQ=255)
                                                                                      tctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagc                                                      >  1:163554/1‑149 (MQ=255)
                                                                                          tctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGAc                                                  <  1:112430/149‑1 (MQ=255)
                                                                                            tgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAg                                                >  3:200600/1‑149 (MQ=255)
                                                                                            tgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAg                                                >  3:162833/1‑149 (MQ=255)
                                                                                              aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATACATCGTCAGCCACGATGGGTCAGCCCGCCCCCTGCCAGGCCGGTGCACGCACCAAAAGGTGGTCAAGCAGGCGCCGCGCCCACAGc                                               >  7:146271/1‑148 (MQ=255)
                                                                                                       gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtgt                                     <  5:213317/149‑1 (MQ=255)
                                                                                                          aGACTAAGAGCTGGATGACGGGAAGAACACACGTGTTGCGGG‑G‑GCTGTTCCATGGTCCGCCACGTGGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGGCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGcc                                  <  5:312101/149‑1 (MQ=255)
                                                                                                           gACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCg                                 >  5:320379/1‑149 (MQ=255)
                                                                                                              tAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTc                              >  5:315970/1‑149 (MQ=255)
                                                                                                                agagCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcg                             <  8:328011/148‑1 (MQ=255)
                                                                                                                          gACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGAt                  >  2:303722/1‑149 (MQ=255)
                                                                                                                            cGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGa                <  2:319564/149‑1 (MQ=255)
                                                                                                                             gggCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc               >  5:388558/1‑149 (MQ=255)
                                                                                                                                          gtgtTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgc  >  5:171262/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑G‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGC  >  NC_002947/3845557‑3845842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑