| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,126,647 | +C | intergenic (‑101/+48) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 92.6% | 161.6 / 7.2 | 54 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (4/0); new base C (18/32); total (22/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGC > NC_002947/1126498‑1126782 | tGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCCCGAATGGGGAGGTATAGCCACCAAAGCAATAGC‑GGG‑G‑aaaa > 2:97164/1‑148 (MQ=255) gggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATTCGGAGGTATAGCCCCCATAGCATTAGC‑GGG‑T‑AAA‑cccgca > 8:393410/1‑145 (MQ=255) gggCCGATGCCGATCTTGAACAGGTCGAAGACGATCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCGCGAATGCGGAGGTTTAGCCACCATCGCAATAGC‑GGG‑T‑TAA‑caccct > 1:55355/1‑144 (MQ=255) ggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑cccgctc < 1:161195/149‑6 (MQ=255) gatgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTac < 5:386724/149‑1 (MQ=255) atCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCCAATGCGGAGGTATAGGCACCAAAGCATTAGC‑CGG‑TAAAA‑CCCCTCCAACACAGAGc > 6:195104/1‑149 (MQ=255) cTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCGAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCGCGAATGCGGGGGTATAGCCACCATAGCATTAGC‑GGGTT‑AAG‑CCGCTGCGACACAGAGccc > 5:186225/1‑149 (MQ=255) aCAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCAc < 7:15822/149‑1 (MQ=255) gAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGCTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGc > 3:356798/1‑148 (MQ=255) aGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCTAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAg < 2:366738/149‑1 (MQ=255) aGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAg < 5:195104/149‑1 (MQ=255) gACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGCTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAgg < 4:356798/149‑1 (MQ=255) aCGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCTTTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGt < 1:97164/149‑1 (MQ=255) aGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATTGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCg < 1:123718/147‑1 (MQ=255) aGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCg < 4:331766/147‑1 (MQ=255) tGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGa < 4:306157/149‑1 (MQ=255) aCAGGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAg < 7:171911/148‑1 (MQ=255) aTGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATGCGGAGGTATAGCCCCCATAGCATTAGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCCCCACCCAACCCAGGTGGCGGGGAggg > 6:218812/1‑148 (MQ=38) aTGTGGACCTCCTGCCAGGGTGTGGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCAGCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGc < 7:393410/149‑1 (MQ=38) aCCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCCAATGCGGAGGGATAGCCACCATCGCAATAGC‑GGG‑T‑AAACCCGCACCTACACAGATCCCCCCAACCACACCAGGGGGCGGGGAGGGCTATCAg > 3:82657/1‑149 (MQ=255) cctcgtGCCAGGTTTTTGGGTGTACATTTTGCCTTTTAGGCGGGGGTTTACCGGCGAATGGGTAGTTATAGCAACAATCGAATTCGC‑GGT‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg < 8:58498/148‑1 (MQ=255) cctcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTAGC‑GGG‑G‑AAACCCCCTCCTACACAGAGCCCGCCCCCCAACCCACGGGGCGGGGTCGGCTAtaatg > 7:125115/1‑145 (MQ=37) cctcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCCCGAATGCGGAGGTATAGCCACCATCGCATTAGC‑GGG‑TAAAA‑CCCCTCCGACACAGAGCCCGCCACCCAACCCACGTGGCCGGGCGGGCTAACAgg > 5:326140/1‑149 (MQ=255) ctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGt < 7:97757/148‑1 (MQ=255) ctGCCAGGTTGGTGGGTGGACATGGAGCCCTGTAGGCGCGGGTTAACCCGCGAATGCGCAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCg < 2:370429/147‑1 (MQ=38) ccAGTTTGTTGGGTGCACATTGAGCCCTGTAGGCGCGGTTTTACCCGCGAATGCGGATGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTcc < 3:201301/147‑1 (MQ=38) aGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCGCCAATTCGGCGGTATAGCCCCCATCGCATTAGC‑GGG‑T‑AAACCCGCACCTACACAGACCCCGCCACCCAACCCAGGTGGCGGGGAGGGCTATAAGGCGtgcaga > 2:47675/1‑143 (MQ=255) aGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa < 1:47675/148‑1 (MQ=255) gttgGGTGGACATTGAGCCCTGTAGGGGCGGGTTTACGCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGACGCCTGATAGcc < 6:186225/148‑2 (MQ=37) tGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcg‑atc < 2:55355/149‑1 (MQ=255) aTTGAGCCCTGTAGGCGCGGGTTTAACCGCCAATTCGGATGTATTTCCCCCATACCCTTATCGGGG‑A‑AAA‑CCCCGACAAAACACAGCCCCCCCCCCCAACCACGGGGCGGGGAGGGCTATAAAGCCTCCTGATATATCACC‑ATCGAccg > 6:343694/1‑147 (MQ=255) gTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTAGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGca > 6:208827/1‑148 (MQ=255) ttcgcgGGTAAACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcg‑a > 3:273037/3‑129 (MQ=38) ttcgcgGGTAAACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcg‑a < 4:273037/127‑1 (MQ=38) gcgcGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCa < 5:208827/149‑1 (MQ=255) tttACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCg < 7:192861/149‑1 (MQ=255) ttACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCg < 4:82657/148‑1 (MQ=255) ccGCGAAGGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc < 6:326140/149‑1 (MQ=255) ttcGGAGGTATTGCCACCATCGCATTCGG‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCGACCCATGGCAGTTGGCGGGGAGGTCTATCAg < 4:351636/88‑1 (MQ=21) tGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg > 3:351636/1‑90 (MQ=255) ggAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGGTGCGGTCGCCGAACACGtt > 4:46327/1‑149 (MQ=255) aGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGt > 8:17095/1‑149 (MQ=255) aGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGAAAGCTCTCG‑ATCGAAGGGCAGGCGCACACCAGATTACGGTCGCCGAACACGATga > 7:133073/1‑148 (MQ=39) ataGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAc > 1:42620/1‑149 (MQ=255) taGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAcc > 5:385453/1‑149 (MQ=255) gCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGCATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAccc < 1:108875/149‑1 (MQ=255) gCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGCATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAccc < 1:103427/149‑1 (MQ=255) gCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg > 5:354401/1‑149 (MQ=255) ccaccaTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 3:93995/1‑149 (MQ=255) caccaTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGc > 1:375989/1‑149 (MQ=255) ccaTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCg < 4:93995/149‑1 (MQ=255) cATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggc < 2:42620/149‑1 (MQ=255) ttCGC‑GGG‑T‑AAACCCGCGCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggcgg < 8:396165/149‑1 (MQ=255) cgc‑gGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGCTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc < 3:46327/148‑1 (MQ=255) ggg‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAcc < 3:23599/130‑1 (MQ=255) ggg‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAcc > 4:23599/1‑130 (MQ=255) ggg‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg < 4:405423/132‑1 (MQ=255) ggg‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg > 3:405423/1‑132 (MQ=255) | TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCG‑ATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGC > NC_002947/1126498‑1126782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |