Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	6,013,885	T→C	intergenic (+90/+20)	xpt → / ← PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,013,885	0	T	C	100.0%	180.7 / NA	55	intergenic (+90/+20)	xpt/PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/30);  total (25/30)

GAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATC  >  NC_002947/6013739‑6014027                                                                                                                                                       |                                                                                                                                                gAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGc                                                                                                                                               >  5:149295/1‑149 (MQ=255)  aaTCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGGCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGc                                                                                                                                               <  3:46649/148‑1 (MQ=255)          gCCCGGGGGAAGTCGCTGGAAGGTTGTTTGGTCAGCTTCATCGAATAAACCCGAAACCTGCTTGGCGGG‑CAC‑‑‑GCCCGCTCCCACCGGTGCAGCACTGGGTTCGAACGCTGTGCCGTACCTGTGGGTGGGGGCGTGCCCGCGAATGGcc                                                                                                                                       <  8:362937/148‑1 (MQ=25)            ccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGCACGCGGTGCCGTACCGGTGGGAGCGGGCGTGCCCGCGCAATGCCGc                                                                                                                                     >  2:138652/1‑148 (MQ=38)                gTGAAGTCGCTGGAAGGTGGGGGGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGACCGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAAccc                                                                                                                                <  7:116891/149‑1 (MQ=255)                     gTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTa                                                                                                                           >  4:117307/1‑149 (MQ=255)                       cGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCtttt                                                                                                                            <  7:94124/146‑1 (MQ=255)                       cGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCCCAACCACACGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCATTGGGAGCCGGCGTGACCGCGAAAGGCCGCCACCCgttt                                                                                                                            >  8:94124/1‑146 (MQ=255)                               ggtggtGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCGTTTTATCTGGTTgcc                                                                                                                 <  2:90420/149‑1 (MQ=255)                                   gtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCa                                                                                                             <  3:117307/149‑1 (MQ=255)                                        gtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCCACCCAGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCa                                                                                                             <  1:138652/148‑1 (MQ=37)                                           aGCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGc                                                                                                      <  2:7692/148‑1 (MQ=255)                                            gCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCGCGAACGCTGTGCCGTACCAGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGcc                                                                                                     >  5:300881/1‑148 (MQ=255)                                                  tCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑CCCCGCTCCCACAGGTGCAGCACAGGTAGCGAACGCTGTGCCGTACCAGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCGTTTATCTGGTTGCCGCCAGCCCCGCcaacaac                                                                                               >  1:79784/1‑148 (MQ=38)                                                    gAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑CCCCCCAACCACCCGTGCAGCACAGGTCTAGAACGCTGTGCCGTACCTGTGGGAGCGGGGGTGCCCGCGAATGGCCGCCACCCTTTTATATGGTTGCCGCCCGCCCCCCCCACAACAAg                                                                                            >  8:224981/1‑149 (MQ=255)                                                         accccgACCCCTGCTGCGGGGG‑CAC‑‑‑GCCCGCTCCCACTGGGGCAGCACAGGTCTCGACCGCTGTGCCGTACCTGTGGTTGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                                       <  8:241716/145‑1 (MQ=255)                                                                  ccTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttc                                                                              <  7:115493/149‑1 (MQ=255)                                                                      cTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttctt‑c                                                                          <  6:149295/148‑1 (MQ=255)                                                                       ttCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttc                                                                              <  7:125011/144‑1 (MQ=255)                                                                       ttCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttc                                                                              >  8:125011/1‑144 (MQ=255)                                                                         ggcgGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑Cttt                                                                       <  2:147621/147‑1 (MQ=255)                                                                                      gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGCACCTGTGGGAACGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGc                                                             >  1:318399/1‑149 (MQ=255)                                                                                        ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCgg                                                           >  7:230052/1‑149 (MQ=255)                                                                                            tCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGcccc                                                                <  3:190905/140‑1 (MQ=255)                                                                                            tCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGcccc                                                                >  4:190905/1‑140 (MQ=255)                                                                                            tCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGc                                                       >  1:378073/1‑149 (MQ=255)                                                                                              ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGGTCCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCCACAACAAGCGCTGGTTAAATTCTT‑CTTTAAGCCCCTGCGGCTGccc                                                     >  2:115886/1‑149 (MQ=39)                                                                                               cacaGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGccc                                                     >  5:31448/1‑148 (MQ=21)                                                                                                 caGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCaa                                                  >  3:227180/1‑149 (MQ=255)                                                                                                   ggTGCAGCACAGGTCTCGAACGCTGGGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCAGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACt                                                <  6:300881/149‑1 (MQ=255)                                                                                                    gTGCAGCACAGGCCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCCTTTAAGCCCCTGCGGCTGCCCCAACt                                                <  1:215005/149‑1 (MQ=255)                                                                                                         gcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACt                                                >  7:362613/1‑143 (MQ=255)                                                                                                         gcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACt                                                <  8:362613/143‑1 (MQ=255)                                                                                                             aGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGccc                                      >  8:194795/1‑149 (MQ=255)                                                                                                                tctcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAg                                   >  6:429364/1‑149 (MQ=255)                                                                                                                  tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt                                 >  7:268620/1‑149 (MQ=255)                                                                                                                  tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCACCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGt                                 >  7:281631/1‑149 (MQ=255)                                                                                                                       cGCTGTCCCGTACGTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCtt                            <  1:150898/149‑1 (MQ=255)                                                                                                                        gCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCttt                           <  8:230052/149‑1 (MQ=255)                                                                                                                         cTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGccc                                                     <  4:237051/122‑1 (MQ=255)                                                                                                                         cTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGccc                                                     >  3:237051/1‑122 (MQ=255)                                                                                                                         cTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTc                          <  7:194795/149‑1 (MQ=255)                                                                                                                            tgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCAt                        <  8:268620/148‑1 (MQ=255)                                                                                                                            tgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATa                       <  3:164723/149‑1 (MQ=255)                                                                                                                              ccGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑Cttt                                                                       <  4:379220/99‑1 (MQ=255)                                                                                                                              ccGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑Cttt                                                                       >  3:379220/1‑99 (MQ=255)                                                                                                                              ccGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAgg                                  >  1:397488/1‑136 (MQ=255)                                                                                                                              ccGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAgg                                  <  2:397488/136‑1 (MQ=255)                                                                                                                                          gAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCg         >  5:18135/1‑149 (MQ=255)                                                                                                                                           aGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCgg        <  6:18135/149‑1 (MQ=255)                                                                                                                                            gCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGt       <  3:207279/149‑1 (MQ=255)                                                                                                                                               ggCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATa                       <  6:186106/130‑1 (MQ=255)                                                                                                                                                gCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTaa                                                                     >  3:29637/1‑83 (MQ=255)                                                                                                                                                gCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTaa                                                                     <  4:29637/83‑1 (MQ=255)                                                                                                                                                 cGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATc  <  4:227180/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                GAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGG‑CAC‑‑‑GCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTT‑CTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATC  >  NC_002947/6013739‑6014027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑