| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 97.4% | 105.6 / ‑6.8 | 39 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (14/24); total (14/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 97.4% | 109.1 / NA | 39 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (13/25); total (14/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCA‑CG‑‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCA > NC_002947/1499338‑1499613 || ccGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCt > 1:47151/1‑149 (MQ=255) ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg < 2:15566/149‑1 (MQ=255) gCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCgg > 8:9466/1‑149 (MQ=255) ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgt < 4:47155/149‑1 (MQ=255) ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgt < 6:212805/149‑1 (MQ=255) gCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtgt < 2:45015/149‑1 (MQ=255) cAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCAAAGCCGgtgcc < 8:232137/149‑3 (MQ=38) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgcgg < 8:203465/148‑6 (MQ=255) ccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGT‑TCGCGGGCACCC‑‑‑CC‑GCTcccc > 6:427297/1‑148 (MQ=25) aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑Ac < 4:113173/149‑1 (MQ=21) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGAtt < 6:307836/149‑1 (MQ=21) ttCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCa < 2:47151/149‑1 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAACGCGCCGCGGTGCAAGGCACCGGCCTTGCCCGTGT‑TCGCGGGCA‑CGC‑‑CC‑CCGCCCACCG‑ACGGATTGCCATTAAGCg > 3:322412/1‑149 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCGAAACACGCGCCCCCGTGCATGGCACCGGCGTTGCCGGTGT‑TAGCGGGCC‑CGC‑‑CC‑GCGCCCACAA‑ACTGATTGCCAATAACCg > 8:382161/1‑149 (MQ=255) aaCGAAGCCTTGGCACCACAGCAGGACTGCGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTGC‑GCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGcc < 7:382161/149‑1 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAGGCGCCCCCGTGCAAGGCACCCGCATTGCCGGTGT‑TAGCGGGCACCC‑‑‑CC‑CCACCCACAC‑ACAGAATTCCAATAATCggggcaa > 6:376745/1‑144 (MQ=16) aaGCCTTGGCAACACAGCAGGACTGAGGCTGCCAAACGGTGGGAGAGGGCCTAACCCCGAAACACGCGCCCCCGTGAAATGCAACAGCATTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTACCAAAA‑ACTGGTTGCCATTAATCGAGCCAATc > 6:112311/1‑149 (MQ=255) aGCCTGGGCACCACAGCAGGCCTGAGGCTGCCAACCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCg < 3:44334/149‑1 (MQ=18) gCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGc < 7:9466/149‑1 (MQ=21) cagcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg < 5:149742/81‑1 (MQ=255) cagcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg > 6:149742/1‑81 (MQ=255) cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGc < 2:66569/127‑1 (MQ=18) cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TAGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGc > 1:66569/1‑127 (MQ=18) aGGCTGCCAAACGGTGGGAGCGGGCGTGGCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg < 5:376745/148‑1 (MQ=21) cTGCCAAACGGTGGTTGCGGGCGTTCCCGCTAAACAGGCGCCGCTGTGCATGGCACGGGCTTTGCGGGTTT‑TCGCTGGAA‑CGC‑‑CC‑TCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc < 1:371091/149‑1 (MQ=255) gCCAAACGGTGGGTGGGGGCGTGCCCGCTAAACAGGCGCGGCGGTGCTTGGCACCGGCTTTGCCGGTGT‑TCGGGGGCA‑CGC‑‑CC‑GCTCCCACAGCACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 1:97892/143‑1 (MQ=21) ggggggCGCGGGCGTGCCCGCGAAACAGGGGCCGCGGTGCATGGGACCGGGTTTGCCGGTGT‑TGGCGGGCA‑CGCCCCC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttt < 1:323251/132‑1 (MQ=21) gggCGTGCCCGCTAAACTGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 3:349226/149‑1 (MQ=37) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 4:322412/149‑1 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 2:2673/138‑1 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc > 1:2673/1‑138 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt > 7:359184/1‑140 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 8:359184/140‑1 (MQ=25) ccGCTAAACTGGCGCCGCGGTGCATGGCCCCGGCTTTGCGGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:144332/149‑1 (MQ=37) aCAGGCGCCGCGGTGCATGGCACCGGCTGTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 5:427297/149‑1 (MQ=37) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa > 5:427143/1‑149 (MQ=37) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa > 1:347758/1‑149 (MQ=37) gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CG‑‑‑CCAGCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 6:392407/1‑148 (MQ=38) cgcgGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCA‑CGC‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCa < 6:427143/149‑1 (MQ=37) || CCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCA‑CG‑‑‑CC‑GCTCCCACAG‑ACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCA > NC_002947/1499338‑1499613 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |