Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,932,640	+C	intergenic (‑107/+43)	rluA ← / ← minE	bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,932,638	1	.	C	94.5%	179.3 / 3.4	55	intergenic (‑105/+45)	rluA/minE	bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base C (27/25);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑G‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACAT  >  NC_002947/1932500‑1932777                                                                                                                                              |                                                                                                                                             cTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCTACTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑G‑CCAGCGAAgg                                                                                                                                    >  1:233483/1‑149 (MQ=255)  ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGGGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAggg                                                                                                                                   >  6:19111/1‑149 (MQ=255)  ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCACCTACAAAGGAGATGCGTCCCCGGGAGGGGCGGGC‑TTGG‑CC‑GCGAAggg                                                                                                                                   >  6:12932/1‑149 (MQ=38)     gAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTg                                                                                                                                >  4:309926/1‑149 (MQ=255)           gATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGATATTCGTCCCCGGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTggcagg                                                                                                                           >  8:358978/1‑143 (MQ=38)            aTCTGTACATTCGACAGCGGCTTTGCAGGTCTCACTAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGc                                                                                                                          <  8:214133/148‑1 (MQ=38)             tCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGccg                                                                                                                        >  4:308926/1‑147 (MQ=255)                 gACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGccc                                                                                                                     >  5:87263/1‑148 (MQ=255)                 caCATGCGCCAGCGGCATGGCAGGTCGCACCAAAATAGCAGTAACCGCTGGGGCCGCTTGGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGCCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGcc                                                                                                                      <  3:111444/146‑1 (MQ=37)                    aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCTCCTACAAAAGATATTCGTCCCCGGTATGAGCGGGCGTG‑G‑CC‑GCGAAGGGCAGCAAGGCCGCCCCaaa                                                                                                                 >  2:227193/1‑149 (MQ=37)                             cGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAc                                                                                                         >  4:348489/1‑148 (MQ=255)                              ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCGCCGACAAAGGTTATGCCTGCCCGGTAGGAGCGGGC‑TT‑GCCC‑CCGAAGGGCATCAAGGCAGCCCCAAATTTTCgcca                                                                                                        >  5:254608/1‑145 (MQ=37)                                        gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATTTGCGTCCCCGGGAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGCCTACGCGGCTCAg                                                                                             >  7:21461/1‑149 (MQ=37)                                            cACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAt                                                                                         <  3:348489/149‑1 (MQ=255)                                             aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc                                                                                        >  8:412335/1‑149 (MQ=255)                                             aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc                                                                                        <  3:309926/149‑1 (MQ=255)                                              ctaaaaTAGCAGTAAACGGTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAACGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATcc                                                                                       <  1:227193/147‑1 (MQ=255)                                                       cacaaaccGCTGGGCCCGCTTCGCGGCCCGTCGCGGGCAAGCCCGCTCCAAAAAACCATATGCGCCCCCTGTAGGAGCGGGC‑TG‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTg                                                                              <  7:358978/142‑1 (MQ=37)                                                       cAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTg                                                                              <  3:119264/149‑1 (MQ=255)                                                            aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGTCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAggg                                                                                                                                   >  5:219076/1‑91 (MQ=255)                                                            aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGTCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAggg                                                                                                                                   <  6:219076/91‑1 (MQ=255)                                                            aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGa                                                                                                          >  4:326423/1‑116 (MQ=255)                                                            aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGa                                                                                                          <  3:326423/116‑1 (MQ=255)                                                             ccgcgGGGGCCGCTTTGCGGCCCTGCGCGGGCACGCCCGCTCCTACAACGGATATGCGCCCCCTTTAGGTGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc                                                                        <  5:23831/148‑1 (MQ=255)                                                               gCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg                                                                      <  6:216383/149‑1 (MQ=255)                                                                       cGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGaa                                                              <  3:392939/149‑1 (MQ=255)                                                                        gCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAAt                                                             >  3:75915/1‑149 (MQ=255)                                                                         cTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAAAGCATAGGCGGCCCCTGTAGGAGGGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc                                                            <  3:198037/149‑1 (MQ=38)                                                                                  ccTTCGCGGGCAAGCCCGCTCCTACAAAGGATAGGCGCCCCCTGTCGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCt                                                                    <  7:183/132‑1 (MQ=38)                                                                                            cacccccGCTCCAACAAAGGATATCCGCCCCCTGTAGCCGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt                                         <  8:187146/145‑1 (MQ=255)                                                                                               gCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc                                        >  4:407579/1‑147 (MQ=255)                                                                                                cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGc                                                                     <  4:75832/117‑1 (MQ=255)                                                                                                cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGc                                                                     >  3:75832/1‑117 (MQ=255)                                                                                                  cGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc                                        >  5:215576/1‑144 (MQ=255)                                                                                                  cGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc                                        <  6:215576/144‑1 (MQ=255)                                                                                                   gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc                                  >  2:25904/1‑149 (MQ=255)                                                                                                        tACAAAGGATATGCGTCCCCTGTAGGAGCGNGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat                             >  1:37343/1‑149 (MQ=255)                                                                                                        tACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTc                                                                                                            >  6:335496/1‑70 (MQ=255)                                                                                                        tACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat                             >  1:30801/1‑149 (MQ=255)                                                                                                           aaaGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca                          >  3:158189/1‑149 (MQ=255)                                                                                                            aaGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt                                               <  3:348090/127‑1 (MQ=255)                                                                                                            aaGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt                                               >  4:348090/1‑127 (MQ=255)                                                                                                            aaGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc                                       <  6:113270/135‑1 (MQ=255)                                                                                                            aaGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc                                       >  5:113270/1‑135 (MQ=255)                                                                                                                      gTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg               >  8:289759/1‑149 (MQ=255)                                                                                                                       tCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATTATCGTTGc              <  2:37343/149‑1 (MQ=255)                                                                                                                       tCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATTATCGTTGc              <  2:30801/149‑1 (MQ=255)                                                                                                                             gTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGt        >  6:212111/1‑149 (MQ=255)                                                                                                                              tAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt       >  8:95723/1‑149 (MQ=255)                                                                                                                                ggAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca     <  7:412335/149‑1 (MQ=255)                                                                                                                                ggAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc      <  3:407579/148‑1 (MQ=255)                                                                                                                                 gAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca     >  4:371589/1‑148 (MQ=255)                                                                                                                                 gAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca     >  2:215169/1‑148 (MQ=255)                                                                                                                                   gCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACat  <  5:19111/149‑1 (MQ=255)                                                                                                                                   gCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACat  <  5:12932/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                             CTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑G‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACAT  >  NC_002947/1932500‑1932777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑