Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	336,125	+T	coding (210/237 nt)	PP_0278 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	336,124	1	.	T	100.0%	193.4 / NA	56	D70V (GAT→GTT)	PP_0278	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (28/28);  total (28/28)

GTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCA  >  NC_002947/335976‑336261                                                                                                                                                         |                                                                                                                                           gTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGa                                                                                                                                             <  6:7109/149‑1 (MQ=255)     aCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGGGTCCCCGGCGACCCTCCTCTCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑t                                                                                                                                           <  1:353437/149‑1 (MQ=255)       ccATCCCTCAATCTCCTACGCGTAGAGCCCGGAGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCTAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacct                                                                                                                                      >  2:125257/1‑144 (MQ=255)              tCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGa                                                                                                                                 >  3:276016/1‑148 (MQ=255)               cAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACa                                                                                                                               >  6:66485/1‑149 (MQ=255)                     ccTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGcc                                                                                                                          >  4:344581/1‑148 (MQ=255)                       tACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAg                                                                                                                       >  2:174227/1‑149 (MQ=255)                           cgTAGAGCCCGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGgcgc                                                                                                                   <  8:317886/149‑1 (MQ=255)                             tAGAGCCCGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCt                                                                                                                  >  5:216943/1‑148 (MQ=255)                               gagCCCGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtgat                                                                                                               >  8:318990/1‑149 (MQ=255)                               gagCCCGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtga                                                                                                                <  2:268037/148‑1 (MQ=255)                                agCCCGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGTCGCtgat                                                                                                               >  8:179342/1‑148 (MQ=255)                                  cccGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGaa                                                                                                            >  6:100153/1‑149 (MQ=255)                                   ccGGCGGCCCCGTCGACTGGTC‑‑‑CTACGAGCATGTCTCTATCCTGATGTGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCAGATTACCTGTGCGACATTGCCAAGGCGCTGATGAAc                                                                                                           <  8:363887/149‑1 (MQ=255)                                      gCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGat                                                                                                        <  1:174227/149‑1 (MQ=255)                                           cccGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                     >  3:259795/1‑147 (MQ=255)                                            ccGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGTACGATATc                                                                                                     >  6:408122/1‑146 (MQ=255)                                                    gcgGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCAGGTGCGCGAGGGCGATATCGAGGACGACCATAAATTCCTGGCGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTCATGAACGAAATCGAGATCGcc                                                                                            >  3:375047/1‑147 (MQ=255)                                                      ggC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAgg                                                                                        <  4:276016/149‑1 (MQ=255)                                                       gC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAggg                                                                                       >  6:218452/1‑149 (MQ=255)                                                            cTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGc                                                                                                                                   >  2:394158/1‑103 (MQ=255)                                                               cGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtgat                                                                                                               >  4:285719/1‑120 (MQ=255)                                                               cGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtgat                                                                                                               <  3:285719/120‑1 (MQ=255)                                                                    aTGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAt                                                                             >  1:380971/1‑149 (MQ=255)                                                                     tGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAtt                                                                            <  2:380971/149‑1 (MQ=255)                                                                      gTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAg                                                                                                                       >  6:304573/1‑105 (MQ=255)                                                                      gTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAg                                                                                                                       <  5:304573/105‑1 (MQ=255)                                                                       tctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAg                                                                          <  4:259795/149‑1 (MQ=255)                                                                       tctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAg                                                                          >  2:89455/1‑149 (MQ=255)                                                                         tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACAt                                                                                                                              >  8:57938/1‑95 (MQ=255)                                                                         tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACAt                                                                                                                              <  7:57938/95‑1 (MQ=255)                                                                         tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAgg                                                                         >  4:238403/1‑148 (MQ=255)                                                                         tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAgg                                                                         >  6:213980/1‑148 (MQ=255)                                                                            aTCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGTACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAtt                                                                     <  5:408122/149‑1 (MQ=255)                                                                             tCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTg                                                                    <  3:241197/149‑1 (MQ=255)                                                                               cTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGAGATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAgg                                                                                  >  7:230238/1‑133 (MQ=255)                                                                               cTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAgg                                                                                  <  8:230238/133‑1 (MQ=255)                                                                                tGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcg                                                                 >  4:13965/1‑149 (MQ=255)                                                                                tGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcg                                                                 >  1:89804/1‑149 (MQ=255)                                                                                   tGGGCTACATCAAGCACCTGGTGCGCGATGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcgcg                                                               <  3:344581/148‑1 (MQ=255)                                                                                          cATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtg                                                       <  3:289869/149‑1 (MQ=255)                                                                                          cATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtg                                                       <  2:342764/149‑1 (MQ=255)                                                                                           aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                      <  5:218452/149‑1 (MQ=255)                                                                                            tCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                     <  3:238403/149‑1 (MQ=255)                                                                                             cAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCtgtg                                                                                                                                    <  6:261573/69‑1 (MQ=255)                                                                                              aaGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                    <  5:213980/148‑1 (MQ=255)                                                                                              aaGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGAACGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                    <  1:89455/148‑1 (MQ=255)                                                                                                 cACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCATCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGc                                                <  4:298181/149‑1 (MQ=255)                                                                                                          cgcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGc                                       <  2:89804/148‑1 (MQ=255)                                                                                                            cgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGACGAACGATATCGAGTTCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTc                                    <  1:125257/149‑1 (MQ=255)                                                                                                               gggCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGAAAGGCGCATAAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑ATGccgccgg                                 >  3:296849/1‑143 (MQ=255)                                                                                                                         gaggaCGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCTGc                       <  5:66485/149‑1 (MQ=255)                                                                                                                            gacgacCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCTGCa                      >  5:123987/1‑147 (MQ=255)                                                                                                                               gacCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCTGCAAagca                 <  6:123987/149‑1 (MQ=255)                                                                                                                                 ccATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCTGCAAagcagc               >  6:362612/1‑149 (MQ=255)                                                                                                                                      aaTTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCGTGGGGGGGCGATTGGGTTGCAAGCCGGCCCCg           >  1:189978/1‑149 (MQ=255)                                                                                                                                              ggggCAGGCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTTAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCa  <  4:375047/149‑1 (MQ=255)                                                                                                                                                   aGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                     <  4:68358/94‑1 (MQ=255)                                                                                                                                                   aGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                     >  3:68358/1‑94 (MQ=255)                                                                                                                                                         |                                                                                                                                           GTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGC‑‑‑CTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCA  >  NC_002947/335976‑336261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑