| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,126,647 | +C | intergenic (‑101/+48) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 79.5% | 101.2 / 23.6 | 39 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (8/0); new base C (12/19); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.28e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126503‑1126787 | gggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGCCATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTAAACGCGCATAAGCGGGGATAGCCACCATAG‑CAATAGCCGG‑T‑taatcccca > 3:394995/1‑143 (MQ=255) atgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTTACCGCGAATGCGGAGGTATATCCACCAAAG‑CATTAGCGGG‑T‑AAACCCGCTCCTaa > 3:68676/1‑147 (MQ=38) gAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTTAACCCGAATTGGGAGGTTTATGCACCATCG‑CATTAGCGGGTT‑AAC‑CCGCGCCAACACAGAGCCCGcc > 7:356358/1‑149 (MQ=255) gAACAGGGCCAAGACGCGCAGGGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACaga < 8:395544/142‑1 (MQ=37) aaGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTAACCGCGAATGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑TAAAA‑CCGCGACTACACAGAGCCCGCCCCCCAAGc > 2:275675/1‑147 (MQ=39) aCATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAg < 2:143082/148‑1 (MQ=255) aatgggtaCCTCCTGCCTGGTTTTTGGGTGTACATTGAGCCCTTTTGGGGG‑‑GGGTTTACCCGCTAATGCGTTGTTATAGCCACCATCG‑CATTCGGGGT‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAggg < 8:356358/142‑1 (MQ=255) gtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTTACCCCCAAAGCGGAGGTTAAGCCAACAACG‑CAATAGCGGG‑TAAAA‑CCGCGCCCAAACACAGCCCGCCCCCCCACCCACGTGGGGGGGGGGGCTa > 8:45016/1‑149 (MQ=255) gtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTAACCCCGCATGGGGAGGTTTTTGCACCCTAG‑CCTTAGCGGG‑TGAAC‑CCCCGCCGACAAACAGCCCCCCCCCCCACCCCCGGGGCGGGGGGGGcga > 8:246049/1‑147 (MQ=255) gACTTCTTGCCAGGTTGTTGTGTGGACATTGAGCCCTGTAGGCGC‑‑TGGTTTACCCGCTATTGCGGAGGTATAGCCACCATCG‑CATTCTCGGT‑T‑AAACCCGCTACTACACAGAGCACGCCACCCAAGCCAg < 2:269580/130‑1 (MQ=255) gACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑TAAAA‑CCGCTCCTACACAGAGCCCGCCCCCCAAGCCAg > 1:269580/1‑130 (MQ=255) ctGCCAGTTTGTTTGGTGTAAATTGAGCCCTGTAGCCGC‑‑GGGTTATCGGTCTAATGCGTAGGTATAGCTACATTCG‑CATTCGGGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTc < 7:270034/149‑1 (MQ=255) ctGCCAGGTTTTTTGGTGGACATTGAGCCCTGTAGGCGC‑‑GGTTTTACCCGCGAATGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTc < 7:270040/149‑1 (MQ=38) ctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGGGTTTACCCCCCAATGCGGGGGTATAGCCACCAACG‑AAATCGCGGG‑TAAAA‑CCCCCCCTACAAACACCCCCCCCCCCCACCCCGGGGGGGGGGGGGGCTATAAcgcg > 2:92236/1‑149 (MQ=255) ctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTAACCCCGAATGCGGAGGTATATCCACCAACG‑CATTAGCGGGTT‑AAA‑CCGCACCAAACAAGATCCCGCCCCCCAACCCAGCTGGAGGGGAGGGCTATAAGGCGTc > 7:386495/1‑149 (MQ=255) ccATGTTTTTGGGTGTAAATTGAGCCCTGTAGGCGC‑‑GGGTTTACCCGCGAATGCGGTGGTATAGCCACCATCG‑AATTCGCGGT‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTg < 4:68676/149‑1 (MQ=37) gttgttGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGACGCCTGATAg < 6:325501/149‑1 (MQ=38) gttgttGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTAACCCCGAAATCGGCGGGATAGCCACCAAAGCCATAGCCGGG‑A‑AAA‑CCCCGCCGACAAAAAGCCCCCCCCCCCACCCACGTGGGGGGGGGGGCTATAAcgcgggcgga > 4:380938/1‑140 (MQ=255) tGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTTACCCCGAATGGGGAGGGATAGGCACCATCG‑CATTAGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCCCCCCAGCCAGGGGGGGGGGCGGGCGATCAGGCGGCCGGATAGcgagcgat > 8:192227/1‑141 (MQ=255) tGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcgat < 7:192227/148‑1 (MQ=255) ttGAGCCCTGTAGGCGC‑‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACggg < 3:380938/149‑1 (MQ=255) gcgc‑‑GGGTTTACCCGCGAATGGGGAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGcaccc > 6:217120/1‑145 (MQ=255) gcgc‑‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCNCCCAAGCCAGGTGGCGGGGAGGGCTATCAg < 1:40067/109‑1 (MQ=255) gcgc‑‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCG‑CATTAGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg > 2:40067/1‑109 (MQ=255) cgc‑‑gGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTACTACACAGAGCCCGCCCCCCAAGCCCGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGCCGGGCAGGCCCCCACCAg > 4:373715/1‑149 (MQ=38) aTGCGGAGGTATCGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAac < 7:359107/148‑1 (MQ=255) tGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAacac < 4:394995/149‑1 (MQ=255) ggAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt > 3:375951/1‑149 (MQ=255) ggAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt > 1:2628/1‑149 (MQ=255) taGCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCTCACCAGGTTGCGGTCGCCGAACACGTTGTCGAcc < 1:275675/149‑1 (MQ=255) gCCACCATCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg > 1:295931/1‑149 (MQ=255) ccaccaTCG‑CATTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg < 1:92236/149‑1 (MQ=255) ttttCGAGGT‑T‑AAACCCGCTCCTACACAGAGCCCGCCACACAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGTTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggc < 8:386495/147‑1 (MQ=255) aTTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggcg > 1:189285/1‑149 (MQ=255) aTTCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGGGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggcg < 7:200392/149‑1 (MQ=255) ttCGCGGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggcgg < 1:27291/149‑1 (MQ=255) gcgGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc > 6:382468/1‑147 (MQ=255) cgGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAg < 5:382468/149‑1 (MQ=255) gg‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTa > 5:357621/1‑149 (MQ=255) gg‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTa > 4:18196/1‑149 (MQ=255) | GGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCG‑CATTCGCGGG‑T‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126503‑1126787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |