Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,537,0470CA22.9% 95.6 / 12.7 49intergenic (+24/+79)nadE/PP_4870NH(3)‑dependent NAD(+) synthetase/azurin
Reads supporting (aligned to +/- strand):  ref base C (13/24);  new base A (10/1);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.52e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGTGACCTACGCGCAGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTC  >  NC_002947/5536899‑5537194
                                                                                                                                                    |                                                                                                                                                    
gCGTGACCTACGCGCAGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTc                                                                                                                                                      <  7:223418/149‑1 (MQ=255)
   tGACCTACGCGCAGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCAcc                                                                                                                                                   <  4:22137/149‑1 (MQ=255)
       cTACGCGCAGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCcgc                                                                                                                                                >  5:182306/1‑148 (MQ=255)
            cgcAGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATAc                                                                                                                                          <  2:431150/149‑1 (MQ=255)
               aGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAg                                                                                                                                       <  1:366894/149‑1 (MQ=255)
                gATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAgg                                                                                                                                      <  3:403401/149‑1 (MQ=255)
                gATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACAAgg                                                                                                                                      <  4:186920/149‑1 (MQ=255)
                   cGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAAACCGCGCATACGAGGGTg                                                                                                                                   >  5:86593/1‑149 (MQ=255)
                    gATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTg                                                                                                                                   <  8:19474/148‑1 (MQ=255)
                          ttCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCAc                                                                                                                            >  5:75624/1‑149 (MQ=255)
                              tGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGTCCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAAc                                                                                                                                >  5:359725/1‑141 (MQ=255)
                              tGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGTCCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAAc                                                                                                                                <  6:359725/141‑1 (MQ=255)
                                cATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGTTTCACCCGCGAATACGAGGGTGAACCCACCGACg                                                                                                                       <  3:126474/148‑1 (MQ=255)
                                cATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACg                                                                                                                       >  2:89262/1‑148 (MQ=255)
                                    ggCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGCATAAGCGGGTGAACCCACCGCCCCAt                                                                                                                    >  8:390073/1‑147 (MQ=255)
                                        gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                              >  3:49081/1‑149 (MQ=25)
                                            gTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTg                                                                                                           >  6:101438/1‑148 (MQ=255)
                                                     gCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCGGGTGAACCCGCTcc                                                                                                 <  4:49081/149‑1 (MQ=21)
                                                      cAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCCAAAAAGAAGGTGAACCCACCGACCCAATAGCGGGTGAACCCCCta                                                                                                  >  6:157233/1‑146 (MQ=255)
                                                       aGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGAGGGTTAACCCGCGAATAACACGGTGAAACCACCGACGCCATCCCGGGTGAACCCGCGccag                                                                                               >  8:230781/1‑147 (MQ=255)
                                                         cGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCCCGAAAACGAGGGTGAACCCACCCACGCATTAGCGGGTGAACCCGCGCCTg                                                                                               >  3:102541/1‑147 (MQ=255)
                                                              gACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCggga                                                                                                            <  7:341778/129‑2 (MQ=255)
                                                              gACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCggga                                                                                                            >  8:341778/1‑128 (MQ=255)
                                                               aCATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGGTCACCCGCGAATAAGAGGGTGAACCCACCGCCGCCTTCGCGGGTGAAACCGCACCTGCAG‑GGTTc                                                                                      >  6:81671/1‑149 (MQ=255)
                                                                        tcgCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGAc                                                                                                                        <  4:198958/107‑1 (MQ=255)
                                                                        tcgCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGAc                                                                                                                        >  3:198958/1‑107 (MQ=255)
                                                                           ccACCTACCGCAAGACCCAGCCCAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCtt                                                                          <  7:390073/149‑1 (MQ=255)
                                                                                  ccGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTAc                                                                   <  6:75624/149‑1 (MQ=255)
                                                                                   ggCAACACCCAGCACACGGGCTATCTGCCGTTCGGTCCTTTATATGTGGCGTCCCGGTGGGGTTTAACCGGCTATTACGCGGGTTATCCCACCGACGCATTCGGGGGTTAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACt                                                                  <  4:102541/148‑1 (MQ=255)
                                                                                      aaGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGa                                                               <  5:81671/149‑1 (MQ=255)
                                                                                           ccAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCCCGAAGAAGAGGGTGAACCCACCGCCCCATTAGCGGGTGAACACGCGCCAGCAC‑GGTTAGCCGTCAGCCTTTGATTACTTGaacat                                                           >  6:384618/1‑144 (MQ=255)
                                                                                               cacaAGCGCGAACTGCCGTTCGCTCCTTTATAAGTGGCCTGCAGCAGCGGGTTCACCCGCGAATACTAGGGTGAACCCACCGACGCATTCGCGGGTTAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCt                                                      <  5:157233/149‑1 (MQ=255)
                                                                                                acaAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTTACCCCCGGATAAGCGGGTGGACCCCCCCACCCCATAGCGGGGGGACCCCCGCCAGGAG‑GGGTGGCCGGGAGGCGTTGGATTCTTTACGCTGAccag                                                     >  4:407555/1‑147 (MQ=255)
                                                                                                 caAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGAATACGAGGGTGAACCCACCCACCCATTAGCGGGTGAACCCGCTCCAGCAG‑GGTTAGCCGTCAGCCGTTGATTAAATGACGATGACCTTg                                                    >  6:191251/1‑149 (MQ=255)
                                                                                                 caAGCGCGAACTGCCGTCGGCTCCTTGATAAGTGGCCTGCAGGTGCGGGTTCACCCGCTAATACGAGGGTTAACCAACCTACTCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCTTCAGCCTTTGATTACTTGACGATGACCTTg                                                    <  7:230781/149‑1 (MQ=255)
                                                                                                 aaggagcgAACGGCAGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTg                                                    >  1:318279/6‑149 (MQ=255)
                                                                                                        gAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGAGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTtc                                              <  1:89262/149‑1 (MQ=255)
                                                                                                              ccGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCatcatc                                        <  5:191251/148‑1 (MQ=255)
                                                                                                                   cGCTCCTTGATAAGTGGCCTGCAGGAGGGGGTTCACCCGCGAATACGAGGGTTAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGAt                                   <  6:128637/148‑1 (MQ=255)
                                                                                                                      tCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCTAATACGTGGGGGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTgg                               <  6:182306/149‑1 (MQ=255)
                                                                                                                      tCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTtcatc                                           <  6:207633/137‑1 (MQ=255)
                                                                                                                      tCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTtcatc                                           >  5:207633/1‑137 (MQ=255)
                                                                                                                      tCCTTGATAAGTGGCCTGCAGGAGCGGGTTAAACCGCGAATAAGATGGTGAACCCACCCACGCATTAGCGGGTGAAACCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTAAATTACCATGAACTTGCCTTTCATAATACAGATGTgg                               >  2:70506/1‑149 (MQ=255)
                                                                                                                           gATAAGTGGCCTGCAGGAGCGGGTTAACCCCCCCATAAGCAGGTGAAACCACCCCCCCAATCACGGGTTAAACCCCTCCAGAAA‑GGTGCGCCGACATCCAGTGATTAATTTACCCTTACCATGCCGTATATCATCGACAATTGGCCAgg                          >  6:431768/1‑149 (MQ=255)
                                                                                                                               aGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAg                      <  5:101438/149‑1 (MQ=255)
                                                                                                                                   gCCTGCAGGCGCGGGTTCACCGGCTAATACGAGGGTCAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGt                    <  7:47831/147‑1 (MQ=255)
                                                                                                                                      atctggAGCGGGTTCACCCGCTAATACGAGGGTTAACCCACCGACGCTTTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCaga               <  1:235963/145‑1 (MQ=255)
                                                                                                                                           gAGCGGGTTAACCCGCGAAAACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTAGCCGTCAGCCTTTGATTAATTGAAGATGACCTTTCCTTTCATAATAGAGATGTGGCCCGGGAAGGTTCagaaata           >  2:235963/1‑145 (MQ=37)
                                                                                                                                                gttccACCCGCGAATCCGAGGGTGAACCCACCGTCGCATGCGGGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTa     <  7:158891/145‑1 (MQ=255)
                                                                                                                                                 gTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAg    >  1:228656/1‑149 (MQ=255)
                                                                                                                                                  ttCACCCTCGTATTCGCGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGt   >  7:73929/1‑149 (MQ=255)
                                                                                                                                                    cACCCGCTAATAGGTGGGGAAACCCACGGACGCTTTCGCGGTTTAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTc  <  5:431768/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGTGACCTACGCGCAGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAG‑GGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTC  >  NC_002947/5536899‑5537194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑