Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 2,051,204 T→G T159P (ACC→CCC)  PP_1826 ← isochorismatase family hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,051,2040TG100.0% 165.6 / NA 48T159P (ACC→CCC) PP_1826isochorismatase family hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/23);  total (25/23)

GCCCGGCTGCCGGGCGGTCCTGCAAGCCGCTCAGATCAGGCTGGCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGTGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGTGGCCCAGCTCCTGCAATGTTTCGTGCAGCTTGG  >  NC_002947/2051058‑2051347
                                                                                                                                                  |                                                                                                                                                
gCCCGGCTGCCGGGCGGTCCTGCAAGCCGCTCAGATCAGGCTGGCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACgggggg                                                                                                                                                <  7:103127/149‑1 (MQ=255)
 cccGGCTGCCGGGCGGTCCTGCAAGCCGCTCAGATCAGGCTGGCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGACATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGc                                                                                                                                               <  6:25248/149‑1 (MQ=255)
    ggCTGCCGGGCGGTCCTGCAAGCCGCTCAGATCAGGCTGGCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGa                                                                                                                                            >  8:121930/1‑149 (MQ=255)
                  ccTGCAAGCCGCTCAGATCAGGCTGGCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTAc                                                                                                                                >  3:94911/1‑147 (MQ=255)
                   cTGCAAGCCGCTCAGATCAGGCTGGCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAg                                                                                                                             <  7:121930/149‑1 (MQ=255)
                     gCAAGCCGCTCAGATCAGGCTGGCGGTGGGGGCCACGAAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAggg                                                                                                                           >  7:244990/1‑149 (MQ=255)
                      cAAGCCGCTCAGATCACGCTGGCGGGGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAgg                                                                                                                            <  2:351691/147‑1 (MQ=255)
                          ccGCTCAGATCAGGCTGGCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCAc                                                                                                                      <  5:394680/149‑1 (MQ=255)
                                 gATCAGGCTGGCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAc                                                                                                               >  2:316007/1‑149 (MQ=255)
                                     aGGCTGGCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATa                                                                                                          <  1:231097/149‑1 (MQ=255)
                                     aGGCTGGCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATa                                                                                                          <  2:190133/149‑1 (MQ=255)
                                      ggCTGGCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAg                                                                                                         <  5:314975/149‑1 (MQ=255)
                                           gCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCt                                                                                                     <  3:268621/148‑1 (MQ=255)
                                           gCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACGTGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCt                                                                                                     <  7:173832/148‑1 (MQ=255)
                                            cGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTg                                                                                                   <  1:316007/149‑1 (MQ=255)
                                                  gggCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAggg                                                                                                                           <  6:329178/120‑1 (MQ=255)
                                                  gggCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAggg                                                                                                                           >  5:329178/1‑120 (MQ=255)
                                                      cACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAc‑c                                                                                                             >  2:286416/1‑129 (MQ=255)
                                                      cACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAc‑c                                                                                                             <  1:286416/129‑1 (MQ=255)
                                                           aaGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACggtg                                                                                    >  5:261222/1‑149 (MQ=255)
                                                           aaGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACggtg                                                                                    <  4:94911/149‑1 (MQ=255)
                                                                       cAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTg                                                                        <  8:244990/149‑1 (MQ=255)
                                                                         gCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTgg                                                                       >  4:189501/1‑148 (MQ=255)
                                                                          ccATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGaa                                                                     >  6:168624/1‑149 (MQ=255)
                                                                            aTCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGCCGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGCCGCGTCTTCTACCAGGGTGCACCGATAAC‑GATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATg                                                                   >  6:84954/1‑149 (MQ=255)
                                                                                 ggCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCa                                                              >  5:158974/1‑149 (MQ=255)
                                                                                 ggCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCa                                                              >  2:39664/1‑149 (MQ=255)
                                                                                     aTTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGa                                                           >  2:41552/1‑148 (MQ=255)
                                                                                       ttCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAAc                                                        >  5:124836/1‑149 (MQ=255)
                                                                                         cGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGCCGCGGCGTCTCCCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACca                                                      >  7:402842/1‑149 (MQ=255)
                                                                                          gCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACca                                                      >  2:181866/1‑148 (MQ=255)
                                                                                           cccTGGGGACTCTGCGCGGCCGGCATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATACC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACcaca                                                    <  8:402842/147‑1 (MQ=255)
                                                                                            aCTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACca                                                      >  7:36737/1‑146 (MQ=255)
                                                                                             cTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGa                                                  <  5:168624/149‑1 (MQ=255)
                                                                                                            ggCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGt                                   >  4:184499/1‑149 (MQ=255)
                                                                                                                      aCGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGTGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGTGGCCCAGCt                          <  6:261222/148‑1 (MQ=255)
                                                                                                                      aCGCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGTGGCCCAGCTc                         >  8:190979/1‑149 (MQ=255)
                                                                                                                        gCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACggt                                                                                     <  3:308495/87‑1 (MQ=255)
                                                                                                                        gCCGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACggt                                                                                     >  4:308495/1‑87 (MQ=255)
                                                                                                                          cGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGTGGCCCAGCTCCTg                      >  7:61597/1‑148 (MQ=255)
                                                                                                                          cGTCCTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGTGGCCCAGCTCCTGc                     <  6:124836/149‑1 (MQ=255)
                                                                                                                              cTTGAATGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGTGGCCCAGCTCCTGCAAt                  <  6:158974/148‑1 (MQ=255)
                                                                                                                                   aTGCCTGGTCAGGGGGGGCCGACGCGTGTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCTAACGGTGGTGCTGACGCTGGAATGGCTCATGATACCACAGACGATCAGGTGCAGGTGGCCCAGCTc                         <  2:321884/136‑1 (MQ=255)
                                                                                                                                   aTGCCAGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGGGCTGACGCGGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGTGGCCCAGCTCCTGCAATGTTTCg            <  2:261195/149‑1 (MQ=255)
                                                                                                                                   aTGCCAGGTCACGGGGGGCCGACCCGTCTTCTACCAGGGTGCACCGATAACGGATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCACGTGGCCCACCTc                         >  1:321884/1‑137 (MQ=255)
                                                                                                                                        aGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGTGGCCCAGCTCCTGCAATGTTTCGTGCAg       <  3:189501/149‑1 (MQ=255)
                                                                                                                                        aGGTCACGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGTGGCCCAGCTCCTGCAATGTTTCGTGCAg       >  5:281044/1‑149 (MQ=255)
                                                                                                                                             aCGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGTGGCCCAGCTCCTGCAATGTTTCGTGCAGCTTgg  >  5:56543/1‑149 (MQ=255)
                                                                                                                                             aCGGGGGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGTGGCCCAGCTCCTGCAATGTTTCGTGCAGCTTgg  >  8:410682/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCCCGGCTGCCGGGCGGTCCTGCAAGCCGCTCAGATCAGGCTGGCGGTGGGGGCCACGCAAGCGAAGTTGTCAGCCATCACGGCCATTTCGCACTGGTGAATCTGCGCGGCCGGAATGACGCCGTCCTTGAATGCCAGGTCACGGGTGGCCGACGCGTCTTCTACCAGGGTGCACCGATAAC‑CATAGTCCTTGGCGCGGCGAACGGTGGTGCTGACGCTGGAATGGCTCATGAAACCACAGACGATCAGGTCCAGGTGGCCCAGCTCCTGCAATGTTTCGTGCAGCTTGG  >  NC_002947/2051058‑2051347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑