Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,278	C→T	intergenic (+515/+238)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,278	0	C	T	100.0%	132.7 / NA	39	intergenic (+515/+238)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/18);  total (21/18)

TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCT‑CC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTC  >  NC_002947/4741135‑4741413                                                                                                                                                 |                                                                                                                                          tGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGtcct‑cc                                                                                                                                     >  5:207532/1‑149 (MQ=255)    tCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAAATGGGTATAGGTCCAGCTCTGGTCACCTTCGTGATTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCAAGGCACAGGACCCGGACACGtcct‑cctg                                                                                                                                   >  7:274226/1‑146 (MQ=255)            tgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGcgcctc                                                                                                                          <  4:321193/149‑3 (MQ=255)              cagcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGcgcctcga                                                                                                                        <  3:308558/149‑5 (MQ=255)               agcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGcgcctcgac                                                                                                                       >  5:355393/1‑144 (MQ=255)                 caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGcgcctcgacct                                                                                                                     >  3:127353/1‑142 (MQ=255)                 caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGcgcctcgacct                                                                                                                     <  2:84971/149‑8 (MQ=255)                           cgcgGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTACAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAgcgg                                                                                                           >  3:37814/1‑149 (MQ=255)                            gcggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAgcggc                                                                                                          >  1:101076/1‑149 (MQ=255)                                  tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                                                     >  4:44637/1‑148 (MQ=255)                                  tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                                                     >  3:182147/1‑148 (MQ=255)                                  tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                                                     >  3:188835/1‑148 (MQ=255)                                       ggCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc                                                                                               <  8:27702/149‑1 (MQ=255)                                        tccATTGCTTATTGAACTGTGTATATGTCCAGCTCTGTTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                                              <  8:274226/148‑1 (MQ=255)                                        gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGTGGCGCCTTGATCACg                                                                                              <  3:193203/149‑1 (MQ=255)                                        gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                                              <  6:75000/149‑1 (MQ=255)                                        gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                                              <  1:13872/149‑1 (MQ=255)                                         ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                                                                             >  4:293772/1‑149 (MQ=255)                                                  aTTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                                    >  1:28861/1‑149 (MQ=255)                                                      aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                                                  >  2:191200/1‑147 (MQ=255)                                                         tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                                             >  8:48527/1‑149 (MQ=255)                                                           ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                                                           >  8:1284/1‑149 (MQ=255)                                                            gtttaGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCGAGGCACAGGCCCCGGACACGTCCTGCT‑GCAGCGCCTCGTCCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                                                          <  8:370311/146‑1 (MQ=255)                                                                  gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                                                                    <  2:43261/149‑1 (MQ=255)                                                                   tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                                                   <  6:386563/149‑1 (MQ=255)                                                                    ccAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                                                   >  8:318979/1‑148 (MQ=255)                                                                      aGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAc                                                                <  8:8161/149‑1 (MQ=255)                                                                        ctctGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc                                                              <  7:1284/149‑1 (MQ=255)                                                                           tGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATATGCCTTGTACTTGGACCCg                                                             <  7:334484/147‑1 (MQ=255)                                                                             gTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                         >  7:405423/1‑149 (MQ=255)                                                                                aCCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAcc                                                       >  4:281249/1‑148 (MQ=255)                                                                                  cTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTc                                                     >  3:274915/1‑148 (MQ=255)                                                                                                         gacttgcaTTCGTGAATCCAGGCACAGGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgt                              >  1:5488/8‑149 (MQ=255)                                                                                                            ttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctc                          >  1:415909/5‑149 (MQ=255)                                                                                                              gcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                                                <  6:361138/91‑1 (MQ=255)                                                                                                              gcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                                                >  5:361138/3‑93 (MQ=255)                                                                                                              gcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcg                         <  2:101076/146‑1 (MQ=255)                                                                                                               caTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcgtc                       <  2:415909/148‑1 (MQ=255)                                                                                                                             aggcacaGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGc          >  5:411823/7‑149 (MQ=255)                                                                                                                                     gCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGtttt   >  8:21846/1‑148 (MQ=255)                                                                                                                                     gCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTc  <  4:127353/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                          TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCT‑CC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTC  >  NC_002947/4741135‑4741413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑