Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,292	C→T	intergenic (+529/+224)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,292	0	C	T	100.0%	115.7 / NA	34	intergenic (+529/+224)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/15);  total (19/15)

CGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCT‑CC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTC  >  NC_002947/4741161‑4741413                                                                                                                                       |                                                                                                                          cgcgGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTACAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAgcgg                                                                                                           >  3:37814/1‑149 (MQ=255)  gcggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAgcggc                                                                                                          >  1:101076/1‑149 (MQ=255)        tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                                                     >  4:44637/1‑148 (MQ=255)        tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                                                     >  3:188835/1‑148 (MQ=255)        tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                                                     >  3:182147/1‑148 (MQ=255)             ggCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc                                                                                               <  8:27702/149‑1 (MQ=255)              tccATTGCTTATTGAACTGTGTATATGTCCAGCTCTGTTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                                              <  8:274226/148‑1 (MQ=255)              gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGTGGCGCCTTGATCACg                                                                                              <  3:193203/149‑1 (MQ=255)              gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                                              <  1:13872/149‑1 (MQ=255)              gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                                              <  6:75000/149‑1 (MQ=255)               ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                                                                             >  4:293772/1‑149 (MQ=255)                        aTTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                                    >  1:28861/1‑149 (MQ=255)                            aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                                                  >  2:191200/1‑147 (MQ=255)                               tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                                             >  8:48527/1‑149 (MQ=255)                                 ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                                                           >  8:1284/1‑149 (MQ=255)                                  gtttaGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCGAGGCACAGGCCCCGGACACGTCCTGCT‑GCAGCGCCTCGTCCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                                                          <  8:370311/146‑1 (MQ=255)                                        gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                                                                    <  2:43261/149‑1 (MQ=255)                                         tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                                                   <  6:386563/149‑1 (MQ=255)                                          ccAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                                                   >  8:318979/1‑148 (MQ=255)                                            aGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAc                                                                <  8:8161/149‑1 (MQ=255)                                              ctctGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc                                                              <  7:1284/149‑1 (MQ=255)                                                 tGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATATGCCTTGTACTTGGACCCg                                                             <  7:334484/147‑1 (MQ=255)                                                   gTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                         >  7:405423/1‑149 (MQ=255)                                                      aCCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAcc                                                       >  4:281249/1‑148 (MQ=255)                                                        cTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTc                                                     >  3:274915/1‑148 (MQ=255)                                                                               gacttgcaTTCGTGAATCCAGGCACAGGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgt                              >  1:5488/8‑149 (MQ=255)                                                                                  ttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctc                          >  1:415909/5‑149 (MQ=255)                                                                                    gcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                                                <  6:361138/91‑1 (MQ=255)                                                                                    gcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                                                >  5:361138/3‑93 (MQ=255)                                                                                    gcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcg                         <  2:101076/146‑1 (MQ=255)                                                                                     caTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcgtc                       <  2:415909/148‑1 (MQ=255)                                                                                                   aggcacaGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGc          >  5:411823/7‑149 (MQ=255)                                                                                                           gCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGtttt   >  8:21846/1‑148 (MQ=255)                                                                                                           gCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTc  <  4:127353/149‑1 (MQ=255)                                                                                                                                       |                                                                                                                          CGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCT‑CC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTC  >  NC_002947/4741161‑4741413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑