Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	157.1 / NA	46	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/19);  total (27/19)

AGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAA‑GGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAAC  >  NC_002947/499059‑499344                                                                                                                                                  |                                                                                                                                               aGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCa                                                                                                                                            >  3:350568/1‑149 (MQ=255)     cATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGcc                                                                                                                                        >  8:147996/1‑149 (MQ=255)            atctacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAc                                                                                                                                 <  7:114242/145‑1 (MQ=255)            aCCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAc                                                                                                                                 <  7:37536/149‑1 (MQ=255)               cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACggg                                                                                                                              >  8:96192/1‑149 (MQ=255)                acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggc                                                                                                                             <  6:308249/149‑1 (MQ=255)                acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggc                                                                                                                             >  5:308249/1‑149 (MQ=255)                 caAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAc                                                                                                                                 >  8:114242/1‑144 (MQ=255)                        cGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGgcctggcctg                                                                                                                     <  4:327246/149‑1 (MQ=255)                           cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcct                                                                                                                           >  3:124419/1‑140 (MQ=255)                           cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcct                                                                                                                           <  4:124419/140‑1 (MQ=255)                                gCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCAATGGAGCACAAGCTGATCTGATTGCGACATACGGTTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGGGGGCATGCCCGCGAACGGGCCTGGCCTGCTGAtctc                                                                                                             >  5:409650/1‑149 (MQ=255)                                   gAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCAAGCCCGCCCACCGGCCCGGCCGGCGGctatctat                                                                                                          >  7:32098/1‑149 (MQ=255)                                    aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATg                                                                                                         >  7:98971/1‑149 (MQ=255)                                      gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGc                                                                                                       <  7:99490/149‑1 (MQ=255)                                         gAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGAt                                                                                                                >  1:252901/1‑137 (MQ=255)                                         gAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGAt                                                                                                                <  2:252901/137‑1 (MQ=255)                                                aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                                              <  7:147996/148‑1 (MQ=255)                                                  cTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctc                                                                                           >  7:25151/1‑149 (MQ=255)                                                      cTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGgcctggcctg                                                                                                                     <  2:303636/119‑1 (MQ=255)                                                      cTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGAATGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGgcctggcctg                                                                                                                     >  1:303636/1‑119 (MQ=255)                                                         gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                    <  4:60772/149‑1 (MQ=255)                                                           ggAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGCTCTCTATGGCTGAGCCGGCCTCTTCGCggg                                                                                   >  8:301262/1‑148 (MQ=255)                                                           ggAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCggg                                                                                   >  3:111068/1‑148 (MQ=255)                                                               cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGc                                                                              <  8:25151/149‑1 (MQ=255)                                                                 ggTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGcc                                                                             <  8:218157/148‑1 (MQ=255)                                                                 ggTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGcc                                                                             >  7:218157/1‑148 (MQ=255)                                                                   tGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTACCGGGCATGccccc                                                                          >  7:31302/1‑147 (MQ=255)                                                                   tGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACAGGTGCCCG‑AAACTTGCCGAAGCCTATGGGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGCGCCGGCCTCTTCGCGGGCATGCCCGc                                                                          <  6:123934/149‑1 (MQ=255)                                                                          cGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCGGCGGATCTCTATGGCTGAGCCGGCCTCGTCGCGGGCCTGCCCGCTCCca                                                                     >  7:178393/1‑147 (MQ=255)                                                                             cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTATATTGCTGAGCCGGCCTCTTAGCGGGCATGCCCGCTCCCACACGt                                                                >  7:236917/1‑149 (MQ=255)                                                                                         gCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCACTTCGCGGGCAAGCCCGCGCCCACAGGTTTCGTGGTTGGc                                                    >  6:355770/1‑149 (MQ=255)                                                                                           ttatcttatTGCTACATACGGG‑GCCCGTAAACTTGCCTAAGCCTTTGTGGGTGGGGGATTGCCGGCTAAGGGGCCTGGCGTGCTTATGTCTATGGCTGAGCGGGCGTCTTCGGGGGCTTGCCCGCTCCCACAGGTTTCGTGGTTGGCTc                                                  <  6:174182/147‑1 (MQ=255)                                                                                            gatctgatTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCCTGCCCGCGAAACGGCCAGGCCGGCGGGTCTCTATGGCTGTGCCGGCCTCATCGCGGGCCTGCCCGCACCCCCACGGTTCGGGGGTGGCtac                                                 >  3:89767/1‑147 (MQ=255)                                                                                                 gatTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAA‑gg                                           >  5:101317/1‑149 (MQ=255)                                                                                                     gCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCGGAGCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAA‑GGGCAt                                       >  1:292958/1‑149 (MQ=255)                                                                                                                   cccG‑AAACTTGCCGAAGCCTATGTGGGAGCGGGCATTCCCGCGCACCGGGCGGGCCGGCGGATATCTATGGCTGAGCCGGCCGCTTAGCGGGCAATCCCGCTCCCCCAGGGTTGGTGGTTTGGGCGGAAGGGGCATT‑CAATACCGCCa                          >  2:30141/1‑148 (MQ=255)                                                                                                                               ccGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAA‑GGGCATTCCAATCCCGCCATCCTTGTAggg               <  7:96192/148‑1 (MQ=255)                                                                                                                               ccGAAGCCTATGTGGGAGCGGGCAATCCCCCGAAACGGCCGGGCCGGCTGATATGTATTGCTGAGCCGGCCGCATAGCGGGCATTCCCCCGACCACACGTTTAGTGGTTGGGTCGCAA‑AGGCATTGCAAAACCCCCAAACATGTAgggg              >  2:23847/1‑148 (MQ=255)                                                                                                                                      cTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAA‑GGGCATTCCAATCCCGCCATCCTTGTAGGGCGATccc        >  5:302151/1‑148 (MQ=255)                                                                                                                                        aTGTGGGAGCGGGCATGCCCGCTAACGGGCCGGGCCTGCTGATCTCTATGGCGGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAA‑GGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTac     <  1:30141/149‑1 (MQ=255)                                                                                                                                         tgtgGGAGGGGGCATGCCCGGGAACGGGCCTGGCCTGCTGATTTCTTTGGGTGAGCGGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAA‑GGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTa      <  8:98971/147‑1 (MQ=255)                                                                                                                                         tgtgGGAGGGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAA‑GGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTa      <  4:350568/147‑1 (MQ=255)                                                                                                                                            gggAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGGCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAA‑GGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTacaac  >  7:111981/1‑148 (MQ=255)                                                                                                                                               aGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAA‑GGGCATTCCAATccc                              <  4:91719/117‑1 (MQ=255)                                                                                                                                               aGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAA‑GGGCATTCCAATccc                              >  3:91719/1‑117 (MQ=255)                                                                                                                                                  |                                                                                                                                               AGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCG‑AAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAA‑GGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAAC  >  NC_002947/499059‑499344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑