| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 97.4% | 109.3 / ‑3.6 | 38 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (14/23); total (14/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 100.0% | 112.8 / NA | 38 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (14/24); total (14/24) | |||||||||||
CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGC‑‑‑ACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGG‑‑‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATG > NC_002947/1499360‑1499641 || cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGC‑‑‑ACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CAcg‑c < 2:37615/149‑1 (MQ=37) gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCCTTAACTCAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CAc < 1:137929/149‑1 (MQ=21) cTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGC‑‑‑ACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CAcg‑ccc < 3:143505/149‑2 (MQ=37) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGC‑‑‑ACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTcc > 7:342386/1‑149 (MQ=25) cGGCCATTCAGAGCCTGAACGAAGCCTTGGC‑‑‑ACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CAcg‑cccg > 5:399210/1‑133 (MQ=25) cGGCCATTCAGAGCCTGAACGAAGCCTTGGC‑‑‑ACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CAcg‑cccg < 6:399210/135‑3 (MQ=25) ggCCATTCAGAGCCTGAACGAAGCCTTGGC‑‑‑ACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CAcg‑cccgctc < 7:376523/137‑6 (MQ=21) gCCATTCAGAGCCTGAACGAAGCCTTGGC‑‑‑ACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGAtt < 5:314660/149‑1 (MQ=21) cATTCATAGCCTGAAGGAAGCCTTGGC‑‑‑ACCACAGCTGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CAGGCCCGCTCCCACAGACTGATTGc < 6:115743/149‑1 (MQ=18) agagCCTGAACGAAGCCTTGGC‑‑‑ACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCAGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CAcg‑cccgct < 1:311837/128‑5 (MQ=21) cTGAACGAAGCCTTGGC‑‑‑ACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg > 7:321540/1‑149 (MQ=21) aaCGAAGCCTTGGC‑‑‑ACCACACCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGTGc < 2:63656/148‑1 (MQ=21) gC‑‑‑ACCACAGCAGGACTGAGGCTGCCAACCGGTGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGt < 3:133593/149‑1 (MQ=21) ccacAGCAGGACTGAGGCTGCAAAAGGGGGGCTGCGGGCGTGCCCGCTAAACTGGCGCCGCGGTGCAGGGCACGGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc < 4:414339/149‑1 (MQ=21) acaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACgg > 5:427971/1‑149 (MQ=21) aGGCGGCCAAACGGGGGGAGCGGGCGGCCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 3:179131/147‑1 (MQ=21) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 2:256361/148‑1 (MQ=21) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc < 8:342386/149‑1 (MQ=21) gggAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGaa < 3:310004/149‑1 (MQ=25) gCCCGCCAAACCGGCGGCGGGGTGCCCGGCCCGGGGTTTGGGGGTGTG‑GCGGGGCCCCCC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAcccct < 2:194897/149‑2 (MQ=255) aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 6:427971/149‑1 (MQ=37) aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 8:321540/149‑1 (MQ=37) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa > 3:346028/1‑149 (MQ=37) gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa > 6:292970/1‑90 (MQ=17) gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa < 5:292970/90‑1 (MQ=17) gccgcGGTGCATGGCACGGGCTTTGCCGGTGTTCGGGGG‑‑‑AACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg < 1:399416/149‑1 (MQ=255) ccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 5:392424/1‑148 (MQ=37) gcaccGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACg > 8:382001/5‑149 (MQ=38) accGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg > 2:339501/3‑126 (MQ=38) accGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg < 1:339501/124‑1 (MQ=38) accGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc < 4:346028/147‑1 (MQ=38) accGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc > 1:207440/3‑149 (MQ=38) ccGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcc > 3:82604/2‑149 (MQ=39) cGGCTTTGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc < 6:392424/149‑1 (MQ=39) tttGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc > 5:429184/1‑94 (MQ=37) tttGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc < 6:429184/94‑1 (MQ=37) ttGCCGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActt > 1:212845/1‑149 (MQ=255) ccGGTGTTCGCGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtctt > 2:67010/1‑149 (MQ=255) ttttcgcGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt < 1:369690/143‑1 (MQ=255) tgttcgcGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt < 7:159640/143‑1 (MQ=255) ttcgcGGG‑‑‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATg < 4:82604/144‑1 (MQ=255) || CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGC‑‑‑ACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGG‑‑‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATG > NC_002947/1499360‑1499641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |