Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,932,640	+C	intergenic (‑107/+43)	rluA ← / ← minE	bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,932,638	1	.	C	100.0%	188.1 / NA	56	intergenic (‑105/+45)	rluA/minE	bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (30/26);  total (30/26)

ATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCA  >  NC_002947/1932490‑1932774                                                                                                                                                      |                                                                                                                                          aTGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGTGCGGGCTTg                                                                                                                                            >  3:118581/1‑149 (MQ=255)          tCTTCGAAGAGCATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAg                                                                                                                                   <  5:335573/149‑1 (MQ=255)              cGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAgg                                                                                                                                  <  6:164271/146‑1 (MQ=255)                    ggATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAgg                                                                                                                         <  1:14624/149‑1 (MQ=255)                          ggACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAAAAAGGATATGCGTCAACAGTAGGAGCGGGCTTGCCCGCCAAGGGCTGCCAGGCAGccc                                                                                                                   >  1:148618/1‑149 (MQ=38)                                  gACAGCGGCATTGCAGGTCTCACCAAAATCGCATTACACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTg                                                                                                           <  8:218827/148‑1 (MQ=37)                                     aGCGGCATTGCAGGTCTCACGAAAATAGCAGTAACCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGa                                                                                                       <  7:350001/149‑1 (MQ=255)                                     aGCGCCTTTGCTGGTCTCACGAAAATTGCATAAACCGGTGGGCCCGCTTGGCGGCCCTTCGCGGCCACCCCGGAGCCTCAAACGTATATGCGACCCCTTTTGGTGGGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGa                                                                                                       <  2:353263/149‑1 (MQ=255)                                        ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAg                                                                                                                          <  8:261706/128‑1 (MQ=255)                                        ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAg                                                                                                                          >  7:261706/1‑128 (MQ=255)                                        ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGc                                                                                                                     >  6:100990/1‑133 (MQ=255)                                        ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGc                                                                                                                     <  5:100990/133‑1 (MQ=255)                                                 ggTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGAt                                                                                             >  8:178929/1‑147 (MQ=255)                                                    gtatggAAATTAGCTTTAAACGCTGGGGGGGCTTTGCGGCGATTCGCGGGCAAGGCCGCTCTTATTTAGGATATGGTTCCCCTTTAGGAGGGGGCTTGCCCGCGAAGGGCTGCAAGGCAGGCCCA‑AATTGTCGACTAGGCGGATCAGc                                                                                         <  8:116481/143‑1 (MQ=255)                                                     tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGa                                                                                       >  5:347344/1‑149 (MQ=255)                                                       aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTc                                                                                                         >  6:323725/1‑129 (MQ=255)                                                       aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTc                                                                                                         <  5:323725/129‑1 (MQ=255)                                                       aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGc                                                                                         >  7:116481/1‑145 (MQ=255)                                                       aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCGGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATc                                                                                     >  8:82013/1‑149 (MQ=255)                                                               aGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAggg                                                                             >  5:102383/1‑149 (MQ=255)                                                               aGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGATATGCGTGCCCTGTAGGAGCGGGCTTGCCCGCGGAGGGCTGCAAGGCAGCCCCA‑AATTGTAGACTAAGCGGATCAGCGCTACGGCAggg                                                                             >  8:6333/1‑149 (MQ=37)                                                                  aGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGa                                                                          >  5:163495/1‑149 (MQ=255)                                                                   gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGAt                                                                         <  7:6333/149‑1 (MQ=255)                                                                    tAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg                                                                        >  5:137774/1‑149 (MQ=255)                                                                       aCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc                                                                     <  8:41547/149‑1 (MQ=255)                                                                        cGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGTGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCa                                                                    <  4:118581/149‑1 (MQ=255)                                                                         gCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCCAGGCCGCCCCA‑AATTGTCGACTACGCGGATCCGCGGTCCGGGAGGGTGATGTTCAg                                                                   >  5:210499/1‑149 (MQ=37)                                                                            ggggCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGTGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTc                                                                <  3:154926/149‑1 (MQ=255)                                                                                    tttGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCg                                                        >  1:258413/1‑149 (MQ=255)                                                                                      tGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGa                                                       <  6:137774/148‑1 (MQ=255)                                                                                        cGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagca                                                    <  6:347344/149‑1 (MQ=255)                                                                                            ccTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTg                                                <  7:178929/149‑1 (MQ=255)                                                                                                 gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTg                                           >  8:93379/1‑149 (MQ=255)                                                                                                          cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTc                                                                                                         <  1:48413/78‑1 (MQ=255)                                                                                                          cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTc                                                                                                         >  2:48413/1‑78 (MQ=255)                                                                                                             gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg                                                                        >  6:130409/1‑108 (MQ=255)                                                                                                             gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg                                                                        <  5:130409/108‑1 (MQ=255)                                                                                                                  tACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat                          >  8:78617/1‑149 (MQ=255)                                                                                                                   aCAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCt                                                                                                                               <  8:50441/48‑1 (MQ=18)                                                                                                                   aCAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCt                                                                                                                               >  7:50441/1‑48 (MQ=18)                                                                                                                   aCAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg                         >  6:248560/1‑149 (MQ=255)                                                                                                                    cAACGGAAAGGCGTCCCCTGTAGGAGGGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc                        <  5:19209/149‑1 (MQ=39)                                                                                                                    cAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat                          <  7:82013/147‑1 (MQ=255)                                                                                                                     aaaGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca                       >  6:267826/1‑149 (MQ=255)                                                                                                                     aaaGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca                       >  6:225732/1‑149 (MQ=255)                                                                                                                             tGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCg               >  6:189931/1‑149 (MQ=255)                                                                                                                                    ccTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa        >  8:328559/1‑149 (MQ=255)                                                                                                                                    ccTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa        >  6:227549/1‑149 (MQ=255)                                                                                                                                     cTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat                            >  6:4662/1‑128 (MQ=255)                                                                                                                                     cTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat                            <  5:4662/128‑1 (MQ=255)                                                                                                                                      tGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAGAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc           >  1:73658/1‑145 (MQ=255)                                                                                                                                      tGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc           <  2:73658/144‑1 (MQ=255)                                                                                                                                        tAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGt     <  7:93379/148‑1 (MQ=255)                                                                                                                                          ggAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca  >  1:25100/1‑149 (MQ=255)                                                                                                                                           gAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca  >  6:388477/1‑148 (MQ=255)                                                                                                                                                 gCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc                        <  2:7479/120‑1 (MQ=255)                                                                                                                                                 gCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc                        >  1:7479/1‑120 (MQ=255)                                                                                                                                                      |                                                                                                                                          ATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCA  >  NC_002947/1932490‑1932774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑