| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 3,845,705 | +G | intergenic (‑72/+26) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 97.9% | 160.3 / ‑3.2 | 48 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (30/17); total (30/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
GGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGC > NC_002947/3845556‑3845844 | ggCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTc‑gc < 5:375096/148‑1 (MQ=255) ctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCGGACTTCATTGATTGTTATTGTTGTCCTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑gggct < 2:99338/148‑4 (MQ=255) tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGAt < 8:175305/148‑1 (MQ=255) tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGAt < 1:364007/148‑1 (MQ=255) ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCa > 6:318847/1‑149 (MQ=255) gCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTc‑gc < 1:235870/137‑1 (MQ=255) gCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTc‑gc > 2:235870/1‑137 (MQ=255) tggcTTGCAGAGCCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAg > 2:244546/4‑149 (MQ=255) tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCa < 3:34950/148‑1 (MQ=255) ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGAt > 5:63709/1‑135 (MQ=255) ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGAt < 6:63709/135‑1 (MQ=255) ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCAc > 2:44159/1‑149 (MQ=255) ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCAAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCAc > 4:331922/1‑149 (MQ=255) ccggCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGCAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGa < 8:243471/146‑1 (MQ=255) cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TGGCTGACGATGGATCa < 5:318847/146‑1 (MQ=255) cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TGGCTGACGATGGATCa < 1:293180/146‑1 (MQ=255) aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGccc > 7:224077/1‑149 (MQ=255) gCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCcgc > 3:310823/1‑149 (MQ=255) aTCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTTATACATCG‑TCAGCCACGCTTGCTCAGCCCGcccc > 1:388895/1‑149 (MQ=255) aTCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGATGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGcc > 1:344161/1‑149 (MQ=255) gcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCGATCAGCCACGATGGATCAGCCCGCGCCCTGc < 1:35719/149‑1 (MQ=255) cAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCa < 1:44159/149‑1 (MQ=255) cAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCa < 7:279758/149‑1 (MQ=255) aGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCTGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAg > 1:313716/1‑149 (MQ=255) gCCTACTTCTTTGATGTTTTTTGTTGTCATCCCGGTCGCTCTGTCTGAACCGTTGCGCCAGACAAAGAGCTGCAGCACGGGCATAAAAGCCGTGTTGC‑GCGGGCTGATCCATCG‑CCAGCCACTATGCATCAGCCCGCGCCCTGCCAGGc < 6:322794/149‑1 (MQ=255) ttgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcagc > 3:83590/1‑148 (MQ=255) gttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCaa > 7:23564/1‑148 (MQ=255) attgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg > 8:6543/1‑149 (MQ=255) attgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCAACG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg > 3:167043/1‑149 (MQ=255) ttgttgTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAAc > 7:312671/1‑145 (MQ=255) ttgttgTCATCGCGGTCTCTCTGCCTGACGCGTGGCGCCACACTAAGCGCTGGATGACGGGAAGAAACGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg < 7:157336/148‑1 (MQ=255) ttgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtggt > 7:216576/1‑149 (MQ=255) ttcctCGCGGTCTCGCTGTGTTATGGGTGGCCCCTCAATATGAGCTGTTTTAGGGGCTTAAAACCCGTTTTTCGGG‑GGCTTACCCATCG‑CCCCCAACTTTGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTc < 8:23564/144‑1 (MQ=255) gtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACTATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTc < 1:244546/148‑1 (MQ=255) gtCATCGCGGTCTCCCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGTACGCAGCAACAGGTGGTc > 5:178192/1‑148 (MQ=255) tctctctcTGTCTGATGCGTTGCGCCATACTAAGAGGTGTATGACGGGCAGAAAAGAGGTGTTTC‑GCGGGCTGATCCTGGG‑TCTGCCACGATGTATCTGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGgc < 2:315419/146‑1 (MQ=255) tctctGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCAAGG‑TCAGCCACGATGAGTCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGgc > 1:315419/1‑144 (MQ=255) tgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAg > 4:16216/1‑149 (MQ=255) aGCATTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCCGGCGCCGAGCCGACAGc > 6:72924/1‑147 (MQ=255) gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATAAGCCCGCGCCCGGCCAGGCCGGTGCACGCAGCAAAACGTGGTCAAGCAGGCCCCGAGCCGCCACCGAGAGCgtgt > 1:375333/1‑149 (MQ=255) ccAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCTCCCTGCCAGGCCGGTGCACGCAGCAACACGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGtgtg > 7:208403/1‑149 (MQ=255) aGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATACATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGGGGTCAAGCCCGCGCCGCGCGGCCAGCGCGCGCGTGTGcc > 8:218000/1‑149 (MQ=255) agagCGGGATGACGGGCACAAAAGCCGTGTTTC‑GCGGGCTGACCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcgc < 1:128173/149‑1 (MQ=255) gATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGTACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCa < 6:178192/149‑1 (MQ=255) gATGACGGGCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg > 7:11102/1‑147 (MQ=255) gCAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGACAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACg > 7:243383/1‑148 (MQ=255) cAGAAAAGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATAAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGaa < 8:216576/149‑1 (MQ=255) aaaaGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAtt > 3:223248/1‑148 (MQ=255) aaaaGACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTc > 2:272092/1‑149 (MQ=255) gACGTGTTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCAcc > 8:264579/1‑148 (MQ=255) gtgtTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTc > 7:163648/1‑121 (MQ=255) gtTTC‑GCGGGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCGCCAAgcgc > 3:323273/1‑149 (MQ=255) | GGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GGCTGATCCATCG‑TCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGC > NC_002947/3845556‑3845844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |