Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,843,370	G→T	T475N (ACC→AAC)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,843,370	0	G	T	98.0%	164.2 / NA	50	T475N (ACC→AAC)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (25/24);  minor base A (1/0);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTGGCAGCGCTTCGGCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCA  >  NC_002947/1843225‑1843514                                                                                                                                                   |                                                                                                                                                 cGGTGGCAGCGCTTGGGCATCGTCATGAGGCTTGGGCAGGTTGAGGCTGATCCAGTACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TGGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTg                                                                                                                                                <  5:84789/148‑1 (MQ=255)    tGGCAGCGCTTCGGCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTgccgc                                                                                                                                            <  5:199017/149‑1 (MQ=255)        aGCGCTTCGGCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCggg                                                                                                                                        >  4:308306/1‑149 (MQ=255)         gcgcTTCGGCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGc                                                                                                                                       >  4:162045/1‑149 (MQ=255)          cgcTTCGGCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCt                                                                                                                                      >  8:130802/1‑149 (MQ=255)                ggCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCgg                                                                                                                                 <  8:93451/148‑1 (MQ=255)                 gCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGc                                                                                                                               <  4:196010/149‑1 (MQ=255)                  cATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGc                                                                                                                               >  3:139871/1‑148 (MQ=255)                      gTCATGAGCCATGGGCAGGTTGACGCTGATCCACAACTCCGAACCTTCGCCAGGGGTACTGTCGACACCCAAC‑TCCCCGCCCATCTGTTCGATCACGCGCAAGGAAATCACCAGACCCACACCCTTGCCGCCGCGCAGGCGGGCCact                                                                                                                           >  8:255832/1‑146 (MQ=255)                         aTGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAg                                                                                                                        <  8:94605/148‑1 (MQ=255)                                   ggCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCAGCTGTTCGATCAGGCGCTTGGAAAGCCCCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGGCAGTTGTCAGccgg                                                                                                             >  4:290412/1‑147 (MQ=255)                                    gCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCGGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGTCTGGCGGGCCAGTGAGTTGTCAGCCTgg                                                                                                            <  7:130802/149‑1 (MQ=255)                                    gCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTgg                                                                                                            >  7:300775/1‑149 (MQ=255)                                     cAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGc                                                                                                           <  4:139871/149‑1 (MQ=255)                                           gAGGCTGATCCAGAACTGCCAACCTTCGCCCCGGCTACTGTCGACACCGATC‑ACCCCGCCCATCTCTTCGATCAGCCGCTTGGAAATCCCCAGCCCCCGACCCAAGCCGCCGGGCAGGCGGGCCAGGGAGTTGACAGCCAGGCTGAAgg                                                                                                     >  8:352166/1‑149 (MQ=255)                                             ggCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGcc                                                                                                   >  6:104506/1‑149 (MQ=255)                                             ggCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGcc                                                                                                   >  3:168784/1‑149 (MQ=255)                                              gCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATCATCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGcc                                                                                                   >  1:90850/1‑149 (MQ=255)                                                      aGAACTGCGAAACTTCGCCCGGGGTACTGTCGACACGGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAgt                                                                                          <  7:255832/149‑1 (MQ=255)                                                       gAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCTGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGc                                                                                                    <  3:84189/138‑1 (MQ=255)                                                       gAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCTGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGc                                                                                                    >  4:84189/1‑138 (MQ=255)                                                        aaCTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAgtgt                                                                                        <  3:308306/149‑1 (MQ=255)                                                         aCTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGtgtg                                                                                       <  5:104506/149‑1 (MQ=255)                                                               aaCCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCCGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGca                                                                                   >  2:312882/1‑147 (MQ=255)                                                                  cTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATc                                                                               >  7:95019/1‑148 (MQ=255)                                                                  cTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATc                                                                               <  8:95019/148‑1 (MQ=255)                                                                      gCCCGGGGTACAGTCGACACCGAGC‑TCGCCGCCCATCTGGTCGCTCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGa                                                                           <  6:216710/148‑1 (MQ=255)                                                                          ggggTCCTGTCGTCACCGATC‑TCGCCGCCCATCTGTTCGTTCAGGCGCTTGGAAATCACGAGCCCCAGACCGTGGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGa                                                                      <  7:352166/149‑1 (MQ=255)                                                                              tACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAgg                                                                  <  3:162045/149‑1 (MQ=255)                                                                                 tGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGcc                                                                >  1:150461/1‑148 (MQ=255)                                                                                     gACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCATCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGc                                                           >  1:95615/1‑149 (MQ=255)                                                                                     gACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGc                                                           >  3:46780/1‑149 (MQ=255)                                                                                     gACACCGAGC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGCCCGTTGCCGCCGGGCGGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGc                                                           <  3:290412/149‑1 (MQ=255)                                                                                        acCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCgg                                                        >  4:120678/1‑149 (MQ=255)                                                                                         ccGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGgt                                                       <  3:120678/149‑1 (MQ=255)                                                                                                  gccgccCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCt                                                  <  2:158553/146‑1 (MQ=255)                                                                                                    cgccCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACg                                             <  4:168784/149‑1 (MQ=255)                                                                                                         aTCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGAt                                        >  8:121573/1‑149 (MQ=255)                                                                                                         aTCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGAACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGAt                                        >  4:30171/1‑149 (MQ=255)                                                                                                                 gATCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACt                                >  6:54965/1‑149 (MQ=255)                                                                                                                  aTCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATgc                                      >  4:351027/1‑142 (MQ=255)                                                                                                                  aTCAGGCGCTTGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATgc                                      <  3:351027/142‑1 (MQ=255)                                                                                                                           ttGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCaa                                  >  7:307308/1‑137 (MQ=255)                                                                                                                           ttGGAAATCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCaa                                  <  8:307308/137‑1 (MQ=255)                                                                                                                               aaaTCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCtttg                  >  3:34156/1‑147 (MQ=255)                                                                                                                               aaaTCACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCg                  <  2:90850/149‑1 (MQ=255)                                                                                                                                   cACCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCt              >  1:321839/1‑149 (MQ=255)                                                                                                                                    aCCAGCCCCAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTc             <  3:254621/149‑1 (MQ=255)                                                                                                                                           ccAGACCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGAACATCCTGAGGTGACAGGCAGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGAGCAGTCTTCGTGCTCGACTTc       >  8:209239/1‑148 (MQ=255)                                                                                                                                               aCCGTTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCa  <  2:95615/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CGGTGGCAGCGCTTCGGCATCGTCATGAGCCTTGGGCAGGTTGAGGCTGATCCAGAACTGCGAACCTTCGCCCGGGGTACTGTCGACACCGATC‑TCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCA  >  NC_002947/1843225‑1843514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑