Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,951,161	A→T	intergenic (‑125/+70)	PP_3486 ← / ← PP_3487	cytochrome c/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,951,161	0	A	T	100.0%	138.2 / NA	42	intergenic (‑125/+70)	PP_3486/PP_3487	cytochrome c/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (24/18);  total (24/18)

TCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCTTATCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCG  >  NC_002947/3951024‑3951302                                                                                                                                           |                                                                                                                                              tCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGCGCGCTGCGCGCTCCTTTCGcgccgc                                                                                                                                      >  7:291998/1‑147 (MQ=255)         gccatgccaAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCg                                                                                                                               <  7:100840/146‑1 (MQ=255)         gccatgccaAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCg                                                                                                                               >  8:100840/1‑146 (MQ=255)                                                       cGGCCTGGCGCGGCAAATGGGCTGCGCAG‑ACCCCCGGCCTGTGCCAGCTCAACCGCACAACGCGGGGCCCCGGGCGCGCGCCTTTCGCCACCAAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCAt                                                                               <  2:89855/148‑1 (MQ=255)                                                        ggCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGCAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGa                                                                             >  3:339421/1‑149 (MQ=255)                                                          ccTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATa                                                                           >  1:370750/1‑149 (MQ=255)                                                             tGCGTCGGTAATGGTCTGGCCATCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTc                                                                        <  3:252153/149‑1 (MQ=38)                                                              gCGTCGCGAAAGGGCTGCGCAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCa                                                                       >  7:287535/1‑149 (MQ=255)                                                                          ggCTGCGCAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAg                                                           >  7:291713/1‑149 (MQ=255)                                                                           gCTGCGCAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAAGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGc                                                          >  8:30102/1‑149 (MQ=255)                                                                             tGCGCAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCgg                                                        >  2:159805/1‑149 (MQ=255)                                                                             tGCGCAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCgg                                                        >  2:275774/1‑149 (MQ=255)                                                                              gcgcAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGc                                                       <  8:291713/149‑1 (MQ=255)                                                                               cgcAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTcccc                                                                                          >  4:214631/1‑113 (MQ=255)                                                                               cgcAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTcccc                                                                                          <  3:214631/113‑1 (MQ=255)                                                                                 cagcagCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTgg                                                    <  1:159805/149‑1 (MQ=255)                                                                                     agcCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCa                                                <  8:287535/149‑1 (MQ=255)                                                                                        cccGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGt                                                  <  8:273325/144‑1 (MQ=255)                                                                                        cccGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGt                                                  >  7:273325/1‑144 (MQ=255)                                                                                         ccGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAt                                                                            <  1:47813/117‑1 (MQ=255)                                                                                         ccGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAt                                                                            >  2:47813/1‑117 (MQ=255)                                                                                          cGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGc                                           >  8:132074/1‑149 (MQ=255)                                                                                          cGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCACTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGc                                           >  1:14748/1‑149 (MQ=255)                                                                                           gg‑CAGTGGCAGCTCAAGCGCATAAAGTGGGTAGCGCTGGGCGCTCCTTTCGCTACGAAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCt                                          <  2:266622/149‑1 (MQ=255)                                                                                                gTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCAt                                      <  7:132074/149‑1 (MQ=255)                                                                                                       cTCAAGCTCAGAAAGATGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCTCTCCCACAAAAGCGGATGCCACAGAGGACAATTCCCCAGGCGGCCCATGATACTCACCGACCGTCCAGCTGCTGGGTCAACAGCTGCATGCGCTgg                               <  7:229492/149‑1 (MQ=255)                                                                                                       cTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGc                                                          <  3:282714/122‑1 (MQ=255)                                                                                                       cTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGc                                                          >  4:282714/1‑122 (MQ=255)                                                                                                       cTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTgg                               >  8:89585/1‑149 (MQ=255)                                                                                                          aaGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGc                             >  6:110591/1‑148 (MQ=255)                                                                                                            gcgcAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAAt                          >  1:162486/1‑149 (MQ=255)                                                                                                                 gAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCGGGGTCAACAGCTGCATGCGCTGGGCAATcgccg                     >  8:242780/1‑149 (MQ=255)                                                                                                                    agagGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTg                  >  4:296485/1‑149 (MQ=255)                                                                                                                     gagGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAAGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTgg                 <  7:30102/149‑1 (MQ=255)                                                                                                                      agGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGc                >  7:342406/1‑149 (MQ=255)                                                                                                                       ggggAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCg               <  5:110591/149‑1 (MQ=255)                                                                                                                         ggAGCGCTGCGCACTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGt             <  2:14748/149‑1 (MQ=255)                                                                                                                                tGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGt             <  7:347969/142‑1 (MQ=255)                                                                                                                                tGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGt             >  8:347969/1‑142 (MQ=255)                                                                                                                                tGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCg      >  8:288791/1‑149 (MQ=255)                                                                                                                                  cgcgcTCCTTTCGCTACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCggg    <  6:312258/149‑1 (MQ=255)                                                                                                                                     gcTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCg  >  5:346861/1‑148 (MQ=255)                                                                                                                                           |                                                                                                                                              TCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGG‑CAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCTTATCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCG  >  NC_002947/3951024‑3951302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑