Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,740,816 T→G intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8160TG98.3% 196.3 / ‑4.4 59intergenic (+53/+700)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (28/30);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTG  >  NC_002947/4740679‑4740947
                                                                                                                                         |                                                                                                                                      
cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCTTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑c                                                                                                                         >  3:321459/1‑149 (MQ=255)
      gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTc                                                                                                                   <  7:293204/149‑1 (MQ=255)
       tACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTcg                                                                                                                  >  6:85527/1‑149 (MQ=255)
        aCAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTcgc                                                                                                                 >  5:83247/1‑149 (MQ=255)
         cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTcgcg                                                                                                                >  7:289156/1‑149 (MQ=255)
              tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCt                                                                                                            <  7:237163/148‑1 (MQ=255)
                 gCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGcc                                                                                                        >  6:353562/1‑149 (MQ=255)
                    gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCcgc                                                                                                     <  5:353562/149‑1 (MQ=255)
                       cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTTCT‑GTGCCGG‑‑‑CCTCTTCGCGGGCAAGGCCGCGaa                                                                                                  <  7:329592/149‑1 (MQ=255)
                       cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGGCAAGGCCGCGaa                                                                                                  <  7:61241/149‑1 (MQ=255)
                       cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGaa                                                                                                  <  3:140930/149‑1 (MQ=255)
                       cccgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGaa                                                                                                  <  2:289205/147‑1 (MQ=255)
                         cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                <  4:134421/149‑1 (MQ=38)
                         cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                <  1:89529/149‑1 (MQ=255)
                          gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAagag                                                                                               <  8:119582/149‑1 (MQ=255)
                          gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTAAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAagag                                                                                               <  8:39835/149‑1 (MQ=255)
                           ccAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAgg                                                                                              <  5:164083/149‑1 (MQ=255)
                              ggtGTACACGGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGGTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCGTTGCCGGCGAAGAGGCCg                                                                                           <  2:138928/147‑1 (MQ=255)
                               cTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTTCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCgg                                                                                          >  6:193208/1‑149 (MQ=255)
                                tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGCAGAGGCCGGc                                                                                         >  6:272202/1‑149 (MQ=255)
                                 gTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGGCAAGGCCGCGAagag                                                                                               <  7:175465/142‑1 (MQ=38)
                                 gTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGGCAAGGCCGCGAagag                                                                                               >  8:175465/1‑142 (MQ=38)
                                   acacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGca                                                                                        >  1:9100/1‑147 (MQ=255)
                                        ggCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGGGACCTATTAGCGGCCTTGCCCGCGAAGAGGCCGGccaaga                                                                                    >  1:129132/1‑144 (MQ=255)
                                               aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCCGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaaa                                                                          >  8:91728/1‑149 (MQ=255)
                                                gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGACACCACAAAAc                                                                         >  7:363516/1‑149 (MQ=255)
                                                gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                         >  7:271004/1‑149 (MQ=255)
                                                  aGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCt                                                                       <  5:85527/149‑1 (MQ=255)
                                                      cgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCACTTCGCGGCCTTGCCCGCGCAGAGGCCGGCCCAGACACCACAAAACCACAgg                                                                   >  7:326078/1‑148 (MQ=255)
                                                       gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCCGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGtt                                                                  <  7:91728/149‑1 (MQ=255)
                                                           tCGTTCCCCTGAAAGCCCGCAAGTACGCTGCAGTAGCCGAACGCACGACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtc                                                              <  8:363516/149‑1 (MQ=255)
                                                                  ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTATTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCGCAGTTTTTGTccgccc                                                       >  8:57438/1‑149 (MQ=255)
                                                                         gACCGAATGTACGCTCCAGTAGCCTAACGCAAGACGGCTGCCCTCGGGCAG‑CTTTTTTTTTTTGTCCTGTGCCGG‑‑‑CCTCTGCGGGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                <  2:9100/149‑1 (MQ=255)
                                                                          aCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                >  4:305151/1‑148 (MQ=255)
                                                                            cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTAGCGGCCTTGCCCGCGCAGAGGCCGGCACAGacac                                                                                 >  3:348265/1‑113 (MQ=255)
                                                                              cAAGTACGCTGCCGTAGCCGAAGGCATGACGGCTGCCCTCGGGCTGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTGCGCGGCCTGGCCCGCCAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGcc                                           <  6:83247/149‑1 (MQ=255)
                                                                              cAAGTAAGCTGAAGTAGCCGAACGCATGAAGGCTGCCGTCGGGAAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCGGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGcc                                           <  6:311764/149‑1 (MQ=255)
                                                                                     gCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGTGCAGCC‑TTTTTTATTTGTCCTTTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                    <  5:88568/149‑1 (MQ=255)
                                                                                      gtGCAGTCCCCGACCCCAAGCAGGCGGCCTTGGGGCAG‑CTTTTTTTTTTTGTCGTGGGCCGG‑‑‑CCTCTGCGCGGCCTGGCCCGCGATGTGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                   <  4:321459/148‑1 (MQ=255)
                                                                                      cggCAGTAGCCGACCGCATGACGGCTGCCCTCGGGAAGCC‑TTTTTTATTTGTCCTGTCCCGG‑‑‑CCGCTTCGCGGCCTTGCCCGCGAAGTGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                   <  8:271004/147‑1 (MQ=255)
                                                                                        gCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCGTGGCCGCGAAGAGGGCGGCCCAGACACCACAAAACCTCAGTTTTTATCCGCCCGCGGACGCAGCCCGTTCAgggg                                 >  7:145212/1‑148 (MQ=255)
                                                                                           gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGCAGAGGCCGGCACAGCCACCACAAAACCTCAGTGTTTGTACGCCCGCTCACGCAGCCCGGTGAGGGTAtt                              >  3:95692/1‑149 (MQ=255)
                                                                                            tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCACTTAGCGGCCTTGGCCGCGAATAGTCCGGGCCAGCCACCACAAAAACGCCCTTTTTATCAGCCCCCTCCCGCCCCCCATTCAGGGGAtt                              >  4:198856/1‑148 (MQ=255)
                                                                                              gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGa                            <  3:305151/148‑1 (MQ=255)
                                                                                              gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCGTGCCCGGGAAGAGGGCGGGGCCGACCCCACAAAACCTCAGGTTTTCTCCGCCCGCTCCCGCAGCCCGTTGAGGGTATTgta                           >  7:137000/1‑147 (MQ=255)
                                                                                               cccaaCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAc                          <  7:57438/146‑1 (MQ=255)
                                                                                               ccGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAc                          >  7:283798/1‑149 (MQ=255)
                                                                                                 gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGACCCCACAAAACCTCAGTTTTTCTCCGCCCGCTGACGCAGCCCGTTCAGGGGTTTGAACgg                        >  1:154106/1‑149 (MQ=255)
                                                                                                      aaaaaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGCGGCCGGCACCGACACCACAAAACCGCAGTTTTTGTCCGCCCGCGCACGCAGCCCTTTCAGGGGATTGAACGGCGCAt                   >  7:28431/5‑149 (MQ=255)
                                                                                                       aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGGCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAtt                              <  7:272231/137‑1 (MQ=255)
                                                                                                        aaaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc                  >  2:82566/3‑148 (MQ=255)
                                                                                                             gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgccc                                                       >  3:34679/1‑106 (MQ=38)
                                                                                                                 cccgagggCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAc        <  1:204343/144‑1 (MQ=255)
                                                                                                                  ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGGCCGGGAAGGGGCCGGCACAGACACCACAAAACCTGAGTTTTTCTGCGCCCGCTCACGCCGCCCGTTCAGGGTATTGAACGGCGCATCCACCCCGAACt       >  8:212879/1‑149 (MQ=255)
                                                                                                                      gggCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt   <  5:272202/149‑1 (MQ=255)
                                                                                                                      gggCAGCC‑TTTTTTATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt   <  8:283798/149‑1 (MQ=255)
                                                                                                                        gtcagcattttttATTTGGCCGGGGCCGC‑‑‑CCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg  <  4:40131/142‑1 (MQ=255)
                                                                                                                                  tttATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt   >  5:357219/1‑138 (MQ=255)
                                                                                                                                  tttATTTGTCCTGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt   <  6:357219/138‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGG‑‑‑CCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTG  >  NC_002947/4740679‑4740947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑