Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,551,996 T→C S409P (TCA→CCA)  PP_4882 → iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,551,9960TC96.9% 206.9 / ‑2.8 64S409P (TCA→CCA) PP_4882iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (32/30);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

GCCCTATCTGCTGCACGCCCTGCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAATCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGATCATCTTCCCGGCCATGGCGGCAGCCTTTGCGCTG  >  NC_002947/5551854‑5552136
                                                                                                                                               |                                                                                                                                            
gCCCTATCTGCTGCACGCCCTGCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCgg                                                                                                                                        >  8:224864/1‑149 (MQ=255)
 cccTATCTGCTGCACGCCCTGCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGt                                                                                                                                       <  3:300313/149‑1 (MQ=255)
        tgctgcACGCCCTGCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCAcc                                                                                                                                >  6:297287/1‑149 (MQ=255)
            gcACGCCCTGCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTACGGGGCCGGCGCTGTACCAGACCCCGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACcgcg                                                                                                                             >  1:304991/1‑149 (MQ=255)
              aCGCCCTGCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCt                                                                                                                           >  5:12736/1‑148 (MQ=255)
                gCCCTGCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGaa                                                                                                                        >  8:183549/1‑149 (MQ=255)
                gCCCTGCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCCGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGaa                                                                                                                        <  6:126115/149‑1 (MQ=255)
                 cccTGCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAAc                                                                                                                       <  5:297287/149‑1 (MQ=255)
                  ccTGCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAAc                                                                                                                       >  8:63146/1‑148 (MQ=255)
                     gCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTg                                                                                                                          >  7:214618/1‑142 (MQ=255)
                     gCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTg                                                                                                                          <  8:214618/142‑1 (MQ=255)
                      cccGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAgg                                                                                                                   <  6:12736/148‑1 (MQ=255)
                      cccGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGc                                                                                                                  >  7:321594/1‑149 (MQ=255)
                        cGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCtt                                                                                                                <  7:183549/149‑1 (MQ=255)
                         ggCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAg                                                                                                                    >  5:329294/1‑144 (MQ=255)
                         ggCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAg                                                                                                                    <  6:329294/144‑1 (MQ=255)
                               gTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGAc                                                                                                         >  5:121573/1‑149 (MQ=255)
                                   ttGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTg                                                                                                     >  2:301729/1‑149 (MQ=255)
                                        cTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGgaaga                                                                                                <  7:63146/149‑1 (MQ=255)
                                           aCGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGgaagaa                                                                                               <  6:99823/147‑1 (MQ=255)
                                           aCGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGgaagaa                                                                                               <  8:321594/147‑1 (MQ=255)
                                                ggTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCcgcgc                                                                                        >  1:23220/1‑149 (MQ=255)
                                                   gtTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAATCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCa                                                                                     >  2:334597/1‑149 (MQ=255)
                                                   gtTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTgg                                                                                                    <  3:339332/134‑1 (MQ=255)
                                                   gtTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTgg                                                                                                    >  4:339332/1‑134 (MQ=255)
                                                   gtTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCa                                                                                     >  4:50067/1‑149 (MQ=255)
                                                    ttcttcGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCAc                                                                                    >  5:243430/1‑149 (MQ=255)
                                                    ttcttcGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCAc                                                                                    >  5:359695/1‑149 (MQ=255)
                                                       ttcGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGCCGCTGGAAGAAGCCGCGCGCACCCt                                                                                 >  2:30723/1‑149 (MQ=255)
                                                          gCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCTCGCGCACCCTTgg                                                                              >  8:263783/1‑149 (MQ=255)
                                                          gCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTg                                                                               <  6:243430/148‑1 (MQ=255)
                                                           cccTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGgc                                                                             <  8:358581/149‑1 (MQ=255)
                                                            ccTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGgcg                                                                            >  7:362134/1‑149 (MQ=255)
                                                                  cTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGagca                                                                      <  3:245357/149‑1 (MQ=255)
                                                                            ggCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGgcgc                                                                           <  3:5393/135‑1 (MQ=255)
                                                                            ggCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGgcgc                                                                           >  4:5393/1‑135 (MQ=255)
                                                                               gcTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGaa                                                                                                                        >  3:281787/1‑87 (MQ=255)
                                                                               gcTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGaa                                                                                                                        <  4:281787/87‑1 (MQ=255)
                                                                                    aCCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGc                                                     >  5:214896/1‑149 (MQ=255)
                                                                                     ccAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGc                                                     <  4:266489/148‑1 (MQ=255)
                                                                                       aGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCgg                                                  <  1:301729/149‑1 (MQ=255)
                                                                                        gACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCggg                                                 <  6:214896/149‑1 (MQ=255)
                                                                                         aCCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGt                                                <  2:365009/149‑1 (MQ=255)
                                                                                         aCCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGt                                                >  6:329495/1‑149 (MQ=255)
                                                                                          ccACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAATCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTg                                               <  1:334597/149‑1 (MQ=255)
                                                                                           cACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGt                                                >  3:273347/1‑147 (MQ=255)
                                                                                           cACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGt                                                <  4:273347/147‑1 (MQ=255)
                                                                                           cACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGa                                              >  1:267431/1‑149 (MQ=255)
                                                                                            aCGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGAc                                             <  7:224864/149‑1 (MQ=255)
                                                                                                ccTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTg                                         <  6:121573/149‑1 (MQ=255)
                                                                                                     ctgctgTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGat                                    >  6:327645/1‑149 (MQ=255)
                                                                                                           ttGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGatcatc                                <  8:352752/147‑1 (MQ=255)
                                                                                                             ggCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCg                                                   <  7:31888/126‑1 (MQ=255)
                                                                                                             ggCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCg                                                   >  8:31888/1‑126 (MQ=255)
                                                                                                             ggCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGATCATCTTc                             <  2:304991/148‑1 (MQ=255)
                                                                                                                cTATGCGCTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGATCATCTTCCCgg                         >  6:23145/1‑149 (MQ=255)
                                                                                                                     cgcTGCTGTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGATCATCTTCCCGGCCAt                     <  5:23145/148‑1 (MQ=255)
                                                                                                                       ctgctgTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGATCATCTTCCCGGCCATggc                  >  8:295823/1‑149 (MQ=255)
                                                                                                                       ctgctgTTCCTGCCGTTGGCGCAACCACCGATGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGATCATCTTCCCGGCCATggc                  >  1:306381/1‑149 (MQ=255)
                                                                                                                        tgctgTTCCTGCCGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGATCATCTTCCCGGCCATggcg                 >  5:72960/1‑149 (MQ=255)
                                                                                                                           tgTTCCTGCCGTTGGCGCAACCACCGATGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGATCATCTTCCCGGCCATGGCGGCa              <  2:306381/149‑1 (MQ=255)
                                                                                                                                   ccGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGATCATCTTCCCGGCCATGGCGGCAGCCTTTgc      <  7:263783/149‑1 (MQ=255)
                                                                                                                                   ccGTTGGCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGATCATCTTCCCGGCCATGGCGGCAGCCTTTgc      >  5:397587/1‑149 (MQ=255)
                                                                                                                                        ggCGCAACCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGATCATCTTCCCGGCCATGGCGGCAGCCTTTGCGCTg  <  8:362134/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                            
GCCCTATCTGCTGCACGCCCTGCCCGGCCTGGTGATTGCCCTGACGTTGGTGTTCTTCGCCCTGCACTAC‑GTGCCGGCGCTGTACCAGACCACGGCCTTGCTGCTGTTGGCCTATGCGCTGCTGTTCCTGCCGTTGGCGCAATCACCGGTGCGCACCGCGCTGAACAAGGCTTCGCCGACGCTGGAAGAAGCCGCGCGCACCCTTGGCGCGAGCAGCTTTGCCGCGTTCTGCCGGGTGACCTTGCCGATCATCTTCCCGGCCATGGCGGCAGCCTTTGCGCTG  >  NC_002947/5551854‑5552136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑