Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	6,013,885	T→C	intergenic (+90/+20)	xpt → / ← PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,013,885	0	T	C	100.0%	115.0 / NA	35	intergenic (+90/+20)	xpt/PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/17);  total (18/17)

CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAA  >  NC_002947/6013745‑6014030                                                                                                                                              |                                                                                                                                                  cTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATgg                                                                                                                                           <  2:227025/149‑1 (MQ=255)      ccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGc                                                                                                                                       >  1:14210/1‑148 (MQ=255)         ggTGAAGTCGCTGGAAGGTGGTGTGGCCAGCTTCATCGAATAACCGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGCGCAGCACAGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAAcc                                                                                                                                   <  3:81789/149‑1 (MQ=255)                  ccaggAAGGTGGTGGGGTCAGCTTCAACGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACACGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTAt                                                                                                                            <  5:285950/144‑1 (MQ=38)                                               aaTAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGCACGCTGT‑GCCGTACCGGTGGGAGCCGGCGTGCCCGCGAATGGCCGCAACCCTTTTATATTGTTGCCGCCAGCCCCGCCACCAACAAgc                                                                                             >  5:286341/1‑149 (MQ=37)                                                         acacCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAg                                                                                   <  3:54255/149‑1 (MQ=255)                                                             cTGCTTCGCGGGCACCCCCGCACCCACAGGTGCAGCACAGGTATAGAACGCTGT‑GCCGTACCTGTGGGAGTGGGCGTGCCCGCGAATGGCCGCAACCCATTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCCCGGGTAAAGatct                                                                               >  2:12679/1‑149 (MQ=255)                                                                   cgggggCCCGCCCGCCCCCACCGGTCCACAACAGGTCTCCAACGCTGT‑GCCGTACGTGGGGGTGGGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCttt                                                                          <  1:382726/145‑1 (MQ=255)                                                                    gcgGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTaa                                                                        <  7:60088/149‑1 (MQ=255)                                                                                     cacaGGTGCAGCACAGGTCTCGAACGCTGTGGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                                                        >  1:231942/1‑149 (MQ=255)                                                                                     cacaGGTACGGCACAGCGTTCGAGACCTGT‑GCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                                                        >  7:29983/1‑148 (MQ=21)                                                                                      acaGGTGCAGCACAGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc                                                       >  6:397803/1‑148 (MQ=255)                                                                                      acaGGTGCAGCACAGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGAGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCACGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCATACGGCTGcccc                                                       >  3:55622/1‑148 (MQ=255)                                                                                      acaGGTACGGCACAGCGTTCGAGACCTGT‑GCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc                                                          <  8:319914/145‑1 (MQ=21)                                                                                      acaGGTACGGCACAGCGTTCGAGACCTGT‑GCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc                                                          >  7:319914/1‑145 (MQ=21)                                                                                         ggTGCAGCACAGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                                                   <  2:231942/149‑1 (MQ=255)                                                                                              agcaCAGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATc                                              <  7:148055/149‑1 (MQ=255)                                                                                                cacaGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCg                                            >  8:203648/1‑149 (MQ=255)                                                                                                   aGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCttt                                                                          <  8:227482/116‑1 (MQ=255)                                                                                                   aGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCttt                                                                          >  7:227482/1‑116 (MQ=255)                                                                                                      tctcGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAg                                      <  6:286341/149‑1 (MQ=255)                                                                                                          gAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTgg                                  >  3:111046/1‑149 (MQ=255)                                                                                                          gAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTgg                                  >  5:65498/1‑149 (MQ=255)                                                                                                          gAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTgg                                  <  6:65498/149‑1 (MQ=255)                                                                                                          gAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTg                                   <  1:147595/148‑1 (MQ=255)                                                                                                            aCGCTGT‑GCCGTACCTGTGGGAGTGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCt                                <  1:12679/149‑1 (MQ=255)                                                                                                                tgt‑gCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCa                            >  5:183279/1‑149 (MQ=255)                                                                                                                tgt‑gCCATACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCa                            >  4:231572/1‑149 (MQ=255)                                                                                                                          ccTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTc                    <  3:168671/148‑1 (MQ=255)                                                                                                                                ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGcccc             <  1:46889/149‑1 (MQ=255)                                                                                                                                  aGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTGCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCgg           >  7:249587/1‑149 (MQ=255)                                                                                                                                   gCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGt          <  2:14210/149‑1 (MQ=255)                                                                                                                                     gggCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTGTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTg         >  6:370830/1‑148 (MQ=255)                                                                                                                                      ggCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCa       >  3:161830/1‑149 (MQ=255)                                                                                                                                           gCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCaa  >  3:119343/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                  CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGT‑GCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAA  >  NC_002947/6013745‑6014030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑