| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 91.4% | 88.6 / ‑6.6 | 35 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (11/21); minor base A (3/0); total (14/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 100.0% | 97.0 / NA | 35 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (14/21); total (14/21) | |||||||||||
CTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGC > NC_002947/1499362‑1499645 || cTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CAcg‑ccc > 1:24364/1‑148 (MQ=37) gTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCcaca < 7:323456/149‑1 (MQ=21) aTGCGGCCTGTCAGCCCCTGAACGAAGCCTTGGCACCACACCAGGACTGAGGCTGCCAAAGGGTGGGAGCGGGCGTGCCCGCTAAACTGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGa < 4:278507/148‑1 (MQ=18) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGAtt < 5:179490/149‑1 (MQ=21) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGAtt < 5:169741/149‑1 (MQ=21) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCCGGGCATGGCACCCGCGTTGCCCGTGTTAGCGGG‑CACGCCCCCGCCCACAGAcagctt > 4:145530/1‑144 (MQ=21) agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCAtt < 7:121116/148‑1 (MQ=21) cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACACGCGCCGCGGTGCATGGCACCCGCGTTGCCGGTGTTCGCCGG‑CCCGCCCGCGCCCCCACACGGATTGCCATTAATCGGGCCTATAGCTa > 3:294526/1‑149 (MQ=18) ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGAGCATGGCACCCGCTTTGCCGGTGTTAGCGGGCCCCC‑CCGCTCCCACAGACAGATTGCCAATAAGCGGGCCTAACGCTa > 6:377670/1‑148 (MQ=21) cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCggg < 7:153899/126‑1 (MQ=18) gACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttt > 5:296237/1‑149 (MQ=21) cggcggcCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCTTGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt < 4:294526/148‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt < 3:145530/149‑1 (MQ=21) cTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCTTGCCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc < 3:41082/149‑1 (MQ=21) ccAAACGGTGGGAGCAGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTc > 5:48876/1‑149 (MQ=21) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc > 5:312115/1‑149 (MQ=21) ggTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAAc < 5:377670/149‑1 (MQ=21) tGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGa < 6:67909/149‑1 (MQ=21) ggAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt < 6:296237/149‑1 (MQ=25) gAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 2:285692/149‑1 (MQ=25) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:24364/149‑1 (MQ=25) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 1:241429/149‑1 (MQ=37) gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc < 6:312115/149‑1 (MQ=37) gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc < 3:345058/149‑1 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:249691/149‑1 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGACTTTAAAGGTGTTAGCGGG‑CAAGCCCGCTCCCACAGACTGCTTGCCATTAAGCGGGCCTAACGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt > 7:242987/1‑148 (MQ=25) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 6:218831/1‑146 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 6:93077/1‑146 (MQ=37) gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc < 5:218831/149‑1 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg < 4:264438/149‑1 (MQ=37) cgcgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc > 4:35514/1‑113 (MQ=21) cgcgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc < 3:35514/113‑1 (MQ=21) gcgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGGCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCGTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg > 4:293824/1‑149 (MQ=37) gTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATc > 4:64234/1‑149 (MQ=38) gCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGCATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtct > 7:149395/1‑149 (MQ=255) gcGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgc < 7:155394/148‑1 (MQ=255) || CTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGC > NC_002947/1499362‑1499645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |