Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,499,480	2 bp→AC	intergenic (+57/‑106)	trpS → / → zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,499,480	0	C	A	100.0%	135.2 / NA	48	intergenic (+57/‑107)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/31);  total (17/31)
*	NC_002947	1,499,481	0	A	C	100.0%	139.7 / NA	48	intergenic (+58/‑106)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/31);  total (17/31)

CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATC  >  NC_002947/1499339‑1499617                                                                                                                                              ||                                                                                                                                            cGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCtt                                                                                                                                       <  2:353548/149‑1 (MQ=255) cGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCtt                                                                                                                                       >  4:119808/1‑149 (MQ=255)  gACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCttt                                                                                                                                      <  5:3444/149‑1 (MQ=255)      ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg                                                                                                                                  <  5:384685/149‑1 (MQ=255)        ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgt                                                                                                                                <  7:151947/149‑1 (MQ=255)        ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgt                                                                                                                                <  6:151312/149‑1 (MQ=255)         cGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACGACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCTGCGGTGCATGGCACCGGCTTTGCCGgtg                                                                                                                               <  4:360113/149‑1 (MQ=255)         cGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtg                                                                                                                               <  3:6033/149‑1 (MQ=255)             gTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcg                                                                                                                           >  1:249691/1‑147 (MQ=255)                cGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcg                                                                                                                         <  3:252512/148‑5 (MQ=255)                 gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTTCCGGTGttagcggg                                                                                                                       >  5:67909/1‑143 (MQ=38)                 gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg                                                                                                                        <  7:142578/148‑6 (MQ=255)                        cTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CAcg‑ccc                                                                                                              >  1:24364/1‑148 (MQ=37)                                 gTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCcaca                                                                                                      <  7:323456/149‑1 (MQ=21)                                    aTGCGGCCTGTCAGCCCCTGAACGAAGCCTTGGCACCACACCAGGACTGAGGCTGCCAAAGGGTGGGAGCGGGCGTGCCCGCTAAACTGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGa                                                                                                    <  4:278507/148‑1 (MQ=18)                                         gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGAtt                                                                                              <  5:169741/149‑1 (MQ=21)                                         gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGAtt                                                                                              <  5:179490/149‑1 (MQ=21)                                         gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCCGGGCATGGCACCCGCGTTGCCCGTGTTAGCGGG‑CACGCCCCCGCCCACAGAcagctt                                                                                              >  4:145530/1‑144 (MQ=21)                                                agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCAtt                                                                                        <  7:121116/148‑1 (MQ=21)                                                           gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg                                                                                                                        <  3:363911/106‑6 (MQ=21)                                                           gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg                                                                                                                        >  4:363911/1‑101 (MQ=21)                                                                cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACACGCGCCGCGGTGCATGGCACCCGCGTTGCCGGTGTTCGCCGG‑CCCGCCCGCGCCCCCACACGGATTGCCATTAATCGGGCCTATAGCTa                                                                       >  3:294526/1‑149 (MQ=18)                                                                 ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGAGCATGGCACCCGCTTTGCCGGTGTTAGCGGGCCCCC‑CCGCTCCCACAGACAGATTGCCAATAAGCGGGCCTAACGCTa                                                                       >  6:377670/1‑148 (MQ=21)                                                                             cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCggg                                                                                 <  7:153899/126‑1 (MQ=18)                                                                                gACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttt                                                       >  5:296237/1‑149 (MQ=21)                                                                                     cggcggcCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCTTGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt                                                  <  4:294526/148‑1 (MQ=21)                                                                                     aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt                                                  <  3:145530/149‑1 (MQ=21)                                                                                        cTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCTTGCCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc                                               <  3:41082/149‑1 (MQ=21)                                                                                           ccAAACGGTGGGAGCAGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTc                                            >  5:48876/1‑149 (MQ=21)                                                                                             aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                          >  5:312115/1‑149 (MQ=21)                                                                                                 ggTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAAc                                      <  5:377670/149‑1 (MQ=21)                                                                                                   tGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGa                                    <  6:67909/149‑1 (MQ=21)                                                                                                     ggAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt                                  <  6:296237/149‑1 (MQ=25)                                                                                                      gAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc                                 <  2:285692/149‑1 (MQ=25)                                                                                                        gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                               <  2:24364/149‑1 (MQ=25)                                                                                                          gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                             <  1:241429/149‑1 (MQ=37)                                                                                                              gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                         <  3:345058/149‑1 (MQ=37)                                                                                                              gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                         <  6:312115/149‑1 (MQ=37)                                                                                                                  ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                     <  2:249691/149‑1 (MQ=37)                                                                                                                  ccGCGAAACAGGCGCCGCGGTGCATGGCACCGACTTTAAAGGTGTTAGCGGG‑CAAGCCCGCTCCCACAGACTGCTTGCCATTAAGCGGGCCTAACGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt                      >  7:242987/1‑148 (MQ=25)                                                                                                                   cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                       >  6:218831/1‑146 (MQ=37)                                                                                                                   cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                       >  6:93077/1‑146 (MQ=37)                                                                                                                      gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc                 <  5:218831/149‑1 (MQ=37)                                                                                                                               gccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg        <  4:264438/149‑1 (MQ=37)                                                                                                                                 cgcgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                          >  4:35514/1‑113 (MQ=21)                                                                                                                                 cgcgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                          <  3:35514/113‑1 (MQ=21)                                                                                                                                  gcgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGGCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCGTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg     >  4:293824/1‑149 (MQ=37)                                                                                                                                     gTGCATGGCACCGGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATc  >  4:64234/1‑149 (MQ=38)                                                                                                                                              ||                                                                                                                                            CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGG‑CACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATC  >  NC_002947/1499339‑1499617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑