breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
read file | reads | bases | passed filters | average | longest | mapped | |
---|---|---|---|---|---|---|---|
errors | ALE-79_S79_L001_R1_001.good.fq | 389,516 | 55,819,958 | 100.0% | 143.3 bases | 149 bases | 94.1% |
errors | ALE-79_S79_L001_R2_001.good.fq | 389,516 | 55,700,798 | 100.0% | 143.0 bases | 149 bases | 94.1% |
errors | ALE-79_S79_L002_R1_001.good.fq | 378,943 | 54,202,296 | 100.0% | 143.0 bases | 149 bases | 95.6% |
errors | ALE-79_S79_L002_R2_001.good.fq | 378,943 | 54,125,348 | 100.0% | 142.8 bases | 149 bases | 94.7% |
errors | ALE-79_S79_L003_R1_001.good.fq | 397,851 | 56,899,222 | 100.0% | 143.0 bases | 149 bases | 95.5% |
errors | ALE-79_S79_L003_R2_001.good.fq | 397,851 | 56,803,028 | 100.0% | 142.8 bases | 149 bases | 95.2% |
errors | ALE-79_S79_L004_R1_001.good.fq | 364,860 | 52,166,626 | 100.0% | 143.0 bases | 149 bases | 95.3% |
errors | ALE-79_S79_L004_R2_001.good.fq | 364,860 | 52,096,932 | 100.0% | 142.8 bases | 149 bases | 94.4% |
total | 3,062,340 | 437,814,208 | 100.0% | 143.0 bases | 149 bases | 94.9% |
seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
---|---|---|---|---|---|---|---|
coverage | distribution | NC_002947 | 6,181,873 | 67.4 | 2.2 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
total | 6,181,873 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
option | limit | actual |
---|---|---|
Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 62321 |
Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 962 |
Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.048 |
reference sequence | pr(no read start) |
---|---|
NC_002947 | 0.80897 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
option | value |
---|---|
Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
option | value |
---|---|
Mode | Consensus/Mixed Base |
Ploidy | 1 (haploid) |
Consensus mutation E-value cutoff | 10 |
Consensus frequency cutoff | 0.75 |
Consensus minimum variant coverage each strand | OFF |
Consensus minimum total coverage each strand | OFF |
Consensus minimum variant coverage | OFF |
Consensus minimum total coverage | OFF |
Polymorphism E-value cutoff | 10 |
Polymorphism frequency cutoff | 0.2 |
Polymorphism minimum variant coverage each strand | OFF |
Polymorphism minimum total coverage each strand | OFF |
Polymorphism minimum variant coverage | OFF |
Polymorphism minimum total coverage | OFF |
Polymorphism bias cutoff | OFF |
Predict indel polymorphisms | YES |
Skip indel polymorphisms in homopolymers runs of | OFF |
Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
program | version |
---|---|
bowtie2 | 2.3.4.1 |
R | 3.4.4 |
step | start | end | elapsed |
---|---|---|---|
Read and reference sequence file input | 20:07:19 17 Feb 2020 | 20:08:06 17 Feb 2020 | 47 seconds |
Read alignment to reference genome | 20:08:07 17 Feb 2020 | 20:15:45 17 Feb 2020 | 7 minutes 38 seconds |
Preprocessing alignments for candidate junction identification | 20:15:45 17 Feb 2020 | 20:16:30 17 Feb 2020 | 45 seconds |
Preliminary analysis of coverage distribution | 20:16:30 17 Feb 2020 | 20:18:18 17 Feb 2020 | 1 minute 48 seconds |
Identifying junction candidates | 20:18:18 17 Feb 2020 | 20:28:43 17 Feb 2020 | 10 minutes 25 seconds |
Re-alignment to junction candidates | 20:28:43 17 Feb 2020 | 20:31:03 17 Feb 2020 | 2 minutes 20 seconds |
Resolving best read alignments | 20:31:03 17 Feb 2020 | 20:32:09 17 Feb 2020 | 1 minute 6 seconds |
Creating BAM files | 20:32:09 17 Feb 2020 | 20:33:45 17 Feb 2020 | 1 minute 36 seconds |
Tabulating error counts | 20:33:45 17 Feb 2020 | 20:34:18 17 Feb 2020 | 33 seconds |
Re-calibrating base error rates | 20:34:18 17 Feb 2020 | 20:34:20 17 Feb 2020 | 2 seconds |
Examining read alignment evidence | 20:34:20 17 Feb 2020 | 20:40:34 17 Feb 2020 | 6 minutes 14 seconds |
Polymorphism statistics | 20:40:34 17 Feb 2020 | 20:40:35 17 Feb 2020 | 1 second |
Output | 20:40:35 17 Feb 2020 | 20:41:37 17 Feb 2020 | 1 minute 2 seconds |
Total | 34 minutes 17 seconds |