| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,655,641 | Δ1 bp | 8.9% | intergenic (‑96/+56) | epd ← / ← tktA | D‑erythrose 4‑phosphate dehydrogenase/transketolase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,655,641 | 0 | T | . | 8.9% | 152.3 / 11.0 | 56 | intergenic (‑96/+56) | epd/tktA | D‑erythrose 4‑phosphate dehydrogenase/transketolase A |
| Reads supporting (aligned to +/- strand): ref base T (23/26); new base . (3/2); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01 | |||||||||||
CGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCC > NC_002947/5655497‑5655781 | cGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgt < 5:298219/149‑1 (MQ=255)cGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGCGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgt < 8:144322/149‑1 (MQ=255) cGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGa < 3:138237/148‑1 (MQ=255) cATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCggg < 6:128199/149‑1 (MQ=255) aGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGTAATTTGCGGGGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGGGGGAGCGGGCTTGCCcgcg < 8:21141/148‑1 (MQ=255) gTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCgg > 4:315385/1‑136 (MQ=37) gTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCgg < 3:315385/136‑1 (MQ=37) cAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGAGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATg > 2:300917/1‑148 (MQ=255) cTTTGTAGGGACGGGGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGGGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGTGCGGGCTTGCCCGCGAATG‑AAgg < 3:214992/149‑1 (MQ=255) tttGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGc > 3:124452/1‑149 (MQ=255) ttGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGc < 4:124452/148‑1 (MQ=255) tAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGCCAAGCCCGCTCCCACAG‑GGCTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGaca < 3:286059/149‑1 (MQ=255) ggACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAt > 7:282261/1‑136 (MQ=255) ggACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAt < 8:282261/136‑1 (MQ=255) ggCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCACCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCCACACCGCCCGCaca > 1:199074/1‑146 (MQ=255) gCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATc < 2:238016/149‑1 (MQ=255) ttCGGGGCTCACGAGACATTTCACTTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCttt < 1:41028/149‑1 (MQ=255) ttCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCtt > 5:279229/1‑148 (MQ=255) gggCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCGGTGGGAGCGGGCGTGCCCGCGAATG‑AAGGCGCCCCCGCCCGCAGCTCTTTaaa > 7:265299/1‑148 (MQ=38) ggCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAAt > 1:326517/1‑148 (MQ=255) ggCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGAGCTTTTAAt > 7:304581/1‑148 (MQ=255) gCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCa < 5:351928/149‑1 (MQ=255) cACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTc < 3:160827/149‑1 (MQ=255) gagaCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACCG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGGCCCCGCCCGCAGCTCGTTAAAtagatcg > 4:301847/1‑141 (MQ=37) gagaCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTaaa < 3:301847/140‑1 (MQ=255) aCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCATGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCca < 1:300917/148‑1 (MQ=255) aCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCcag < 6:343073/149‑1 (MQ=255) tcgtcAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑gg < 1:293880/148‑1 (MQ=255) tcAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAATCCCCCTACCACAA‑GGTTTAATTCAACCACTGTAGGAGCGGGCTTTGCCCCGAATG‑AAGGCGACACCGCCCCCCGATCTTTAAATAATTATTACACAAGCTCATA‑GGCAg > 8:306973/1‑148 (MQ=255) cTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACCCCGCCCGCAGCTCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTAcc > 5:167987/1‑147 (MQ=255) cGGAATTTGCAGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAg < 8:251770/147‑1 (MQ=255) ttGCGGTGTGGCTTTCGCGTGAAAGCCCGATCCCACAG‑GTTTTCATGAAACCTCTTTGTGAGCGGGCTTGCCCGCGAATG‑AAGGCTACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTtc < 7:306973/148‑1 (MQ=38) gCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTtctc > 4:265181/1‑148 (MQ=255) gCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCGCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATAAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGttggc > 8:18773/1‑145 (MQ=255) gtgtGGCATTCGCGGGAAAGCCCGCTCCCACAT‑GGTTTCATGCAACCTCTGTGGTAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATGAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAgg < 2:199074/149‑1 (MQ=255) tgtgGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCtt < 2:300669/98‑1 (MQ=255) tgtgGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCtt > 1:300669/1‑98 (MQ=255) gcgGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCaaa > 5:312172/1‑149 (MQ=255) gcgGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCaaa > 7:249436/1‑149 (MQ=255) gcgGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCaa > 4:232080/1‑148 (MQ=255) gggCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAAct > 8:73123/1‑149 (MQ=255) ccatgCCCCCTCCCACAG‑GGTTTATTGCACCCTCTGTGGTCGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAActct < 8:304581/145‑1 (MQ=255) cAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCtt < 2:326517/149‑1 (MQ=255) cAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCtt < 6:279229/149‑1 (MQ=255) aaGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTc > 1:150586/1‑149 (MQ=255) aaGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTc < 3:232080/149‑1 (MQ=255) cGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATGCCACACCGCAAATTCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGaa > 6:288989/1‑148 (MQ=17) cTCCCACAG‑GGTTTCATGCACCCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCTGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAAt > 8:4782/1‑149 (MQ=255) cacagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGcc < 5:136318/123‑1 (MQ=37) cacagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGcc > 6:136318/7‑129 (MQ=37) cacaG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCAAGCAGCTCATCTAGCAGTAAAGAGGATGTTATCAAGCGTGAAGACAAACTCTTCGAACaaagaa > 1:303882/1‑145 (MQ=255) acaG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCg > 3:338832/1‑148 (MQ=255) acaG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCg > 8:74202/1‑148 (MQ=255) tCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGgc < 7:254163/148‑1 (MQ=255) cATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGgcgc > 3:341706/1‑149 (MQ=255) aTGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGcc > 8:111658/1‑149 (MQ=255) | CGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAATG‑AAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTC‑GGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCC > NC_002947/5655497‑5655781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |