Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,688,515 Δ1 bp 7.0% coding (451/2061 nt) pilJ ← twitching motility protein PilJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,688,5150C. 7.0% 182.4 / 7.0 57coding (451/2061 nt)pilJtwitching motility protein PilJ
Reads supporting (aligned to +/- strand):  ref base C (25/28);  new base . (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

CCAAGGATGCGTTCGGCCAGCAACAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGTCACGCACCGCAGGTGGCGCAGGCGGCAGGCCAGTGGACTTGT  >  NC_002947/5688376‑5688655
                                                                                                                                             |                                                                                                                                              
ccAAGGATGCGTTCGGCCAGCAACAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTg                                                                                                                                      <  8:99868/149‑1 (MQ=255)
      attcGTTCGGCCAGCAACAGCTGGCGCTGGGCCACCGCAACATGAT‑‑TGGCCGGGGCACCGCTATGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCAAGGGTCGCTGCCACCTGGTGCAgag                                                                                                                                >  4:201821/4‑146 (MQ=255)
      aTGCGTTCGGCCAGCAACAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGa                                                                                                                               <  5:161414/149‑1 (MQ=255)
         cgctCGGCCAGACACAGCGGGCGCTGGGACACCTAAACCTGATCCTCAAAGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCACCAGGTGGCAGCGa                                                                                                                               <  1:308373/145‑1 (MQ=255)
         cGTTCGGCCAGCAACAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACa                                                                                                                             <  6:150572/148‑1 (MQ=255)
          gTTCGGCCAGCAACAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAg                                                                                                                            >  7:86296/1‑148 (MQ=255)
           ttCGGCCAGCAACAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCa                                                                                                                          >  2:121254/1‑149 (MQ=255)
             cGGCCAGCAACAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACg                                                                                                                        <  4:306746/149‑1 (MQ=255)
              ggCCAGCAACAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACgg                                                                                                                       >  4:175982/1‑149 (MQ=255)
                 cAGCAACAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACTGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCt                                                                                                                    <  3:201821/149‑1 (MQ=255)
                   gCAACAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGc                                                                                                                  <  4:166679/149‑1 (MQ=255)
                      aCAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCACTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCACCAGGTGGCAGCGa                                                                                                                               <  6:202622/133‑1 (MQ=255)
                      aCAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCACTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCACCAGGTGGCAGCGa                                                                                                                               >  5:202622/1‑133 (MQ=255)
                          cTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTgg                                                                                                           >  5:301224/1‑149 (MQ=255)
                              cgcTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAg                                                                                                       <  6:301224/149‑1 (MQ=255)
                                 tGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGgat                                                                                                    >  6:114375/1‑149 (MQ=255)
                                         cGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCgg                                                                                            >  4:342412/1‑149 (MQ=255)
                                              ccTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTc                                                                                       >  1:185045/1‑149 (MQ=255)
                                               cTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCt                                                                                      >  5:130798/1‑149 (MQ=255)
                                                 gAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTc                                                                                              <  3:108853/139‑1 (MQ=255)
                                                        gCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGgt                                                                                           <  2:132708/137‑1 (MQ=255)
                                                         ccGGGGCACCGCCCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAgg                                                                              >  2:17363/1‑149 (MQ=255)
                                                            gggCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGgtg                                                                                          >  3:256598/1‑134 (MQ=255)
                                                            gggCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGgtg                                                                                          <  4:256598/134‑1 (MQ=255)
                                                                  cGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCg                                                                                                               >  7:23663/1‑107 (MQ=255)
                                                                  cGCTCTGCAGAAGGATCTCGACCACTTTTTCGTATTCTACCTTCTGTTGAGGCACGGTCTCGGCGAGTGTCGCTTCCTCCTGGTGCAGCGAGAGCACTTTCTGCTCg                                                                                                               <  8:23663/107‑1 (MQ=255)
                                                                    ctctGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTc                                                                   >  1:125437/1‑149 (MQ=255)
                                                                       tGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTcgcg                                                                >  3:248465/1‑149 (MQ=255)
                                                                         cagcagGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCgcgg                                                               <  3:175982/148‑1 (MQ=255)
                                                                            cagGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTa                                                           <  1:17363/149‑1 (MQ=255)
                                                                             agGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTAc                                                          <  1:121254/149‑1 (MQ=255)
                                                                                 gctcGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGAt                                                      <  4:11815/148‑1 (MQ=255)
                                                                                     gACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGa                                                       <  4:248465/144‑1 (MQ=255)
                                                                                      aCCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCAt                                                 <  8:86296/149‑1 (MQ=255)
                                                                                       ccACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCAtc                                                <  5:114375/149‑1 (MQ=255)
                                                                                       ccACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCAtc                                                >  5:189443/1‑149 (MQ=255)
                                                                                          cTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGc                                                                                                                                 >  5:2917/1‑65 (MQ=255)
                                                                                          cTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGc                                                                                                                                 <  6:2917/65‑1 (MQ=255)
                                                                                           tttttCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGga                                                                                                     >  4:169747/1‑92 (MQ=255)
                                                                                           tttttCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGga                                                                                                     <  3:169747/92‑1 (MQ=255)
                                                                                             tttCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGt                                            <  6:130798/147‑1 (MQ=255)
                                                                                               tCGTATTCCACCTGCAGCTGCGGCAAGGTCTCGGCCAGGGTAGCTTCCACCTGGTGCAGCGACAGCACGGTCTGCTAGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTGCATAACGTCCCg                                        >  7:234346/1‑149 (MQ=255)
                                                                                                  tATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGTCACGCAc                                     >  5:132771/1‑149 (MQ=255)
                                                                                                   aTTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGTCACGCAcc                                    >  3:317240/1‑149 (MQ=255)
                                                                                                    ttCCACCTGCAGCTGCGGCACGGTCTCGGCGAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGgtgt                                                                                         >  1:30746/1‑95 (MQ=255)
                                                                                                    ttCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGgtgt                                                                                         <  2:30746/95‑1 (MQ=255)
                                                                                                    ttCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGTCACGCACCg                                   <  3:342412/149‑1 (MQ=255)
                                                                                                        aCCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGTCACGCACCGCAgg                               >  4:35031/1‑149 (MQ=255)
                                                                                                         ccTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGTCACGCACCGCAGGt                              <  3:35031/149‑1 (MQ=255)
                                                                                                            gCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTcgc                                                                 >  2:295557/1‑111 (MQ=255)
                                                                                                            gCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTcgc                                                                 <  1:295557/111‑1 (MQ=255)
                                                                                                             cAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGTCACGCACCGCAGGTgg                            <  6:189443/147‑1 (MQ=255)
                                                                                                              aGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGTCACGCACCGCAGGTGgcgc                         <  2:185045/149‑1 (MQ=255)
                                                                                                                     gCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGTCACGCACCGCAGGTGGCGCAggcggc                  >  8:239951/1‑149 (MQ=255)
                                                                                                                           tctcGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCAGGCCAGGATGATGACGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGTCACGCACCGCAGGTGGCGCAGGCGGCAGGc              >  2:82594/1‑147 (MQ=255)
                                                                                                                                   aGGGTCGGGGCCAACTGGGGCATGCTAAGCACGTGATGCTCGCTTGCCAGGTTGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTACATCTCGTCACGCACCGCAGGTGGCGCAGGCGGCAGGCCAGTGGACtt    <  8:234346/149‑1 (MQ=255)
                                                                                                                                     tgTCGCTGCACCAGGTGGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGTCACGCACCGCAGGTGGCGCAGGCGGCAGGCCAGTGGACTTGt  >  1:238380/2‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                              
CCAAGGATGCGTTCGGCCAGCAACAGCTGGCGCTGGGCCACCGCAACCTGAT‑‑TGGCCGGGGCACCGCTCTGCAGCAGGATCTCGACCACTTTTTCGTATTCCACCTGCAGCTGCGGCACGGTCTCGGCCAGGGTCGCTGCCACCTGGTGCAGCGACAGCACGGTCTGCTCGCTGGCCAGGATGATGTCGGTGTTCTTGCGCAGGTTTTCCCAGTCGCGGCGTACCGATTCCATCTCGTCACGCACCGCAGGTGGCGCAGGCGGCAGGCCAGTGGACTTGT  >  NC_002947/5688376‑5688655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: