Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,451,800	Δ1 bp	5.5%	coding (3319/3450 nt)	mfd ←	transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,451,800	0	G	.	5.5%	240.6 / 6.6	73	coding (3319/3450 nt)	mfd	transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base G (33/36);  new base . (2/2);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

CACGCATGAGTCTTCCTTAGGCAGGCTGT‑GGGGTCAGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGTCAGGCGCATCAGGTTCTTGGTCG  >  NC_002947/2451653‑2451946                                                                                                                                                     |                                                                                                                                                     cACGCATGAGTCTTCCTTAGGCAGGCTGTGGGGGTCAGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTg                                                                                                                                                      >  1:44415/1‑149 (MQ=255)   cGCATGAGTCTTCCTTAGGCAGGCTGT‑GGGGTCAGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGAt                                                                                                                                                   <  2:301927/149‑1 (MQ=255)    gCATGAGTCTTCCTTAGGCAGGCTGT‑GGGGTCAGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGATCTGGGTGGCCCGTTAGAACTTGTAACGTTTGGGCTGACCCTgatc                                                                                                                                                   >  6:222877/1‑145 (MQ=255)             ttCCTTAGGCAGGCTGT‑GGGGTCAGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAt                                                                                                                                          >  8:6851/1‑148 (MQ=255)               ccTTAGGCAGGCTGT‑GGGGTCAGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑c                                                                                                                                        <  3:234114/147‑1 (MQ=255)               ccTTAGGCAGGCTGT‑GGGGTCAGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑Ca                                                                                                                                       <  6:281828/148‑1 (MQ=255)               ccTTACGCATGCTGT‑GGGGTCAGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGTTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAt                                                                                                                                          <  6:257836/146‑1 (MQ=255)                cTTAGGCAGGCTGT‑GGGGTCAGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAgc                                                                                                                                     >  7:327399/1‑148 (MQ=255)                      caggcCGG‑GGGGTCAGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGATCTGGGGGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCT‑GATCAAGCTGATCCAGGGTCAgc                                                                                                                               <  5:222877/149‑2 (MQ=255)                         gcTGT‑GGGGTCAGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTggg                                                                                                                            <  2:19276/149‑1 (MQ=255)                                   tCAGGCGCTCGAACAGCGACTCCAGGGTATTGAAACGTTCGTCGGTGCGTTCCATCGGTACCAGGAAGCGGTAGTGGGTGGCCCCTTCGAACTTGTAAGGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGGCGACCGgt                                                                                                                   <  4:342020/149‑1 (MQ=255)                                     aGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAgg                                                                                                                                     >  4:320335/1‑129 (MQ=255)                                     aGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAgg                                                                                                                                     <  3:320335/129‑1 (MQ=255)                                       gcgcTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑Gttt                                                                                                              >  5:271785/1‑149 (MQ=255)                                        cgcTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTc                                                                                                             <  2:305079/149‑1 (MQ=255)                                         gcTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCg                                                                                                            <  6:80205/149‑1 (MQ=255)                                           tCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGt                                                                                                                               >  4:135016/1‑129 (MQ=255)                                           tCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGt                                                                                                                               <  3:135016/129‑1 (MQ=255)                                            cGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑GAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCt                                                                                                         >  2:136003/1‑149 (MQ=255)                                              aaCAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTc                                                                                                       <  5:31175/149‑1 (MQ=255)                                              aaCAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTc                                                                                                       <  7:295966/149‑1 (MQ=255)                                              aaCAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTc                                                                                                       <  3:165124/149‑1 (MQ=255)                                              aaCAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTc                                                                                                       >  1:87895/1‑149 (MQ=255)                                                      cTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTc                                                                                                 >  5:25190/1‑147 (MQ=255)                                                      cTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGa                                                                                               <  7:6851/149‑1 (MQ=255)                                                      cTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGa                                                                                               <  4:337150/149‑1 (MQ=255)                                                       tCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCT‑GATCAAGCTGATCCAGGGTCAgc                                                                                                                               <  2:92450/117‑2 (MQ=38)                                                       tCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCT‑GATCAAGCTGATCCAGGGTCAgc                                                                                                                               >  1:92450/1‑116 (MQ=38)                                                          aGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCtt                                                                                           <  2:87895/149‑1 (MQ=255)                                                            ggTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCACTTCGAACTTGTAACGTTTGGGCTGAACATGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGAACGGT‑GTTTCGGCTTCGAACTCGAGCTTGc                                                                                         >  7:158393/1‑149 (MQ=255)                                                             gTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGc                                                                                         >  4:361383/1‑148 (MQ=255)                                                             gTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGc                                                                                         >  1:88660/1‑148 (MQ=255)                                                                  gAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGt                                                                                   >  6:12159/1‑149 (MQ=255)                                                                   aaccGTCCGGCGGGGCGTTCCCTCGGACCCCGCACGCGCAACGGGGTGCCCCCTTCCAACTTCTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGGGGGGCCCCCGGG‑GTTGCGCCTTCCAACTCGACCTTGCCACCGtt                                                                                  <  2:88660/146‑1 (MQ=255)                                                                   aaaCGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGtt                                                                                  >  3:173244/1‑149 (MQ=255)                                                                     aCGTTCGTCTGGGCGCGCCATCGGTACCAGCAAGCGGAACTGGGTGGCCTCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTgg                                                                                <  8:327399/149‑1 (MQ=255)                                                                     aCGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTgg                                                                                <  4:173244/149‑1 (MQ=255)                                                                      cGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTggg                                                                               <  2:44415/149‑1 (MQ=255)                                                                          cgtcgGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCAAGCTTGCCACCGTTGGGcccc                                                                           >  6:349729/1‑149 (MQ=255)                                                                            tcgGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAgg                                                                                                                                     >  2:212611/1‑90 (MQ=255)                                                                            tcgGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAgg                                                                                                                                     <  1:212611/90‑1 (MQ=255)                                                                               gggCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATc                                                                      >  8:28127/1‑149 (MQ=255)                                                                                 gCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGa                                                                    >  3:349283/1‑149 (MQ=255)                                                                                      ccATCTGAACCAGTAAGCGGAACTGGGTGGCCCCTTCGTACTAGTAACGTTTGCGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTc                                                               <  8:158393/149‑1 (MQ=255)                                                                                           ggTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGa                                                           <  6:271785/148‑1 (MQ=255)                                                                                                cAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGcc                                                       >  8:288925/1‑147 (MQ=255)                                                                                                cAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGcc                                                       >  2:216450/1‑147 (MQ=255)                                                                                                  ggAAGCTGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGc                                                   <  5:12159/149‑1 (MQ=255)                                                                                                  ggAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGtt                                                                                  >  1:169877/1‑118 (MQ=255)                                                                                                  ggAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGtt                                                                                  <  2:169877/118‑1 (MQ=255)                                                                                                        ggAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTcc                                             >  8:121517/1‑149 (MQ=255)                                                                                                            cTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGt                                         <  7:28127/149‑1 (MQ=255)                                                                                                               ggTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCAAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGc                                       <  5:349729/148‑1 (MQ=255)                                                                                                                   gCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCtt                                  >  2:299160/1‑149 (MQ=255)                                                                                                                     cccTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCtt                                  >  4:302371/1‑147 (MQ=255)                                                                                                                     cccTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCtt                                  >  3:237996/1‑147 (MQ=255)                                                                                                                       cTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGt                              <  4:237996/149‑1 (MQ=255)                                                                                                                           gAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGAc                           <  7:288925/148‑1 (MQ=255)                                                                                                                            aaCTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGt                         >  3:171857/1‑149 (MQ=255)                                                                                                                             aCTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGt                         >  3:71334/1‑148 (MQ=255)                                                                                                                               ttGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTc                                                               <  6:103838/108‑1 (MQ=255)                                                                                                                               ttGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAg                               >  3:29728/1‑140 (MQ=255)                                                                                                                               ttGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAg                               <  4:29728/140‑1 (MQ=255)                                                                                                                               ttGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGTCAg                      >  8:104668/1‑149 (MQ=255)                                                                                                                                  tAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGTCAGgc                    <  4:171857/148‑1 (MQ=255)                                                                                                                                   aaCGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGTCAGgcg                   >  7:25906/1‑148 (MQ=255)                                                                                                                                      gTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTggg                                                                               <  3:39425/85‑1 (MQ=255)                                                                                                                                      gTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTggg                                                                               >  4:39425/1‑85 (MQ=255)                                                                                                                                            gCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGTCAGGCGCATCAGGTTc         >  3:234047/1‑149 (MQ=255)                                                                                                                                            gCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGTCAGGCGCATCAGGTTc         >  5:193626/1‑149 (MQ=255)                                                                                                                                            acTGACCCT‑GATCAAGCTGATCCAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGTCAGGCGCATCAGGTTc         >  6:54664/2‑149 (MQ=255)                                                                                                                                             cTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGTCAGGCGCATCAGGTTCt        <  7:121517/149‑1 (MQ=255)                                                                                                                                             cTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGTCAGGCGCATCAGGTTCt        <  1:136003/149‑1 (MQ=255)                                                                                                                                                aCCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGTCAGGCGCATCAGGTTCtt       <  8:25906/147‑1 (MQ=255)                                                                                                                                                    tGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGTAGTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGTCAGGCGCATCAGGTTCTTGGTCg  >  1:61019/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CACGCATGAGTCTTCCTTAGGCAGGCTGT‑GGGGTCAGGCGCTCGAACAGCGCCTCCAGGGTATTGAAACGTTCGTCGGGGCGTTCCATCGGTACCAGGAAGCGGAACTGGGTGGCCCCTTCGAACTTGTAACGTTTGGGCTGACCCTGGATCAGCTTGAT‑CAGGGTCAGTGGGTCGACCGGT‑GTTTCGGCTTCGAACTCGAGCTTGCCACCGTTGGGCCCCGCATCGACTTTCTTGATGCCGAGCTTTTCCGCGTGCAGCTTGAGTGACGTCAGGCGCATCAGGTTCTTGGTCG  >  NC_002947/2451653‑2451946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑