| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,126,645:1 | +C | 100% | intergenic (‑99/+50) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 93.0% | 136.0 / 3.2 | 43 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base C (16/24); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.85e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126505‑1126787 | gccgatgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTAACGCCAATGCGGCGGGTTATCCACCATAGCATTAGC‑GGG‑GTAA‑ccccctc > 3:38890/1‑143 (MQ=255) ttGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCg < 4:309836/149‑1 (MQ=255) gTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCGCGAATGCGGAGGTATAGCCACCATCGCCTTAGC‑GGG‑TAAACCCGCTCCGACACAGAGCCCGCCAccc > 6:347564/1‑147 (MQ=255) gcAGTGACATGTGGACCTCCTGCCAGGTGGCTGGGTGGACATTGAGCCCTGGAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCg < 8:191901/148‑1 (MQ=38) gTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCCCGAATTAGGAGGTAAATCCACCCACGCCTTAGC‑GGGTTAAA‑CCCCGCCAAAACACCCCCCGCCACCCCCACCAgggggggggg > 6:129111/1‑149 (MQ=255) aCCTCCTGCCAGGTTGTTGGGCGGCCATTGAGCCCTGTTGGCGCGGGTTTCCCCGCGAATGCGGAGGTATCGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg < 2:322778/149‑1 (MQ=38) ccAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAggg < 7:137570/134‑1 (MQ=255) cAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑TGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa < 5:152037/149‑1 (MQ=255) cAGGTTGGTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGa < 4:310387/149‑1 (MQ=255) ggTTGTTGGGGGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATa < 3:32149/149‑1 (MQ=255) gttgttGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGT‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATa < 4:38890/148‑1 (MQ=255) ggTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTAGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcga < 5:347564/149‑1 (MQ=255) aCATTCAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGcc < 3:196225/103‑1 (MQ=255) ttGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACggg < 7:200034/149‑1 (MQ=255) ttGAGCCCTGTAGGCGCGGGTTTAACCGCGAATGGGGGGGGATAGGCCCCCACGCCTTAGCGGGG‑TAAC‑CCGCGCCTACACACAGCCCGCCCCCCCAGCCAGGGGGCGGGGGGGGCGATCAGGGGGGCGGATAGCTCTCGATCGACggg > 8:190981/1‑149 (MQ=255) gAGCCCTGTAGGCGCGGGTTTTACCCCCAATTGGGAGGTTTAGGCACCATAGCATTAGC‑GGGTTAAA‑CCCCGCCGAAACAAAGCCCCCCCCCCAAGCCCGGGGGCGGGGAGGGCTATAAGGGGGGCGGATAGCTCACGATAGACcgg > 6:134990/1‑147 (MQ=255) gAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGc < 1:229383/148‑1 (MQ=255) aGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACTGGCAg < 5:129111/149‑1 (MQ=255) aGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAg < 7:90255/149‑1 (MQ=255) cccTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgc < 7:190981/149‑1 (MQ=255) gcgGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGc > 4:280855/1‑135 (MQ=255) gcgGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGc < 3:280855/135‑1 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGAc < 8:161784/121‑1 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGAc > 7:161784/1‑121 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 7:299810/1‑149 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 1:357165/1‑149 (MQ=255) gCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACg > 4:156536/1‑149 (MQ=255) ataGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATa < 5:147625/92‑1 (MQ=255) ataGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATa > 6:147625/1‑92 (MQ=255) tacgcacctTCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCTGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAc < 4:205606/144‑1 (MQ=255) taGCCACCATCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAc < 1:336467/148‑1 (MQ=255) ccaccaTCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACg > 4:77223/1‑137 (MQ=255) ccaccaTCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACg < 3:77223/137‑1 (MQ=255) ccaccaTCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 7:62671/1‑149 (MQ=255) caccaTCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGc > 5:274807/1‑149 (MQ=255) aTCGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAc < 2:357165/149‑1 (MQ=255) cGCATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACCCCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGCCcg > 7:341178/1‑149 (MQ=255) cATTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggc > 7:24270/1‑149 (MQ=255) aTTCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggcg > 8:241713/1‑149 (MQ=255) ttCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggcgg > 8:301592/1‑149 (MQ=255) tCGC‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc > 4:366666/1‑149 (MQ=255) gc‑gGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCa < 7:97478/149‑1 (MQ=255) ggg‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAg > 3:270058/1‑148 (MQ=255) gg‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTa < 7:241713/149‑1 (MQ=255) | GCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑TAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTA > NC_002947/1126505‑1126787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |