Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,392,921:1	+A	7.5%	intergenic (+91/‑95)	PP_1214 → / → ruvC	transcriptional regulator/crossover junction endodeoxyribonuclease RuvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,392,921	1	.	A	7.5%	211.8 / 11.7	53	intergenic (+91/‑95)	PP_1214/ruvC	transcriptional regulator/crossover junction endodeoxyribonuclease RuvC
Reads supporting (aligned to +/- strand):  ref base . (24/25);  new base A (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.25e-01

GAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGT  >  NC_002947/1392776‑1393068                                                                                                                                                   |                                                                                                                                                    gAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CAt                                                                                                                                                  >  1:60780/1‑149 (MQ=255) gAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CAt                                                                                                                                                  <  6:321757/149‑1 (MQ=255) gAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CAt                                                                                                                                                  >  2:190834/1‑149 (MQ=255)  aaCGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CAtt                                                                                                                                                 <  2:358531/149‑1 (MQ=255)  aaCGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CAtt                                                                                                                                                 <  4:22486/149‑1 (MQ=255)   aCGACTATGCCAATGCCCAGATAACCGACGAGATCATCGAAAACCTCGGCTAAGGTGAGTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CAttt                                                                                                                                                <  8:164199/149‑1 (MQ=39)      gcTATTCCAATGCCCAGATTTGCGACGAGATCATGGAAATCCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTACCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCaa                                                                                                                                             <  6:305190/148‑1 (MQ=255)         aTTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTACAACAGGTATTACGCC‑CATTTCAAAtg                                                                                                                                          >  7:196557/1‑149 (MQ=255)              aaTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATgtggtg                                                                                                                                     <  1:190834/149‑1 (MQ=255)                   ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATgtggtg                                                                                                                                     >  6:184837/1‑144 (MQ=255)                   ccAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATgtggtg                                                                                                                                     <  5:184837/144‑1 (MQ=255)                   ccAGATTTCCGACGAGATCATGGAAAACCTCGGCGAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTAAAGGTATTACGCC‑CATTTCAAATTTGGTGGAGt                                                                                                                                 >  1:237498/1‑148 (MQ=255)                     aGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTAcc                                                                                                                              >  3:290046/1‑149 (MQ=255)                        tttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGt                                                                                                                           <  6:111073/149‑1 (MQ=255)                        tttCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGt                                                                                                                           <  2:60780/149‑1 (MQ=255)                          tCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAg                                                                                                                         >  2:181065/1‑149 (MQ=255)                            cgacgaGAACATGGAAAACCTCTTGTAATGTGATTTGGCATGTTGTGCCAAATATGAGCGTTTGATATCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGa                                                                                                                       <  8:268483/149‑1 (MQ=9)                              acgaGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                     <  1:181065/149‑1 (MQ=255)                                 agaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCggg                                                                                                                  <  7:22202/149‑1 (MQ=255)                                 agaTCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCggg                                                                                                                  <  7:59844/149‑1 (MQ=255)                                     cATTGAAAAGCTCGGCTCAGGTGATTTGGCATTTTGTGCGAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGGC‑CATTTCCAATGTGGTGCAGTACCTGTAGGAGCGGGttt                                                                                                               <  1:226901/148‑1 (MQ=38)                                       tGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTGAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAcc                                                                                                            >  2:115681/1‑149 (MQ=255)                                        ggAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCCCTCCTACAGGGATTACGCC‑CATTTCAAATGGGGTGCAGTACCTGTAGGAGCGGGTTTAacc                                                                                                           >  6:188158/1‑149 (MQ=255)                                         gAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTAccc                                                                                                           <  4:290046/148‑1 (MQ=255)                                         gAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCcg                                                                                                          <  2:300286/149‑1 (MQ=255)                                          aaaaCCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                     >  6:140942/1‑137 (MQ=255)                                          aaaaCCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGc                                                                                                                     <  5:140942/137‑1 (MQ=255)                                              ccTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTC‑AATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAg                                                                                                    <  1:111762/149‑1 (MQ=255)                                               cTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTTAACCCGCTACTACAGGTTTTAAGCC‑CATTTAAAATGTGGGGCAGTACCTGTAGGAGCGGGGTTACCCGCGcaac                                                                                                    >  1:283912/1‑148 (MQ=255)                                                        ggTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAAACCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGgcg                                                                                            >  7:269211/1‑148 (MQ=255)                                                                   aTTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTTAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTAACGGTAGGAGCGGGTTTACCCGCGAAATGGCCGGGGCAGTTTGTtgag                                                                                >  5:311830/1‑149 (MQ=37)                                                                      tgcgcccACCTCTGCGCGTTTCACCGCGCCTGGGCCGGCCCTTGCGCGGTCAACCCCGCCCCAACCGTTTAACCCCC‑CATTTCAATTGGGGTCCCTTCCCTTTTGCCGGGGGTTAACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGAt                                                                              <  8:196557/143‑1 (MQ=255)                                                                                  tGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAAtgtg                                                                                                                                        <  4:358069/78‑1 (MQ=255)                                                                                  tGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAAtgtg                                                                                                                                        >  3:358069/1‑78 (MQ=255)                                                                                   gAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTAAGCC‑CCTTTAAAATGTGGTGGAATAACTGTAGGAGCGGGTTTAACCGCGAAAGGGCCGGCGCACTTTGTTTAAATGCATAAgtttttg                                                                 >  2:61826/1‑147 (MQ=21)                                                                                               gcgcCTGTGCCGGCCCTTTCGCGGTTAAACCCGCTCCTACCGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGGGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTAt                                                     <  5:188158/148‑1 (MQ=255)                                                                                                    tgtgCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGt                                                                                                                           <  6:176450/73‑1 (MQ=255)                                                                                                    tgtgCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGt                                                                                                                           >  5:176450/1‑73 (MQ=255)                                                                                                           gCCGTTTGGCGGGTAAACCCGCTCCTACAGTTATTACGCC‑ATTTTCAAATTTGTTGATGAACCTGTAGGTGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTa                                        <  1:61826/149‑1 (MQ=37)                                                                                                            ccgtttCGCGGGAAAACCCGCTCATACAGTTATTACGCC‑CATTCAAATTGTGGTGCAGTACCTTTCGTAGGGGTTTTACCCGCTAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAg                                       <  2:237498/146‑1 (MQ=255)                                                                                                                             ccGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcg                      >  4:317374/1‑149 (MQ=255)                                                                                                                              cGCTCCTACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTcgc                     >  3:156649/1‑149 (MQ=18)                                                                                                                                   cTACAGCTATTACGCC‑CATTTCAAATGTGCTGCAGTACCTGTAGGAGCGCGTTTACCATCGAAAGGGACGGCGCAGTTTGTAGAGATGAATCAATTTTTAGACGGGATTTATTACTCTGATTCTACATATCGCCCCCGGATCGCTCAt                 >  5:122211/1‑148 (MQ=255)                                                                                                                                    tACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCa               >  7:201251/1‑149 (MQ=255)                                                                                                                                    tACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGActct                                               <  6:266350/117‑1 (MQ=14)                                                                                                                                    tACAGGTACTGCACCACATTTGAAATG‑GGCGTAATACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGActct                                               >  5:266350/1‑117 (MQ=14)                                                                                                                                      cAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATc                                  <  5:212381/128‑1 (MQ=255)                                                                                                                                      cAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATc                                  >  6:212381/1‑128 (MQ=255)                                                                                                                                      cAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCAcc             >  8:105444/1‑149 (MQ=255)                                                                                                                                           aTTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCTACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGcaa        >  7:230206/1‑147 (MQ=255)                                                                                                                                              aCGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATgg     >  2:154062/1‑149 (MQ=255)                                                                                                                                              aCGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATg      <  8:201251/148‑1 (MQ=255)                                                                                                                                                gCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCAATGGCg   >  4:75727/1‑149 (MQ=255)                                                                                                                                                 cc‑cATTTCAAATGTGGTGCAGTACCTGTAGCAGCGGGTTTACCCGCGAAAGGGACGGCGCAGTTTGTAGCGATGCCTCATTTTTTCGACGGGCTTGATGACTCGGATTGTAGGTATCGACCCCGGCTCGCGCATCACCGTCTATGGCGt  >  2:286428/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCC‑CATTTCAAATGTGGTGCAGTACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCGCAGTTTGTAGAGATGCATCATTTTTTCGACGGGCTTTATGACTCTGATTCTAGGTATCGACCCCGGCTCGCGCATCACCGGCTATGGCGT  >  NC_002947/1392776‑1393068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑