| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,025,719:1 | +T | 11.1% | coding (719/1110 nt) | wbpZ → | glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,025,719 | 1 | . | T | 11.1% | 156.3 / 15.9 | 45 | coding (719/1110 nt) | wbpZ | glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base . (21/19); new base T (2/3); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
GCATGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTCTGCTGGAAGCGGCAATGTA > NC_002947/2025572‑2025866 | gcaTGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGgctgc‑t < 1:142332/149‑1 (MQ=255) gagcgagcGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGc > 6:58441/1‑149 (MQ=255) gcgagcgCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTC‑AGCAGCCTGCTGCTTGAGCtg < 5:18045/149‑2 (MQ=255) agcgCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAgg < 8:214246/149‑1 (MQ=255) agcgCGTTTTCCTGTTTGTACGCGCGTGGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAgg < 3:232293/149‑1 (MQ=255) gcgcTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGc > 8:36194/1‑149 (MQ=255) gcgcTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGc > 6:327049/1‑149 (MQ=255) cTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTC‑AGCAGCCTGCTGCTTGAGCtg > 6:319365/1‑134 (MQ=255) cTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTC‑AGCAGCCTGCTGCTTGAGCtg < 5:319365/135‑2 (MQ=255) gTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGtt > 6:377375/1‑149 (MQ=255) gTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGt > 7:210323/1‑148 (MQ=255) cgcgTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTgg < 8:210323/149‑1 (MQ=255) gTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCa < 5:377375/149‑1 (MQ=255) tactacAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCtt < 1:352991/120‑1 (MQ=255) tactacAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCtt > 2:352991/1‑120 (MQ=255) tacAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGc < 7:153234/146‑1 (MQ=255) tacAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGc > 8:153234/1‑146 (MQ=255) cAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGGATGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGAc > 1:295327/1‑149 (MQ=255) caGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑GGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGa < 4:3166/148‑1 (MQ=255) gggCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGa < 2:353748/149‑1 (MQ=255) gCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATg < 8:201976/146‑1 (MQ=255) gCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATg > 7:201976/1‑146 (MQ=255) tGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGACAGGAAGACGATGACa > 2:100005/1‑147 (MQ=255) cTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCgct > 3:172184/1‑149 (MQ=255) cAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTgg < 5:58441/148‑1 (MQ=255) tATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATg > 5:251836/1‑149 (MQ=255) tCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGtt > 5:189668/1‑144 (MQ=255) tCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGtt < 6:189668/144‑1 (MQ=255) tCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCa < 4:172184/149‑1 (MQ=255) aaCCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCAtt > 7:93349/1‑149 (MQ=255) aaCCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCAtt > 1:178711/1‑149 (MQ=255) aCCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTc < 8:331874/149‑1 (MQ=255) cGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCg > 5:341483/1‑148 (MQ=255) gTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTccatc > 5:89799/1‑149 (MQ=255) atcatcGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCt < 6:89799/149‑1 (MQ=255) gCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGctgctg < 2:361715/101‑1 (MQ=255) gCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGctgctg > 1:361715/1‑101 (MQ=255) cTTCCTGGAAGCGCACCTGAAGCAGCAGGGTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGGAGGCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGGTTTGTTATGCATTCGTATATCCATCCCATCTGCGTTCAGAGTCGt < 1:210257/149‑1 (MQ=255) ttCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATg < 7:169412/112‑1 (MQ=255) ttCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATg > 8:169412/1‑112 (MQ=255) cTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCgg > 1:233483/1‑149 (MQ=255) tCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCTGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCgg > 3:234044/1‑148 (MQ=255) gAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGAAAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCa < 7:36194/148‑1 (MQ=255) ctgaGCTC‑AGCAGCCTGCTGCTTGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTtct > 1:8331/4‑149 (MQ=255) cTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGGCGTTCGGCATTTCTctgct < 2:295327/149‑1 (MQ=255) ctcagcagcCTGCTGCTTGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTctgct < 2:8331/146‑1 (MQ=255) c‑tgAGCTCAGGCTTGACAATGTTCAGTTCCTGCGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATTCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTCTGCTGGAAGCGGCAATGTa < 8:93349/148‑1 (MQ=255) | GCATGAGCGAGCGCTTTTTCCTGTTTGTAGGCGCGTTGCGGTACTACAAGGGGCTGGATTACCTGCTACAGGCTGCGTCTATCAACCGTCTGCCGGTAGTGATCATCGGCGG‑TGGCTTCCTCGAAGCGCAGCTCAAGCAGCAGGCTGC‑TGAGCTCAGGCTTGACAATGTTCAGTTCCTGGGCAGCCTGGCAGACGATGACAGAGCGGCGCTGCTGGAGCTGTGTTATGCATTCGTATTTCCATCCCATCTGCGTTCCGAGTCGTTCGGCATTTCTCTGCTGGAAGCGGCAATGTA > NC_002947/2025572‑2025866 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |