| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,706,589:1 | +A | 6.9% | coding (3746/4188 nt) | PP_2369 → | leucine‑rich repeat‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,706,589 | 1 | . | A | 6.9% | 230.7 / 10.2 | 58 | coding (3746/4188 nt) | PP_2369 | leucine‑rich repeat‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (26/28); new base A (2/2); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01 | |||||||||||
CAGTGACGAGTTGCTGCTGACGCTCAGCGAGCTGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGCAGCCCAGCCATCTTTCGTACGAGAGTTTCAGTGGAGTTGGCCGTGAAGAC > NC_002947/2706443‑2706737 | cAGTGACGAGTTGCTGCTGACGCTCAGCGAGCTGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAt‑t < 4:130625/148‑1 (MQ=255) cGAGTTGCTGCTGACGCTCAGCGAGCTGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCcgcgc > 7:27327/1‑149 (MQ=255) aGTTGCTGCTGACGCTCAGCGAGCTGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCgg < 5:185273/149‑1 (MQ=255) aGTTGCTGCTGACGCTCAGCGAGCTGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCgg > 8:151937/1‑149 (MQ=255) gTTGCTGCTGACGCTCAGCGAGATGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGc < 8:194053/149‑1 (MQ=255) tgctgACGCTCAGCGAGCTGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATAtt > 8:92147/1‑149 (MQ=255) aCGCTCAGCGAGCTGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAATATGCCGCGCGGCA‑ATTGCgtt < 5:285301/149‑3 (MQ=255) aCGCTCAGCGAGCTGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGa < 7:92147/149‑1 (MQ=255) cTCAGCGAGCTGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTa < 6:170347/149‑1 (MQ=255) aGCGAGCTGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACt < 4:353529/148‑1 (MQ=255) tGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAAc > 1:10039/1‑149 (MQ=255) aGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGAt < 8:27327/149‑1 (MQ=255) ggCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCTCGGCATATTGCGGATTACTTCTCCAACGATCCCg < 8:305016/148‑1 (MQ=255) tCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTAc > 3:106803/1‑149 (MQ=255) gCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTAc < 3:192456/147‑1 (MQ=255) gCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTAc > 4:192456/1‑147 (MQ=255) cAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGtt < 8:30037/136‑1 (MQ=255) cAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGtt > 7:30037/1‑136 (MQ=255) cAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGa > 1:362481/1‑149 (MQ=255) cAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGa > 2:208659/1‑149 (MQ=255) ccGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTAt > 3:314080/1‑149 (MQ=255) cGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGATGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCCGATTACTTCTCGAACGATACCGTTGAGGTTTAACTGACATATc > 5:157666/1‑149 (MQ=255) gTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATcgc < 6:324651/149‑1 (MQ=255) gTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATcgc > 7:180466/1‑149 (MQ=255) gCCTGTACCGGGTTACAGAAGGAGCGGGCGCTGGGCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGTTTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAg < 6:157666/149‑1 (MQ=255) ccGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCTGTTGAGGTTTACCTGACCTATCGCGTCAgg > 1:297747/1‑149 (MQ=255) gTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATcgcg < 8:260414/141‑1 (MQ=255) gTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATcgcg > 7:260414/1‑141 (MQ=255) ccGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGc < 4:106803/148‑1 (MQ=255) gTTACAGAAGGAGCGGGCGCTGGTCAGGCGGGGGAGTTCGTTCTTCCGCCTGGACAAAGTCAACGGAAT‑TGCCTCGCGGCATATTGCGGATTAGTTCTCGAACTATCCCGGTGTGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATa < 2:181948/149‑1 (MQ=255) aGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAAc > 5:201655/1‑98 (MQ=255) aGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAAc < 6:201655/98‑1 (MQ=255) aGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGt < 7:110465/130‑1 (MQ=255) aGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGt > 8:110465/1‑130 (MQ=255) gAAGGAGCGGGCGCTGGTCAGGCTGGGGCTTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGAGTACTTCTAGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGAGAGtctc > 8:188840/1‑148 (MQ=255) aaGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAATGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGa > 6:272586/1‑149 (MQ=255) aaGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGa > 1:13708/1‑149 (MQ=255) ggAGCGGGCGCTGGTCAGGCTGTGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGAcc > 7:233855/1‑149 (MQ=255) gcgcTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGt < 4:155105/149‑1 (MQ=255) gcTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCTGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGc < 2:297747/149‑1 (MQ=255) gcTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTg > 7:177171/1‑148 (MQ=255) gTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGcagcc < 4:314080/149‑1 (MQ=255) aGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGcagccca < 2:13708/148‑1 (MQ=255) aGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGcagccca < 8:177171/148‑1 (MQ=255) gggCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGCAGCCCAGCCAt < 4:205632/147‑1 (MQ=255) gggCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGCAGCCCAGCCATCt < 8:180466/149‑1 (MQ=255) gCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATc > 7:281199/1‑74 (MQ=255) gCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATc < 8:281199/74‑1 (MQ=255) ttCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGCAGCCCAGCCATCTTTCGTACGagag > 3:27589/1‑149 (MQ=255) gCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGcagccca > 5:313556/1‑126 (MQ=255) gCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGcagccca < 6:313556/126‑1 (MQ=255) gACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGCAGCCCAGCCATCTTTCGTACGAGAGTTTCAGt < 7:151937/147‑1 (MQ=255) aaaGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGc < 4:332698/89‑1 (MQ=255) aaaGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGc > 3:332698/1‑89 (MQ=255) gTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGCAGCCCAGCCATCTTTCGTACGAGAGTTTCAg > 1:238419/1‑140 (MQ=255) gTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGCAGCCCAGCCATCTTTCGTACGAGAGTTTCAg < 2:238419/140‑1 (MQ=255) atccGCAATATGCCGCGCGGCA‑ATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGcagcc > 5:109520/4‑111 (MQ=39) atccGCAATATGCCGCGCGGCA‑ATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGcagcc < 6:109520/108‑1 (MQ=39) tccGCAATATGCCGCGCGGCA‑ATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGCAGCCCAGCCATCTTTCGTACGAGAGTTTCAGTGGAGTTGGCCg > 1:264909/3‑149 (MQ=255) t‑tGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGCAGCCCAGCCATCATTCGTACGAGAGTTTCAGAGGAGTTGGACGTGAAGAc > 8:149868/1‑149 (MQ=255) | CAGTGACGAGTTGCTGCTGACGCTCAGCGAGCTGGAGGTTGGCGCGCTGGTCGCCAAGGCCGGTACAGCCGGTACCGGGTTACAGAAGGAGCGGGCGCTGGTCAGGCTGGGGCGTTCGTTGTTCCGCCTGGACAAAGTCAACGCAAT‑TGCCGCGCGGCATATTGCGGATTACTTCTCGAACGATCCCGTTGAGGTTTACCTGACCTATCGCGTCAGGCTGGCAGATAGTCTCGACCTTCCCGTGCAGCCCAGCCATCTTTCGTACGAGAGTTTCAGTGGAGTTGGCCGTGAAGAC > NC_002947/2706443‑2706737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |