Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,845,703:1 +G 100% intergenic (‑70/+28) PP_3394 ← / ← PP_3395 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,845,7031.G97.5% 131.0 / ‑3.2 40intergenic (‑70/+28)PP_3394/PP_33953‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (20/19);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑C‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTC  >  NC_002947/3845557‑3845835
                                                                                                                                                    |                                                                                                                                    
gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑C‑gg                                                                                                                                    <  5:17662/149‑1 (MQ=255)
 ctgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑C‑ggg                                                                                                                                   <  7:60101/149‑2 (MQ=255)
       tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATc                                                                                                                              <  5:76012/149‑1 (MQ=255)
         ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATcc                                                                                                                             <  7:241981/148‑1 (MQ=255)
         ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCa                                                                                                                            <  7:163947/149‑1 (MQ=255)
         ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCa                                                                                                                            >  7:177229/1‑149 (MQ=255)
          gCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCAt                                                                                                                           >  3:120197/1‑149 (MQ=255)
           cATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCTGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATc                                                                                                                          <  1:320487/149‑1 (MQ=255)
             tGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGt                                                                                                                        >  8:145375/1‑149 (MQ=255)
                tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAg                                                                                                                     >  3:252425/1‑149 (MQ=255)
                  aCTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGc                                                                                                                    >  5:167194/1‑148 (MQ=255)
                    ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCAc                                                                                                                 >  3:147922/1‑149 (MQ=255)
                       cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGa                                                                                                               <  3:254396/148‑1 (MQ=255)
                       cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGa                                                                                                               <  8:177229/148‑1 (MQ=255)
                       cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGa                                                                                                               <  5:354092/148‑1 (MQ=255)
                       cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGAt                                                                                                              <  3:199133/149‑1 (MQ=255)
                       cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGAt                                                                                                              <  7:145375/149‑1 (MQ=255)
                       cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGAt                                                                                                              <  6:47195/149‑1 (MQ=255)
                        aGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATg                                                                                                             >  2:218277/1‑149 (MQ=255)
                          gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATgg                                                                                                            <  8:69520/148‑1 (MQ=255)
                          gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATgg                                                                                                            >  7:69520/1‑148 (MQ=255)
                          gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGa                                                                                                           <  5:213135/149‑1 (MQ=255)
                            tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGAt                                                                                                          >  6:201513/1‑148 (MQ=255)
                                cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCCTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCa                                                                                                        <  5:72332/146‑1 (MQ=255)
                                 aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCAACGTCAGCCACGATGGATCAGccc                                                                                                    >  7:204821/1‑149 (MQ=255)
                                   gCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTTTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATACATCGTCAGCCACGAAGGATCAGccccc                                                                                                  >  3:67472/1‑147 (MQ=255)
                                                 cGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCg                                                                                    >  3:129572/1‑149 (MQ=255)
                                                    cTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTg                                                                                 <  5:201513/149‑1 (MQ=255)
                                                     ttCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGt                                                                                  >  4:231874/1‑147 (MQ=255)
                                                       cATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGt                                                                                  <  3:231874/145‑1 (MQ=255)
                                                            attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCa                                                                                                        <  6:136650/118‑1 (MQ=255)
                                                            attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCa                                                                                                        >  5:136650/1‑118 (MQ=255)
                                                                ttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCCCGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCa                                                                               >  7:144144/1‑139 (MQ=255)
                                                                           aTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcagc                                                                         >  5:162004/1‑134 (MQ=255)
                                                                                      tctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg                                                                   <  8:105805/129‑1 (MQ=255)
                                                                                      tctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg                                                                   >  7:105805/1‑129 (MQ=255)
                                                                                                                      ggATGACGGGCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg                >  4:143224/1‑148 (MQ=255)
                                                                                                                             gggCAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc        >  5:228544/1‑149 (MQ=255)
                                                                                                                             gggCAGAAAAGACGGGTTTCG‑CGGGCTGATCCATCGGCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc        <  5:112878/149‑1 (MQ=255)
                                                                                                                               gCAGAAAAGACGTCTTTCGCC‑GGCTGATCCAACGTCACCCACGATTGATCAGACCCCCCCCTGACAGGCCCGTGCACGCATCAAAAGGTGGTCAAGCAGCCGCCGAGCAGACAGCGAGAGCGTGTGCCGGTCCCCCATGCAGATCACg       >  2:51949/1‑148 (MQ=255)
                                                                                                                                cAGAAAAGACGTGTTTCG‑CGGGCTGATCCATCGGCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGaa     <  1:218277/149‑1 (MQ=255)
                                                                                                                                   aaaaGACGTGTTTCG‑CGGGCTGATCCATCGTCCGCCACGATGGATCAGCCCGCGCCCTGCCCGGCCGGGGCCCGCAGCAACAGGTGGTCAAGCAGGCGCCGCGCCGCCAGCGAGAGCGTGTGCCGGTCGCGCAGGCAGAGGACgcaatc  >  2:240208/1‑144 (MQ=255)
                                                                                                                                                    |                                                                                                                                    
GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCG‑C‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTC  >  NC_002947/3845557‑3845835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑