New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	5585676 =	49 (0.830)	5 (0.090)	3/250	NT	9.5%	coding (56/2259 nt)	parC	DNA topoisomerase IV subunit A
	?	NC_002947	5585713 =	48 (0.850)					coding (19/2259 nt)	parC	DNA topoisomerase IV subunit A

GTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTCAAGTTGTTGCAGGGCACTCATG  >  NC_002947/5585531‑5585817                                                                                                                                                    |                                                                                                                                               gtgCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTca                                                                                                                                              <  1:176884/148‑1 (MQ=255) catcgtggtgccgggcctggatgggtccaccgtactgctgggcatgagtgccctgcaacaacttgaatttacccagcgcggtggcaccatgctgctgcgccagaacctgaaataatgacgaggcccgcatgagcgactcactggaact                                                                                                                                                <  8:95355‑M1/1‑1 (MQ=255)  tgCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTcag                                                                                                                                             <  6:246368/148‑1 (MQ=255)     ttGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTcagccag                                                                                                                                         >  1:23068/1‑149 (MQ=255)         catcggcatcgAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCagcgag                                                                                                                                     >  6:155513/1‑149 (MQ=255)                atcgAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGt                                                                                                                               >  4:331199/1‑148 (MQ=255)                       cccAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGcc                                                                                                                       >  4:127989/1‑149 (MQ=255)                             tCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAg                                                                                                                 >  7:251577/1‑149 (MQ=255)                                 tCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCt                                                                                                              >  1:231822/1‑148 (MQ=255)                                 tCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCt                                                                                                              >  1:330566/1‑148 (MQ=255)                                   aTGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAg                                                                                                           >  2:88742/1‑149 (MQ=255)                                   aTGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGACAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAg                                                                                                           >  4:133938/1‑149 (MQ=255)                                   aTGGCGTAGACGATGCGTCGCTACACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAg                                                                                                           >  8:250352/1‑149 (MQ=255)                                         tAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAg                                                                                                     <  2:231822/149‑1 (MQ=255)                                         ggcatgagtgccctgcaacaacttgaatttacccagcgcggtggcaccatgctgctgcgccagaacctgaaataatgacgaggcccgcatgagcgactcactggaacTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTc                                                                                                        >  7:340683‑M1/108‑148 (MQ=255)                                             cGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATTAACACGTAAATGGTTTACACATGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGt                                                                                                    <  1:299680/148‑1 (MQ=255)                                              gATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGt                                                                                                >  4:241424/1‑149 (MQ=255)                                              gATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGt                                                                                                >  5:122469/1‑149 (MQ=255)                                                  cGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGc                                                                                             <  5:375475/148‑1 (MQ=255)                                                        ttcACCGGCTTCAGGCCGTCGGCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCg                                                                                      <  5:65222/147‑1 (MQ=255)                                                         gCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCgg                                                                                     >  4:189920/1‑149 (MQ=255)                                                          cACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCgg                                                                                     <  5:275256/148‑1 (MQ=255)                                                          cACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCgg                                                                                     <  8:130728/148‑1 (MQ=255)                                                           aCCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGc                                                                                   <  2:367593/149‑1 (MQ=255)                                                           aCCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATCGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGc                                                                                   <  2:23068/149‑1 (MQ=255)                                                            ccGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGcc                                                                                  >  2:51675/1‑149 (MQ=255)                                                                cTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGt                                                                              <  3:331199/149‑1 (MQ=255)                                                                      gCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCattat                                                                        >  6:280114/1‑149 (MQ=255)                                                                          tCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCa                                                                    <  1:88742/149‑1 (MQ=255)                                                                            gCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCgg                                                                                     <  2:144442/130‑1 (MQ=255)                                                                            gCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCgg                                                                                     >  1:144442/1‑130 (MQ=255)                                                                            gCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAgg                                                                  <  1:183610/149‑1 (MQ=255)                                                                            gCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAg                                                                   <  5:155513/148‑1 (MQ=255)                                                                            gCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAg                                                                   <  8:251577/148‑1 (MQ=255)                                                                               gATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTc                                                               >  4:348577/1‑149 (MQ=255)                                                                                        cAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCgcagc                                                      <  3:127989/149‑1 (MQ=255)                                                                                         aaGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTTTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTTGCgcagca                                                     <  3:133938/149‑1 (MQ=255)                                                                                              aCGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATg                                               >  4:110070/1‑149 (MQ=255)                                                                                               cGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATgg                                              <  3:189920/149‑1 (MQ=255)                                                                                                           aCGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCt                                  <  4:208208/149‑1 (MQ=255)                                                                                                                 aTGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTa                             >  2:22537/1‑148 (MQ=255)                                                                                                                     agt‑‑agtTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTc                        <  7:250352/149‑1 (MQ=255)                                                                                                                      gt‑‑agtTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCACGCATGGTGCCACCGCGCTGGGTAAATTc                        <  1:22537/149‑1 (MQ=255)                                                                                                                       t‑‑agtTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTCaa                      <  5:280114/149‑1 (MQ=255)                                                                                                                           gtTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCCGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTCAAgt                    >  4:127754/1‑149 (MQ=255)                                                                                                                           gtTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTCAAgt                    >  3:204761/1‑149 (MQ=255)                                                                                                                             ccgcatgagcgactcactggaacTCAGTCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGa                                                                                                            >  1:44078‑M1/24‑60 (MQ=255)                                                                                                                             ccgcatgagcgactcactggaacTCAGTCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGa                                                                                                            >  1:38417‑M1/24‑60 (MQ=255)                                                                                                                             ccgcatgagcgactcactggaacTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGa                                                                                                            <  2:44078‑M1/37‑1 (MQ=255)                                                                                                                             ccgcatgagcgactcactggaacTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGa                                                                                                            <  2:38417‑M1/37‑1 (MQ=255)                                                                                                                                 gTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTCAAGttgttg               >  7:165278/1‑148 (MQ=255)                                                                                                                                   aGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTCAAGTTGTTGCAg            >  7:95355/1‑149 (MQ=255)                                                                                                                                    ggCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTCAAGTTGTTGCAg            <  3:348577/148‑1 (MQ=255)                                                                                                                                       cTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTCAAGTTGTTGCAGGGCa        <  3:110070/149‑1 (MQ=255)                                                                                                                                        tGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTCAAGTTGTTGCAGGGCAc       >  8:6660/1‑149 (MQ=255)                                                                                                                                           tCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTCAAGTTGTTGCAGGGCACTc     <  3:241424/148‑1 (MQ=255)                                                                                                                                             ggTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTCAAGTTGTTGCAGGGCACTCATg  >  6:20035/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                               GTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGT‑‑AGTTGAGGTAGGCCTGTTCGGTGAAGTCAGCCAGCGAGCGGCGTTCGACGCCGTCCAGGCTGAGTTCCAGTGAGTCGCTCATGCGGGCCTCGTCATTATTTCAGGTTCTGGCGCAGCA‑GCATGGTGCCACCGCGCTGGGTAAATTCAAGTTGTTGCAGGGCACTCATG  >  NC_002947/5585531‑5585817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.